CAJ | 학술논문

NanoString数字化基因分析系统是最新的多重基因定量检测技术.此技术可直接检测条形码探针标记的单个mRNA转录子,并通过数字计数进行定量;它仅需100 ng的RNA即可对逾八百个特异的mRNA转录子进行准确的定量;它无需酶促和扩增过程,其检测的敏感性和准确性与实时荧光定量PCR(RQ-PCR)技术相当.近年来,NanoString技术的特点及优势使其被越来越广泛地应用到生物医学前沿领域,包括高通量基因表达结果验证、基因表达谱研究、基因调控网络研究、临床疾病分子分型及诊断预后等领域.
NanoString수자화기인분석계통시최신적다중기인정량검측기술.차기술가직접검측조형마탐침표기적단개mRNA전록자,병통과수자계수진행정량;타부수100 ng적RNA즉가대유팔백개특이적mRNA전록자진행준학적정량;타무수매촉화확증과정,기검측적민감성화준학성여실시형광정량PCR(RQ-PCR)기술상당.근년래,NanoString기술적특점급우세사기피월래월엄범지응용도생물의학전연영역,포괄고통량기인표체결과험증、기인표체보연구、기인조공망락연구、림상질병분자분형급진단예후등영역.
NanoString nCounter Analysis System is a newly developed gene expression detection platform that directly measures multiplexed mRNA levels through digital counting of individual mRNA transcripts.This technology uses as little as 100 ng of RNA and can obtain accurate gene quantitative data from up to 800 genes in one reaction.It requires no reverse transcription,enzymes and amplifications,and its sensitivity and accuracy are comparable to real time quantitative PCR.NanoString technology has been more and more extensively used in frontiers of biomedical research and clinics such as in validation of data from high-throughput platforms,gene expression profiling,gene regulatory network,molecular subtyping,diagnosis and prognosis of diseases.