国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2014年
5期
287-290
,共4页
叶绪英%阴慧娟%王宏%张洪梅%顾立超%刘天军
葉緒英%陰慧娟%王宏%張洪梅%顧立超%劉天軍
협서영%음혜연%왕굉%장홍매%고립초%류천군
光动力疗法%宫颈癌细胞%Hela细胞%癌光啉PSD-007
光動力療法%宮頸癌細胞%Hela細胞%癌光啉PSD-007
광동력요법%궁경암세포%Hela세포%암광람PSD-007
Photodynamic therapy%Human cervical cancer cells%Hela%PSD-007
目的 探讨癌光啉(PSD-007)对人宫颈癌Hela细胞体外光动力杀伤效应及主要影响因素.方法 不同质量浓度(0、3.125、6.25、12.5、25、50、100 μg/ml)的PSD-007与Hela细胞共同孵育2h后,予以不同能量(0、0.6、1.2、2.4、4.8、9.6 J/cm2)635 nm波长的激光照射,以相同剂量光照和光敏剂剂量的人乳腺癌细胞系MCF-7光动力杀伤作用做对比,通过噻唑蓝(MTT)比色法测定细胞的光密度(OD)值及存活率;质量浓度为12.5 μg/ml的PSD-007与细胞共同孵育2h后,以不同能量(1.2、2.4、4.8 J/cm2)的激光照射,流式细胞术(FCM)分析细胞周期及凋亡率.结果 与空白对照组相比,单纯光敏剂PSD-007在＞25 μg/ml质量浓度下影响Hela细胞存活率,光动力疗法(PDT)对Hela细胞有更明显的杀伤效应,并且随着光敏剂质量浓度增加和光照能量密度增大,对细胞的杀伤效果逐渐增强.当光敏剂质量浓度＞12.5 μg/ml,光照能量＞4.8 J/cm2时,各组间细胞存活率的差异无统计学意义(P＞0.05).FCM分析发现,PDT于G0/G1期阻断Hela细胞生长,凋亡诱导作用呈时间依赖性.结论 光敏剂PSD-007自身对体外人宫颈癌细胞系Hela细胞具有生长抑制作用,PSD-007介导的光动力杀伤效应更为显著,较人乳腺癌-7(MCF-7)细胞的PSD-007光动力作用有更低的使用剂量和光照剂量.
目的 探討癌光啉(PSD-007)對人宮頸癌Hela細胞體外光動力殺傷效應及主要影響因素.方法 不同質量濃度(0、3.125、6.25、12.5、25、50、100 μg/ml)的PSD-007與Hela細胞共同孵育2h後,予以不同能量(0、0.6、1.2、2.4、4.8、9.6 J/cm2)635 nm波長的激光照射,以相同劑量光照和光敏劑劑量的人乳腺癌細胞繫MCF-7光動力殺傷作用做對比,通過噻唑藍(MTT)比色法測定細胞的光密度(OD)值及存活率;質量濃度為12.5 μg/ml的PSD-007與細胞共同孵育2h後,以不同能量(1.2、2.4、4.8 J/cm2)的激光照射,流式細胞術(FCM)分析細胞週期及凋亡率.結果 與空白對照組相比,單純光敏劑PSD-007在＞25 μg/ml質量濃度下影響Hela細胞存活率,光動力療法(PDT)對Hela細胞有更明顯的殺傷效應,併且隨著光敏劑質量濃度增加和光照能量密度增大,對細胞的殺傷效果逐漸增彊.噹光敏劑質量濃度＞12.5 μg/ml,光照能量＞4.8 J/cm2時,各組間細胞存活率的差異無統計學意義(P＞0.05).FCM分析髮現,PDT于G0/G1期阻斷Hela細胞生長,凋亡誘導作用呈時間依賴性.結論 光敏劑PSD-007自身對體外人宮頸癌細胞繫Hela細胞具有生長抑製作用,PSD-007介導的光動力殺傷效應更為顯著,較人乳腺癌-7(MCF-7)細胞的PSD-007光動力作用有更低的使用劑量和光照劑量.
목적 탐토암광람(PSD-007)대인궁경암Hela세포체외광동력살상효응급주요영향인소.방법 불동질량농도(0、3.125、6.25、12.5、25、50、100 μg/ml)적PSD-007여Hela세포공동부육2h후,여이불동능량(0、0.6、1.2、2.4、4.8、9.6 J/cm2)635 nm파장적격광조사,이상동제량광조화광민제제량적인유선암세포계MCF-7광동력살상작용주대비,통과새서람(MTT)비색법측정세포적광밀도(OD)치급존활솔;질량농도위12.5 μg/ml적PSD-007여세포공동부육2h후,이불동능량(1.2、2.4、4.8 J/cm2)적격광조사,류식세포술(FCM)분석세포주기급조망솔.결과 여공백대조조상비,단순광민제PSD-007재＞25 μg/ml질량농도하영향Hela세포존활솔,광동력요법(PDT)대Hela세포유경명현적살상효응,병차수착광민제질량농도증가화광조능량밀도증대,대세포적살상효과축점증강.당광민제질량농도＞12.5 μg/ml,광조능량＞4.8 J/cm2시,각조간세포존활솔적차이무통계학의의(P＞0.05).FCM분석발현,PDT우G0/G1기조단Hela세포생장,조망유도작용정시간의뢰성.결론 광민제PSD-007자신대체외인궁경암세포계Hela세포구유생장억제작용,PSD-007개도적광동력살상효응경위현저,교인유선암-7(MCF-7)세포적PSD-007광동력작용유경저적사용제량화광조제량.
Objective To investigate the photochemotherapeutic effect and the main affecting factors of PSD-007 on human cervical cancer Hela in vitro.Methods Hela cells were treated with different concentrations of PSD-007 (0,3.125,6.25,12.5,25,50,100 μg/ml) for 2 h under the influence of low-level laser (635 nm) therapy at different doses (0,0.6,1.2,2.4,4.8,9.6 J/cm2).Then the OD values and survival rates of Hela cells were measured by MTT assay compared with breast cancer cells MCF-7 in same treatment.Hela cells were treated with 12.5 μg/ml of PSD-007 for 2 h and were treated with different intensities of laser (1.2,2.4,4.8 J/cm2).The cellular apoptosis rate and cell cycle phase distribution of Hela were measured by a flow cytometry (FCM).Results Survival rates of Hela cells declined with more than 25 μg/ml of PSD-007 only,and significant difference in the inhibitory between the PDT group and control group was observed (P＜0.05).The survival rates of Hela after PDT was decreased by the concentration of sensitizer and dose of laser.There were no significant differences of cell survival rates among the groups with concentrations more than 12.5 μg/ml and laser energy density more than 4.8 J/cm2.The FCM assay showed a G0/G1 cell cycle arrest in a time-dependent manner.Conclusions PSD-007 has a photodynamic effect on Hela in vitro.Photodynamic effect of PSD-007 was more significant in Hela than MCF-7.Less photosensitizer and laser energy density were needed.
