CAJ | 학술논문

目的评估聚吡咯/PLGA可导电复合纳米纤维支架对肝细胞的生物相容性.方法采用静电纺丝及氧化结合法制备聚吡咯/PLGA可导电复合纳米纤维支架,并对其进行电镜表征及电化学检测.采用2步原位胶原酶法分离培养大鼠原代肝细胞.实验组将大鼠肝细胞培养于聚吡咯/PLGA可导电复合纳米纤维支架表面,而对照组设为PLGA培养组及空白对照组;采用MTT法检测各组细胞活性,BCA试剂盒评估各组细胞数量,ELISA法检测白蛋白分泌及尿素合成,同时还测定了各组肿瘤坏死因子-α(TNF-α)及乳酸脱氢酶(LDH)的浓度.结果 MTT实验及BCA检测提示聚吡咯/PLGA组与PLGA组间细胞数量及活性差异无统计学意义,但相比空白对照组其差异均具有统计学意义(P＜0.05).白蛋白分泌及尿素合成方面也有类似表现,聚吡咯/PLGA组与PLGA组间差异无统计学意义,但较空白对照组要好.各组TNF-α及LDH的浓度差异无统计学意义(P＞0.05).结论聚吡咯/PLGA可导电复合纳米纤维支架具有良好的生物相容性,可以作为肝脏组织工程中肝细胞黏附介质的理想材料.
목적 평고취필각/PLGA가도전복합납미섬유지가대간세포적생물상용성.방법 채용정전방사급양화결합법제비취필각/PLGA가도전복합납미섬유지가,병대기진행전경표정급전화학검측.채용2보원위효원매법분리배양대서원대간세포.실험조장대서간세포배양우취필각/PLGA가도전복합납미섬유지가표면,이대조조설위PLGA배양조급공백대조조;채용MTT법검측각조세포활성,BCA시제합평고각조세포수량,ELISA법검측백단백분비급뇨소합성,동시환측정료각조종류배사인자-α(TNF-α)급유산탈경매(LDH)적농도.결과 MTT실험급BCA검측제시취필각/PLGA조여PLGA조간세포수량급활성차이무통계학의의,단상비공백대조조기차이균구유통계학의의(P＜0.05).백단백분비급뇨소합성방면야유유사표현,취필각/PLGA조여PLGA조간차이무통계학의의,단교공백대조조요호.각조TNF-α급LDH적농도차이무통계학의의(P＞0.05).결론 취필각/PLGA가도전복합납미섬유지가구유량호적생물상용성,가이작위간장조직공정중간세포점부개질적이상재료.
Objective To evaluate the biocompatibility of polypyrrole/PLGA conductive nanofiber scaffold on hepatocytes.Methods The polypyrrole/PLGA conductive nanofiber scaffold was designed via electrospinning and oxidative polymerization,and characterized by scanning electron microscope (SEM) and cyclic voltammetry.Rat hepatocytes were harvested by a two-step in situ collagenase perfusion procedure.Rats were divided as polypyrrole/PLGA group,PLGA group and the blank control group.The cytoactivity was detected by MTT assay,cell number was assessed by BCA kit,the albumin secretion and urea synthesis were detected by enzyme-linked immunosorbent assay (ELISA),and the concentration of lactate dehydrogenase (LDH) and tumor necrosis factor-α (TNF-α) was also measured.Results There was no significant difference in the cell number and cytoactivity between polypyrrole/PLGA group and PLGA group (P＞0.05),which were both better than the blank control group (P＜0.05).The urea synthesis and albumin secretion of hepatocytes on polypyrrole/PLGA conductive scaffold had a similar phenomenon.There was no significant difference between the concentration of LDH and TNF-α among the three groups (P＞0.05).Conclusions The polypyrrole/ PLGA conductive nanofiber scaffold shows good biocompatibility,which suggests its potential application as a suitable substratum for hepatocytes culturing in liver tissue engineering.