CAJ | 학술논문

目的研究脉冲电磁场(PEMF)对小鼠成骨细胞上与造血干细胞(HSC)归巢、增殖有关基因的作用.方法 9周龄C57BL/6J小鼠,采用频率70 Hz、电磁场强度2 mT的PEMF作用4～5周,每周作用6d,对照组小鼠不接受PEMF作用.作用结束后,取双侧股骨和胫骨,胶原酶消化、分离细胞,再经磁珠和流式分选、收集ALCAM+Sca-1-成骨细胞,最后通过表达谱芯片和定量PCR检测ALCAM+Sca-1-成骨细胞上与HSC归巢、增殖有关的基因.结果 PEMF作用下,成骨细胞上Jagl(其对应HSC上的受体为Notch)和Ang-1(其对应HSC上的受体为Tie-2)的表达增强.结论 PEMF可能通过对ALCAM+Sca-1-成骨细胞上与HSC归巢和自我更新有关基因的作用,调控HSC.
목적 연구맥충전자장(PEMF)대소서성골세포상여조혈간세포(HSC)귀소、증식유관기인적작용.방법 9주령C57BL/6J소서,채용빈솔70 Hz、전자장강도2 mT적PEMF작용4～5주,매주작용6d,대조조소서불접수PEMF작용.작용결속후,취쌍측고골화경골,효원매소화、분리세포,재경자주화류식분선、수집ALCAM+Sca-1-성골세포,최후통과표체보심편화정량PCR검측ALCAM+Sca-1-성골세포상여HSC귀소、증식유관적기인.결과 PEMF작용하,성골세포상Jagl(기대응HSC상적수체위Notch)화Ang-1(기대응HSC상적수체위Tie-2)적표체증강.결론 PEMF가능통과대ALCAM+Sca-1-성골세포상여HSC귀소화자아경신유관기인적작용,조공HSC.
Objective To investigate the effects of pulsed electromagnetic fields (PEMF) on homing and proliferation-related genes of mouse osteoblasts.Methods 9 week-old C57BL/6 mice were treated with PEMF (70 Hz,1 mT) for 4～5 weeks,while mice in control group didn't not receive PEMF.Bone marrow cells of femurs and tibias were flushed out,and the bones were minced and incubated at 37 ℃ with a type Ⅰ collagenase.Bone associated mononuclear cells (MNCs) were isolated via density centrifugation with Lymphoprep.Magnetic cell sorting was used before flow-cytometric sorting,and the ALCAM+Sca-1-cells were collected.The homing and proliferation-related genes expressed in ALCAM+Sca-1-cells were detected with high throughput microarray and RT-PCR.Results The expression of Jag1 and Ang-1 in mouse osteoblasts increased under the effects of PEMF.Conclusions PEMF may have regulation effects on HSC (hematopoietic stem cell) survival through modulating the homing and proliferationrelated genes in ALCAM+Sca-1-osteoblasts.