国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2014年
5期
275-278
,共4页
阚伯红%王一婧%胡先同%高青%范英昌%何颖娜%赵孔银
闞伯紅%王一婧%鬍先同%高青%範英昌%何穎娜%趙孔銀
감백홍%왕일청%호선동%고청%범영창%하영나%조공은
骨髓干细胞%心复康%间质源性细胞因子α
骨髓榦細胞%心複康%間質源性細胞因子α
골수간세포%심복강%간질원성세포인자α
Bone marrow stem cells%Xinfukang%SDF-1α
目的 研究心复康含药血清对骨髓干细胞(BMSCs)中间质源性细胞因子α(SDF-1α)mRNA表达和蛋白分泌的影响.方法 采用全骨髓培养法分离和扩增BMSCs,并用流式细胞仪进行鉴定.以定量PCR(q-PCR)和酶联免疫吸附测定法(ELISA)分别检测含药血清作用后BMSCs中SDF-1α mRNA表达和蛋白分泌的改变情况.结果 经心复康含药血清作用72 h后,SDF-1α mRNA的表达量明显增加,实验组约为对照组的200倍(P＜0.05);SDF-1α蛋白分泌量增加近1倍(P＜0.05),其中实验组为(277.561 1±15.651 8)pg/ml,对照组(153.107 1±14.765 1)pg/ml.结论 全骨髓培养法可获得高纯度的BMSCs,含药血清可明显促进SDF-1αmRNA的表达及其蛋白分泌.
目的 研究心複康含藥血清對骨髓榦細胞(BMSCs)中間質源性細胞因子α(SDF-1α)mRNA錶達和蛋白分泌的影響.方法 採用全骨髓培養法分離和擴增BMSCs,併用流式細胞儀進行鑒定.以定量PCR(q-PCR)和酶聯免疫吸附測定法(ELISA)分彆檢測含藥血清作用後BMSCs中SDF-1α mRNA錶達和蛋白分泌的改變情況.結果 經心複康含藥血清作用72 h後,SDF-1α mRNA的錶達量明顯增加,實驗組約為對照組的200倍(P＜0.05);SDF-1α蛋白分泌量增加近1倍(P＜0.05),其中實驗組為(277.561 1±15.651 8)pg/ml,對照組(153.107 1±14.765 1)pg/ml.結論 全骨髓培養法可穫得高純度的BMSCs,含藥血清可明顯促進SDF-1αmRNA的錶達及其蛋白分泌.
목적 연구심복강함약혈청대골수간세포(BMSCs)중간질원성세포인자α(SDF-1α)mRNA표체화단백분비적영향.방법 채용전골수배양법분리화확증BMSCs,병용류식세포의진행감정.이정량PCR(q-PCR)화매련면역흡부측정법(ELISA)분별검측함약혈청작용후BMSCs중SDF-1α mRNA표체화단백분비적개변정황.결과 경심복강함약혈청작용72 h후,SDF-1α mRNA적표체량명현증가,실험조약위대조조적200배(P＜0.05);SDF-1α단백분비량증가근1배(P＜0.05),기중실험조위(277.561 1±15.651 8)pg/ml,대조조(153.107 1±14.765 1)pg/ml.결론 전골수배양법가획득고순도적BMSCs,함약혈청가명현촉진SDF-1αmRNA적표체급기단백분비.
Objective To clarify the effects of Xinfukang containing-serum on stromal cell-derived factor-1α (SDF-1α) translation and protein secretion of bone marrow stem cells (BMSCs).Methods BMSCs were isolated and amplified using bone marrow culture method,and were identified by flow cytometry.mRNA and protein secretion of SDF-1α were detected by quantitative PCR (q-PCR) and enzyme linked immunosorbent assay (ELISA),respectively.Results The expression of SDF-1α mRNA were significantly increased after 72 h in drug-containing serum,and SDF-1α mRNA in the experimental group was approximately 200 times as that in the control group (P＜0.05).Secretion of SDF-1 α in the experimental group (277.561 1 ± 15.651 8) pg/ml was nearly doubled compared with that in the control group (153.107 1±14.765 1) pg/ml (P＜0.05).Conclusions BMSCs from whole bone marrow adherent culture have high purity,and drug-containing serum can promote BMSCs to express SDF-1 α mRNA and secretion of SDF-1 α.
