国际输血及血液学杂志
國際輸血及血液學雜誌
국제수혈급혈액학잡지
INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
2013年
3期
210-212
,共3页
卢瑾%周健欣%余晋林%朱业华
盧瑾%週健訢%餘晉林%硃業華
로근%주건흔%여진림%주업화
无偿献血者%核酸检测%酶联免疫吸附试验
無償獻血者%覈痠檢測%酶聯免疫吸附試驗
무상헌혈자%핵산검측%매련면역흡부시험
volunteer blood donors%nucleic acid test%enzyme-linked immunosorbent assay
目的 通过分析无偿献血者全血标本的核酸检测(NAT)和血清学检测的结果,探讨NAT在筛查乙型肝炎病毒(HBV),丙型肝炎病毒(HCV)和人类免疫缺陷病毒(HIV)“窗口期”感染者或者隐匿性肝炎的患者中的作用.方法 选择2011年7月至12月于佛山市中心血站采集的无偿献血者全血样本13 126份作为研究对象.对无偿献血者全血标本同时进行血清学检测和NAT.使用诺华诊断血液筛查系统对标本进行单人份NAT.对于NAT联检和鉴别试验阳性而血清学试验阴性的献血者,进一步检测乙型肝炎血清标志物5项指标.结果 13 126份标本中,NAT联检试验阳性为128例(0.98％),其中95例为酶联免疫吸附试验(ELISA)双试剂阳性,4例为ELISA单试剂阳性,29例为ELISA阴性.对NAT联检试验和鉴别试验结果皆呈阳性的8例献血者做进一步追踪,6例行乙型肝炎血清标志物5项指标检测的结果显示,1例抗-HBs和抗-HBc呈阳性,3例仅抗-HBc呈阳性,2例呈全阴性.结论 NAT作为一种检测原理完全与ELISA不同的方法,能够在一定程度上弥补ELISA方法的局限性,有效缩短“窗口期”.NAT与ELISA两种检测方法能够互为补充,共同降低经输血途径传播病毒的风险.
目的 通過分析無償獻血者全血標本的覈痠檢測(NAT)和血清學檢測的結果,探討NAT在篩查乙型肝炎病毒(HBV),丙型肝炎病毒(HCV)和人類免疫缺陷病毒(HIV)“窗口期”感染者或者隱匿性肝炎的患者中的作用.方法 選擇2011年7月至12月于彿山市中心血站採集的無償獻血者全血樣本13 126份作為研究對象.對無償獻血者全血標本同時進行血清學檢測和NAT.使用諾華診斷血液篩查繫統對標本進行單人份NAT.對于NAT聯檢和鑒彆試驗暘性而血清學試驗陰性的獻血者,進一步檢測乙型肝炎血清標誌物5項指標.結果 13 126份標本中,NAT聯檢試驗暘性為128例(0.98％),其中95例為酶聯免疫吸附試驗(ELISA)雙試劑暘性,4例為ELISA單試劑暘性,29例為ELISA陰性.對NAT聯檢試驗和鑒彆試驗結果皆呈暘性的8例獻血者做進一步追蹤,6例行乙型肝炎血清標誌物5項指標檢測的結果顯示,1例抗-HBs和抗-HBc呈暘性,3例僅抗-HBc呈暘性,2例呈全陰性.結論 NAT作為一種檢測原理完全與ELISA不同的方法,能夠在一定程度上瀰補ELISA方法的跼限性,有效縮短“窗口期”.NAT與ELISA兩種檢測方法能夠互為補充,共同降低經輸血途徑傳播病毒的風險.
목적 통과분석무상헌혈자전혈표본적핵산검측(NAT)화혈청학검측적결과,탐토NAT재사사을형간염병독(HBV),병형간염병독(HCV)화인류면역결함병독(HIV)“창구기”감염자혹자은닉성간염적환자중적작용.방법 선택2011년7월지12월우불산시중심혈참채집적무상헌혈자전혈양본13 126빈작위연구대상.대무상헌혈자전혈표본동시진행혈청학검측화NAT.사용낙화진단혈액사사계통대표본진행단인빈NAT.대우NAT련검화감별시험양성이혈청학시험음성적헌혈자,진일보검측을형간염혈청표지물5항지표.결과 13 126빈표본중,NAT련검시험양성위128례(0.98％),기중95례위매련면역흡부시험(ELISA)쌍시제양성,4례위ELISA단시제양성,29례위ELISA음성.대NAT련검시험화감별시험결과개정양성적8례헌혈자주진일보추종,6례행을형간염혈청표지물5항지표검측적결과현시,1례항-HBs화항-HBc정양성,3례부항-HBc정양성,2례정전음성.결론 NAT작위일충검측원리완전여ELISA불동적방법,능구재일정정도상미보ELISA방법적국한성,유효축단“창구기”.NAT여ELISA량충검측방법능구호위보충,공동강저경수혈도경전파병독적풍험.
Objective To explore the role of nucleic acid test (NAT) in screening the "window period" or occult hepatitis B virus infection (OBI) blood donators by comparing the results of NAT and enzyme-linked immunosorbent assay (ELISA) of the blood samples.Methods Form July to December 2011,A total of 13 126 blood samples from volunteer blood donors in Foshan blood center were included in this study.All of this samples were tested by NAT using procleix TIGRIS system and ELISA simultaneously.Five markers of hepatitis B virus (HBV) of donators who were positive tested by ProcleixUltrio and Discrimination but negative tested by ELISA were detected.Results Among all the samples,128 (0.98％) cases were NAT positive.Among the 128 cases,95 cases were detected positive by two kinds of ELISA reagents,4 cases were detected positive by only one kind of ELISA reagent,and another 29 cases were detected negative by two kinds of ELISA reagents.8 cases results were traced,and 6 of the 8 donators were detected the five markers of HBV,4 of them were positive for anti-HBc.Conclusions As a new detect method different from ELISA,NAT is used to cut down the "window periods" of virus detection.The blood screening strategy using both NAT and ELISA could improve the safety of blood transfusion.
