CAJ | 학술논문

目的研究聚乳酸-羟基乙酸(PLGA)三维支架在骨髓干细胞向胰岛样细胞诱导分化中的作用.方法分别在平面和PLGA三维支架上对小鼠骨髓干细胞向胰岛样细胞进行诱导分化,对2组诱导分化的胰岛样细胞进行形态学观察和功能检测,对照组为小鼠胰岛细胞.结果 2组诱导分化的胰岛样细胞均呈双硫腙(DTZ)染色阳性,胰岛素、C肽双染阳性,胰高血糖素阳性和生长抑素阳性;在高糖刺激下,PLGA三维支架组的胰岛素分泌量较平面组显著提高(P＜0.01).电镜提示诱导分化的胰岛样细胞具有类似β细胞的超微结构且与支架有良好的相容性.结论 PLGA三维支架有益于提高骨髓干细胞诱导分化的胰岛样细胞功能,且与胰岛样细胞具有良好的相容性.
목적 연구취유산-간기을산(PLGA)삼유지가재골수간세포향이도양세포유도분화중적작용.방법 분별재평면화PLGA삼유지가상대소서골수간세포향이도양세포진행유도분화,대2조유도분화적이도양세포진행형태학관찰화공능검측,대조조위소서이도세포.결과 2조유도분화적이도양세포균정쌍류종(DTZ)염색양성,이도소、C태쌍염양성,이고혈당소양성화생장억소양성;재고당자격하,PLGA삼유지가조적이도소분비량교평면조현저제고(P＜0.01).전경제시유도분화적이도양세포구유유사β세포적초미결구차여지가유량호적상용성.결론 PLGA삼유지가유익우제고골수간세포유도분화적이도양세포공능,차여이도양세포구유량호적상용성.
Objective To investigated the potential influences of poly(lactic-co-glycolicacid)(PLGA)scaffold as a platform on the differentiation of mouse bone-marrow stem cells to islet-like cells.Methods Mouse bonemarrow stem cells were grown and differentiated in culture with or without PLGA scaffold,and cell morphology and functions were compared within these groups.Results The PLGA scaffold showed fine biological compatibility.Differentiated islet-like cells were dithizone (DTZ) positive,insulin and C-peptide double positive,glucagon positive and somatostatin positive in both groups.Under electron microscope there were ultrastructures similar to that of islet β cells in cells of both groups.Cells with PLGA scaffold secreted more insulin under high level glucose stimulation (P＜0.01).Conclusions PLGA scaffold was biologically compatible and improved function of the differentiated islet-like cells.