CAJ | 학술논문

目的探讨阿糖胞苷(Ara-c)在癌光啉(PSD-007)介导的光动力疗法(PDT)杀伤人早幼粒白血病HL-60细胞中的联合作用.方法实验分为空白对照组、PDT单独作用组(PDT l～4组,为光敏剂剂量(5、7.5μg/ml)和激光能量密度(1.2、2.4 J/cm2)的两两组合)、Ara-c单独作用组(Ara-c A组和Ara-c B组中Ara-c质量浓度分别为0.3 μg/ml和1.2μg/ml)和联合作用组即上述PDT单独作用组和Ara-c单独作用组的两两组合.所有分组分别按3种时序即P24A时序(PDT作用24h后再加入Ara-c共同作用24h)、A24P时序(Ara-c作用24h后进行PDT再共同作用24h)和PA24时序(PDT作用的同时加入Ara-c再共同作用24h)进行处理.采用CCK-8法检测各组细胞活性,用金氏公式分析联合效应,并用流式细胞术检测细胞周期变化.结果小剂量PSD-007介导的PDT和Ara-c联合时,3种时序的联合作用均表现为协同效应;而大剂量PSD-007介导的PDT和Ara-c联合时,P24A和A24P 2种时序的联合作用效应为协同或相加,PA24时序则主要为相加或拮抗.流式细胞仪检测细胞周期变化结果显示,Ara-c和PSD介导的PDT均能引起细胞周期G0/G1期阻滞.结论 Ara-c与PSD-007介导的PDT联合作用对HL-60细胞的杀伤有协同作用,其协同作用与剂量和作用时序相关,小剂量比大剂量协同效果明显,且Ara-c和PDT间隔24h作用比2者同时作用于该细胞的协同效果明显.
목적 탐토아당포감(Ara-c)재암광람(PSD-007)개도적광동력요법(PDT)살상인조유립백혈병HL-60세포중적연합작용.방법 실험분위공백대조조、PDT단독작용조(PDT l～4조,위광민제제량(5、7.5μg/ml)화격광능량밀도(1.2、2.4 J/cm2)적량량조합)、Ara-c단독작용조(Ara-c A조화Ara-c B조중Ara-c질량농도분별위0.3 μg/ml화1.2μg/ml)화연합작용조즉상술PDT단독작용조화Ara-c단독작용조적량량조합.소유분조분별안3충시서즉P24A시서(PDT작용24h후재가입Ara-c공동작용24h)、A24P시서(Ara-c작용24h후진행PDT재공동작용24h)화PA24시서(PDT작용적동시가입Ara-c재공동작용24h)진행처리.채용CCK-8법검측각조세포활성,용금씨공식분석연합효응,병용류식세포술검측세포주기변화.결과 소제량PSD-007개도적PDT화Ara-c연합시,3충시서적연합작용균표현위협동효응;이대제량PSD-007개도적PDT화Ara-c연합시,P24A화A24P 2충시서적연합작용효응위협동혹상가,PA24시서칙주요위상가혹길항.류식세포의검측세포주기변화결과현시,Ara-c화PSD개도적PDT균능인기세포주기G0/G1기조체.결론 Ara-c여PSD-007개도적PDT연합작용대HL-60세포적살상유협동작용,기협동작용여제량화작용시서상관,소제량비대제량협동효과명현,차Ara-c화PDT간격24h작용비2자동시작용우해세포적협동효과명현.
Objective To evaluate the combined effects of photodynamic therapy (PDT) with PSD-007 and cytarabine (Ara-c) on human acute promyelocyte leukemia cell HL-60.Methods The experiments were divided into four groups:control group,PDT-only groups (PDT 1-4 groups:the combination of PSD-007 concentrations (5 μg/ml and 7.5 μg/ml) and the energy density of laser (EDL) (1.2 J/cm2 and 2.4 J/cm2),Ara-c-only groups (Ara-c A group:0.3μg/ml,Ara-c B group:1.2μg/ml) and combination groups (the pair-wise combinations of the PDT doses and Ara-c doses above).All combination groups were treated with three treating methods,including P24A (the cells were treated with PDT for 24 h,and then cocultured with Ara-c for another 24 h),A24P (treated with Ara-c for 24 h before PDT,and then cocultured with PDT for 24 h),and PA24 (treated with the Ara-c and PDT for 24 h).CCK-8 method was used to test the cell viability,and the combined effect was analyzed using King formula.The changes of cell cycles were analyzed using flow cytometry.Results In the case of low-dose PDT,the combination groups showed coordinated effect with all three methods,whereas in the case of high-dose PDT,they showed additive or coordinated effect in P24A and A24P,but it showed antagonistic effect in the schedule of PA24.Cell cycles were inhibited to G0/G1 phase by both PDT and Ara-c.Conclusions Coordinated effects could be found when HL-60 cells were treated with the combination of Ara-c and PDT.The effects depended on the dose of Ara-c and PDT and the operation schedules.The effects at low dose were more obvious than that of high dose,and allowing a 24 h period in between the addition of PDT and Ara-c also promoted the effects.