CAJ | 학술논문

目的探讨光动力疗法(PDT)对牙骨质片及种植体表面牙龈卟啉单胞菌(Pg)菌斑生物膜的杀灭效果,获得有效杀灭Pg的光敏剂和激光照射的合理剂量.方法选用Pg标准菌株分别联合经全唾液处理后的无菌牙骨质片和无菌种植体共同培养,建立简易的体外人工牙骨质片和种植体表面细菌附着模型.以血卟啉单甲醚(HMME)为光敏剂,波长630 nm半导体激光为光源,照射牙骨质片表面细菌附着模型60 s后,计算菌落形成单位(CFU),筛选出最佳激光能量密度(EDL)和HMME剂量,以筛选的合理剂量照射种植体表面细菌附着模型,并用扫描电镜观察PDT对种植体表面菌斑生物膜的影响.结果当EDL为12J/cm2、HMME质量浓度为25 μg/ml时,PDT可有效杀灭Pg菌斑生物膜((13.00±5.00) CFU),且扫描电镜观察到种植体表面无损伤.结论 630nm半导体激光联合HMME介导的光动力疗法(HMME-PDT)对Pg菌斑生物膜有良好的杀灭效果,本实验使用的EDL与HMME剂量较小,有望作为临床治疗剂量.
목적 탐토광동력요법(PDT)대아골질편급충식체표면아간계람단포균(Pg)균반생물막적살멸효과,획득유효살멸Pg적광민제화격광조사적합리제량.방법 선용Pg표준균주분별연합경전타액처리후적무균아골질편화무균충식체공동배양,건립간역적체외인공아골질편화충식체표면세균부착모형.이혈계람단갑미(HMME)위광민제,파장630 nm반도체격광위광원,조사아골질편표면세균부착모형60 s후,계산균락형성단위(CFU),사선출최가격광능량밀도(EDL)화HMME제량,이사선적합리제량조사충식체표면세균부착모형,병용소묘전경관찰PDT대충식체표면균반생물막적영향.결과 당EDL위12J/cm2、HMME질량농도위25 μg/ml시,PDT가유효살멸Pg균반생물막((13.00±5.00) CFU),차소묘전경관찰도충식체표면무손상.결론 630nm반도체격광연합HMME개도적광동력요법(HMME-PDT)대Pg균반생물막유량호적살멸효과,본실험사용적EDL여HMME제량교소,유망작위림상치료제량.
Objective To evaluate the bactericidal effect of diode laser on Porphyromonas gingivalis (Pg),and to explore an optimized protocol for a safe dose of photodynamic therapy (PDT) to eliminate periodontal pathogens as well as the impact on the implant surfaces,so as to provide theoretical and experimental basis for PDT in periimplantitis therapy.Methods Artificial in vitro models were formatted by culturing Pg standard strain and ITI (International Team for Implantology) implants together in CDC broth.Then artificial in vitro models were treated by different doses of hematoporphyrin monomethyl ether (HMME) and different energy density of laser (EDL) for 60 s.The cultures were counted by colony form unit (CFU),and SPSS 17.0 statistical software was used for data statistical analysis to select the best EDL and HMME dose.Finally,ITI implants were observed by scanning electron microscope (SEM) to evaluate the impact of HMME-PDT on Pg of implant surfaces.Results When EDL was 12 J/cm2 and mass concentration of HMME was 25 μg/ml,SEM observations showed that PDT could effectively kill Pg ((13.00±5.00) CFU)without damaging the implant surfaces.Conclusions PDT therapy combining 630 nm diode laser with photosensitizer HMME have good bactericidal effect on Pg,and the EDL and HMME dose is as small as the clinical applicable safe dose.