CAJ | 학술논문

目的探讨人类白细胞抗原(HLA)-DPA1和-DPB1基因多态性与中国南方汉族人群鼻咽癌的相关性.方法选择2012年2月至7月于广西梧州市红十字会医院及深圳市武警医院经病理学检查确诊为鼻咽癌(NPC)病变的南方汉族患者74例为研究对象,纳入研究组；计算机随机选择同期于本中心献血的南方汉族健康志愿捐者160例,纳入对照组(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该伦理会批准,分组征得受试对象本人的知情同意,并与之签订临床研究知情同意书).采用测序分型方法对研究组和对照组的血液样本进行HLA-DPA1和-DPB1基因第2外显子双向测序,用Assign 3.5 SBT分析软件判定等位基因型.采用Wolf法进行x2检验并计算P值和相对危险度(RR),比较研究组和对照组HLA-DPA1和-DPB1等位基因频率的差异.结果研究组和对照组中,均检出4个DPA1等位基因,即DPAl* 01:03,02:01,02:02和04:01.作为多态性丰富的HLA-DPB1位点,研究组中共检出14个等位基因,对照组中共检出18个等位基因.研究组与对照组的HLA-DPA1和-DPB1等位基因的基因检出率比较,差异均无统计学意义(x2＜3.84,P＞0.05).结论本文的结果初步显示中国南方汉族人群中HLA-DPA1和-DPB1基因多态性与NPC无相关性.
목적 탐토인류백세포항원(HLA)-DPA1화-DPB1기인다태성여중국남방한족인군비인암적상관성.방법 선택2012년2월지7월우엄서오주시홍십자회의원급심수시무경의원경병이학검사학진위비인암(NPC)병변적남방한족환자74례위연구대상,납입연구조；계산궤수궤선택동기우본중심헌혈적남방한족건강지원연자160례,납입대조조(본연구준순적정서부합본원인체시험위원회소제정적윤리학표준,득도해윤리회비준,분조정득수시대상본인적지정동의,병여지첨정림상연구지정동의서).채용측서분형방법대연구조화대조조적혈액양본진행HLA-DPA1화-DPB1기인제2외현자쌍향측서,용Assign 3.5 SBT분석연건판정등위기인형.채용Wolf법진행x2검험병계산P치화상대위험도(RR),비교연구조화대조조HLA-DPA1화-DPB1등위기인빈솔적차이.결과 연구조화대조조중,균검출4개DPA1등위기인,즉DPAl* 01:03,02:01,02:02화04:01.작위다태성봉부적HLA-DPB1위점,연구조중공검출14개등위기인,대조조중공검출18개등위기인.연구조여대조조적HLA-DPA1화-DPB1등위기인적기인검출솔비교,차이균무통계학의의(x2＜3.84,P＞0.05).결론 본문적결과초보현시중국남방한족인군중HLA-DPA1화-DPB1기인다태성여NPC무상관성.
Objective To study the correlation of HLA-DPA1 and-DPB1 polymorphisms with nasopharyngeal carcinoma (NPC) in the southern Chinese Han population.Methods From February to July 2012,a total of 74 patients who diagnosed as NPC by pathological examination in Guangxi Wuzhou Red Cross Hospital and Shenzhen Armed Police Hospital were included in this study as study group.And during the same period,a total of 160 healthy donors who were selected by computer randomly in Shenzhen blood center were included in this study as control group (The study protocol was approved by the Ethical Review Board of Investigation in Human Being of our hospital).Both the study group and control group were from southern Chinese Han.All the samples were subjected to sequencing-based typing (PCR-SBT) at exon 2 of HLA-DPA1 and-DPB1 genes in both directions.The allelic frequencies of HLA-DPA1 and-DPB1 loci for the NPC patients were compared with control group by x2 test,the P value and relative risk (RR) value were calculated according to the Wolf method.Results A total of 4 HLA-DPA1 alleles (DPA1 * 01:03,02:01,02:02 and 04:01) were identified in both study group and control group.As for the highly polymorphic HLA-DPB1 locus,14 HLA-DPB1 alleles in the NPC patient group and 18 DPB1 alleles in the healthy controls were identified.The allele frequencies for both HLA-DPA1 and-DPB1 loci showed no significant differences between study group and control group (x2＜3.84,P＞0.05).Conclusions The present study implied that the polymorphisms of HLA-DPA1 and-DPB1 showed no independent association with NPC in the southern Chinese Han individuals.