国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2014年
5期
283-286,294,封3
,共6页
陈靖京%洪阁%高莉晶%刘天军
陳靖京%洪閣%高莉晶%劉天軍
진정경%홍각%고리정%류천군
新型光敏药物%HepG2细胞%药物吸收量%亚细胞定位%凋亡
新型光敏藥物%HepG2細胞%藥物吸收量%亞細胞定位%凋亡
신형광민약물%HepG2세포%약물흡수량%아세포정위%조망
Novel photosensitizer%HepG2 cell%Photosensitizer uptake%Subcellular localization%Apoptosis
目的 探讨一种新型卟啉类光敏药物对人肝癌HepG2细胞的光动力学治疗(PDT)作用及其机制.方法 实验分为4组:阴性对照组,PDT组,药物组及药物+PDT组.采用Bleaching法研究光敏药物的光稳定性;MTT法检测药物对HepG2细胞的PDT杀伤作用;荧光分光光度计检测光敏药物的细胞吸收量;激光共聚焦显微镜检测药物在癌细胞内定位;Hoechst 333342染色检测PDT后HepG2细胞死亡方式.结果 药物对光照比较稳定;单纯光照及单纯光敏药物自身均对HepG2细胞生长无任何影响(P＞0.05),但药物孵育后行激光照射对HepG2细胞有强烈的PDT杀伤作用(P＜0.05),IC50值为1.21 μmol/;细胞的药物吸收量呈显著的浓度依赖性,浓度至12.5 μmol/L时吸收量达到饱和;光敏药物分布于HepG2细胞溶酶体内;PDT后HepG2细胞死亡方式主要为凋亡.结论 新型光敏药物能够杀伤人肝癌HepG2细胞,其方式主要通过损伤溶酶体启动继发性的细胞凋亡实现的.
目的 探討一種新型卟啉類光敏藥物對人肝癌HepG2細胞的光動力學治療(PDT)作用及其機製.方法 實驗分為4組:陰性對照組,PDT組,藥物組及藥物+PDT組.採用Bleaching法研究光敏藥物的光穩定性;MTT法檢測藥物對HepG2細胞的PDT殺傷作用;熒光分光光度計檢測光敏藥物的細胞吸收量;激光共聚焦顯微鏡檢測藥物在癌細胞內定位;Hoechst 333342染色檢測PDT後HepG2細胞死亡方式.結果 藥物對光照比較穩定;單純光照及單純光敏藥物自身均對HepG2細胞生長無任何影響(P＞0.05),但藥物孵育後行激光照射對HepG2細胞有彊烈的PDT殺傷作用(P＜0.05),IC50值為1.21 μmol/;細胞的藥物吸收量呈顯著的濃度依賴性,濃度至12.5 μmol/L時吸收量達到飽和;光敏藥物分佈于HepG2細胞溶酶體內;PDT後HepG2細胞死亡方式主要為凋亡.結論 新型光敏藥物能夠殺傷人肝癌HepG2細胞,其方式主要通過損傷溶酶體啟動繼髮性的細胞凋亡實現的.
목적 탐토일충신형계람류광민약물대인간암HepG2세포적광동역학치료(PDT)작용급기궤제.방법 실험분위4조:음성대조조,PDT조,약물조급약물+PDT조.채용Bleaching법연구광민약물적광은정성;MTT법검측약물대HepG2세포적PDT살상작용;형광분광광도계검측광민약물적세포흡수량;격광공취초현미경검측약물재암세포내정위;Hoechst 333342염색검측PDT후HepG2세포사망방식.결과 약물대광조비교은정;단순광조급단순광민약물자신균대HepG2세포생장무임하영향(P＞0.05),단약물부육후행격광조사대HepG2세포유강렬적PDT살상작용(P＜0.05),IC50치위1.21 μmol/;세포적약물흡수량정현저적농도의뢰성,농도지12.5 μmol/L시흡수량체도포화;광민약물분포우HepG2세포용매체내;PDT후HepG2세포사망방식주요위조망.결론 신형광민약물능구살상인간암HepG2세포,기방식주요통과손상용매체계동계발성적세포조망실현적.
Objective To study the photodynamic therapy (PDT) mediated by a novel porphyrin-typed photosensitizer on human hepatic carcinoma HepG2 cell and the mechanisms.Methods Experiments were derided into four groups:control group,PDT group,photosensitizer group and photosensitizer+PDT group.The photostability of novel photosensitizer upon repetitive illumination was evaluated by bleaching method,and cell survival rate was determined by MTT assay.Cellular uptake of novel photosensitizer was measured with luminescence spectrometer,and cellular localization ofphotosensitizer was observed by laser scanning confocal microscopy (LSCM).Furthermore,apoptotic cell was detected with Hoechst 333342 staining.Results Novel photosensitizer was stable after repetitive light irradiation,and PDT or photosensitizer alone showed no dark cytotoxicity toward HepG2 cell (P＞0.05),but intense killing was observed in photosensitizer+PDT group (P＜0.05).The IC50 is 1.21 μmol/L.Cellular uptake of novel photosensitizer was concentration-dependent and the highest uptake is at concentration of 12.5 μmol/L.Novel photosensitizer localizes in lysosomes of HepG2 cell,and the death mode of HepG2 cell was mainly apoptosis.Conclusions Novel photosensitizer exerts profound cytotoxic effects on HepG2 cell mainly through the initiation of secondary cell apoptosis by lysosome destruction.
