CAJ | 학술논문

目的激光照射剂量和光敏剂的种类及浓度可明显影响光动力疗法(PDT)的治疗效果.本研究以BALB/c荷瘤小鼠为对象,探讨不同激光照射剂量对PDT治疗喉癌效果的影响.方法取48只皮下接种人喉癌上皮细胞Hep-2的BALB/c荷瘤鼠,分成6组,其中1组为对照,其余5组尾静脉注射血卟啉单甲醚(HMME)后3h分别以30、60、120、240、480 J/cm2能量的波长630 nm激光照射肿瘤组织.隔日观察肿瘤大小、体质量,并在PDT后30 d取肿瘤组织做病理切片.结果与对照组相比,所有剂量组PDT都使肿瘤消除或部分消除,疗效与剂量呈正相关,120和480 J/cm2组小鼠肿瘤完全治愈.瘤损部位在PDT作用后1d出现结痂,5d结痂变硬变黑,同时肿瘤开始消退,30 d左右肿瘤完全消失.结论 PDT是治疗小鼠喉癌非常有前景的方法,其最佳照射剂量是120 J/cm2.
목적 격광조사제량화광민제적충류급농도가명현영향광동력요법(PDT)적치료효과.본연구이BALB/c하류소서위대상,탐토불동격광조사제량대PDT치료후암효과적영향.방법 취48지피하접충인후암상피세포Hep-2적BALB/c하류서,분성6조,기중1조위대조,기여5조미정맥주사혈계람단갑미(HMME)후3h분별이30、60、120、240、480 J/cm2능량적파장630 nm격광조사종류조직.격일관찰종류대소、체질량,병재PDT후30 d취종류조직주병리절편.결과 여대조조상비,소유제량조PDT도사종류소제혹부분소제,료효여제량정정상관,120화480 J/cm2조소서종류완전치유.류손부위재PDT작용후1d출현결가,5d결가변경변흑,동시종류개시소퇴,30 d좌우종류완전소실.결론 PDT시치료소서후암비상유전경적방법,기최가조사제량시120 J/cm2.
Objective The dose and type of light and photosensitizer could seriously affect the curative effect of photodynamic therapy (PDT).The purpose of this study was to observe whether or not PDT with hematoporphyrin monomethyl ether (HMME) can cure laryngocarcinoma in the solid tumor model,and to define the proper laser amount for killing the cancer cells.Methods Forty eight BALB/c mouse models with subcutaneous Hep-2 laryngeal carcinomas were prepared.Mice were divided into six groups depending on the amount of laser received from 30 J/cm2 to 480 J/cm2 including a control group,tumor size in each group was between 8 mm and 10 mm.Tail vein injection were given with HMME prior to applying the laser light,and then illumination was carried out on the tumor at 3 h after HMME administration.Tumor volume,animal weight and histopathologic changes were observed after PDT.Results All mice apparently showed positive results via PDT,and the cancer had been cured in 120 J/cm2 and 480 J/cm2 groups.The laryngeal cancer lesions began to form scab 1 d after PDT and the scab became hard and black after 5 d.The tumor regression began simultaneously and completed around 30 d after PDT.Conclusions PDT may treat laryngeal cancers which sized less than 10 mm in mouse models.The optimum energy to destruct the laryngeal cancer cells may be 120 J/cm2.