国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2014年
3期
151-155,封3
,共6页
生长因子%壳聚糖纳米粒子%壳聚糖膜%单向缓释
生長因子%殼聚糖納米粒子%殼聚糖膜%單嚮緩釋
생장인자%각취당납미입자%각취당막%단향완석
Growth factor%Chitosan nanoparticles%Chitosan membrane%One-way release-controlled
目的 将壳聚糖纳米粒子与壳聚糖单向缓释膜结合,制备得到负载纳米粒子的壳聚糖单向缓释膜,并对其理化性质及体外释放性能进行研究.方法 采用反相乳化法制备壳聚糖纳米粒子,采用流延法制备壳聚糖单向缓释膜,通过透射电镜、扫描电镜和粒度分析仪检测纳米粒子的形态、大小及壳聚糖单向缓释膜表面形貌,MTT法检测其生物相容性,荧光显微镜观察纳米粒子在壳聚糖单向缓释膜中的分布,体外释放实验检测单向缓释膜的缓释性能.结果 4种水溶性壳聚糖纳米粒子呈球状且粒径均一,其中类透明质酸壳聚糖纳米粒子平均粒径为(255.40±39.10) nm,具有最好的生物相容性及缓释性能.壳聚糖单向缓释膜外层膜致密,内层膜疏松,纳米粒子在内层膜中均匀分布,体外释放实验证明,壳聚糖单向缓释膜为单向释放,且具有较好的缓释性能.结论 包载纳米粒子的壳聚糖单向缓释膜具有较好的缓释和单向控释性能,在生长因子类药物载体方面有一定应用前景.
目的 將殼聚糖納米粒子與殼聚糖單嚮緩釋膜結閤,製備得到負載納米粒子的殼聚糖單嚮緩釋膜,併對其理化性質及體外釋放性能進行研究.方法 採用反相乳化法製備殼聚糖納米粒子,採用流延法製備殼聚糖單嚮緩釋膜,通過透射電鏡、掃描電鏡和粒度分析儀檢測納米粒子的形態、大小及殼聚糖單嚮緩釋膜錶麵形貌,MTT法檢測其生物相容性,熒光顯微鏡觀察納米粒子在殼聚糖單嚮緩釋膜中的分佈,體外釋放實驗檢測單嚮緩釋膜的緩釋性能.結果 4種水溶性殼聚糖納米粒子呈毬狀且粒徑均一,其中類透明質痠殼聚糖納米粒子平均粒徑為(255.40±39.10) nm,具有最好的生物相容性及緩釋性能.殼聚糖單嚮緩釋膜外層膜緻密,內層膜疏鬆,納米粒子在內層膜中均勻分佈,體外釋放實驗證明,殼聚糖單嚮緩釋膜為單嚮釋放,且具有較好的緩釋性能.結論 包載納米粒子的殼聚糖單嚮緩釋膜具有較好的緩釋和單嚮控釋性能,在生長因子類藥物載體方麵有一定應用前景.
목적 장각취당납미입자여각취당단향완석막결합,제비득도부재납미입자적각취당단향완석막,병대기이화성질급체외석방성능진행연구.방법 채용반상유화법제비각취당납미입자,채용류연법제비각취당단향완석막,통과투사전경、소묘전경화립도분석의검측납미입자적형태、대소급각취당단향완석막표면형모,MTT법검측기생물상용성,형광현미경관찰납미입자재각취당단향완석막중적분포,체외석방실험검측단향완석막적완석성능.결과 4충수용성각취당납미입자정구상차립경균일,기중류투명질산각취당납미입자평균립경위(255.40±39.10) nm,구유최호적생물상용성급완석성능.각취당단향완석막외층막치밀,내층막소송,납미입자재내층막중균균분포,체외석방실험증명,각취당단향완석막위단향석방,차구유교호적완석성능.결론 포재납미입자적각취당단향완석막구유교호적완석화단향공석성능,재생장인자류약물재체방면유일정응용전경.
Objective To prepare and characterize nanoparticles in-loaded one-way release-controlled chitosan membrane,and to explore the release-controlled rule of the film in vitro.Methods The chitosan nanoparticles were prepared by inverse crosslinking-emulsion method.The one-way release-controlled membrane was prepared by a casting method.Transmission electron microscope (TEM) was used to evaluate the morphological properties and particle size analyzer was used to analyze particle size distribution.The morphology of the membrane was inspected through scanning electron microscope (SEM).MTT assay was applied to determine the biological safety of chitosan nanoparticles.The distribution of the nanoparticles was observed by fluorescence microscope.The in vitro release studies were adopted to evaluate the release-controlled rule.Results The four kinds of nanoparticles had spherical shapes and uniform particle size.The size of the hyaluronic acid-coated chitosan nanoparticle was (255.40±39.10) nm.Hyaluronic acid-coated chitosan nanoparticles showed the best property of sustained release and biocompatibility.The membrane had a loose inner layer and a dense outer layer,and the distribution of the nanoparticles was uniform in the inner layer of the membrane.The release of protein from membrane was unidirectional and the membrane displayed good controlled release property.Conclusions The nanoparticles in-loaded one-way release-controlled chitosan membrane presents good one-way sustained release performance.It is potentially useful in delivery system of growth factors.
