花生四烯乙醇胺对体外保存血小板影响的初步研究
화생사희을순알대체외보존혈소판영향적초보연구
Effects of N-Arachidonoylethanolamine on the Quality of Storage Platelets
  • 万方数据
    国际输血及血液学杂志 국제수혈급혈액학잡지
    INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
    2013年 5期 389-394 ,共6页

    刘金玲%庄云龙%杨伦%张毅%乔文本%徐群%赵翠云%周娟%于媛

    류금령%장운룡%양륜%장의%교문본%서군%조취운%주연%우원

    内源性大麻酚类%血小板%血液保存 內源性大痳酚類%血小闆%血液保存
    내원성대마분류%혈소판%혈액보존
    endocannabinoids%blood platelet%blood preservation
    目的 探讨花生四烯乙醇胺(ANA)对体外(22±2)℃水平振荡保存血小板(PLT)的影响.方法 选择2011年11月7日至2011年11月25日,于山东省血液中心自愿捐献PLT的20例献血者作为研究对象.采用Amicus血细胞分离机采集研究对象的PLT,PLT产品摇匀后置于PLT专用保存袋内.在PLT样品中分别加入终浓度为0.1,0.5,1,5,10和50 μmol/L的ANA,相应的对照组中不添加ANA.将PLT样品置于(22±2)℃水平振荡的振荡仪保存.于保存的第7天取样,用噻唑蓝比色法分析PLT的存活率,筛选最佳的ANA浓度.分别于PLT保存袋中取PLT样品50 mL,平均分成2份,其中一份加入最佳浓度的ANA溶液作为实验组(n=20),另一份不加ANA溶液作为对照组(n=20).两组分别于保存的第1,3,5,7,9和11天取样,分析PLT计数、平均PLT体积(MPV)、PLT分布宽度(PDW)、PLT磷脂酰丝氨酸(PS)膜外表达以及可溶性P-选择素含量.结果 加入终浓度为0.5 μmol/L的ANA使PLT存活率最高,约为对照组的1.7倍,两组相比差异具有统计学意义(t=14.32,P＜0.05).于(22±2)℃保存的第1,3,5,7,9和11天,实验组和对照组的PLT计数均呈下降趋势,至保存的第11天时实验组PLT计数显著高于对照组,两组比较,差异有统计学意义(P＜0.05).保存期间实验组和对照组的MPV和PDW均呈增加趋势,至保存的第11天时,实验组MPV和PDW均显著低于对照组[(8.1±0.6)fL比(8.9±0.5)fL;(16.4±0.5)％比(17.0±0.7)％],差异均有统计学意义(P＜0.05).保存的第7,9和11天时,实验组PS表达阳性率显著低于对照组[(8.29±1.44)％比14.24±2.47％;(13.1±1.81)％比(25.85±2.04)％；(21.18±1.78％)比(40.6±1.95)％],两组比较,差异均有统计学意义(P＜0.05).保存第7,9和11天时,实验组可溶性P-选择素含量显著低于对照组[(75.08±6.35)ng/mL比(90.37±8.91)ng/mL;(81.58±6.09)ng/mL比(117.56±7.98)ng/mL;(103.55±7.91)ng/mL比(144.7±7.76)ng/mL],两组比较,差异均有统计学意义(P＜0.05).结论 0.5 μmol/L ANA对PLT的保存具有较好的保护作用,其在一定程度上可以减轻PLT贮存损伤.
    목적 탐토화생사희을순알(ANA)대체외(22±2)℃수평진탕보존혈소판(PLT)적영향.방법 선택2011년11월7일지2011년11월25일,우산동성혈액중심자원연헌PLT적20례헌혈자작위연구대상.채용Amicus혈세포분리궤채집연구대상적PLT,PLT산품요균후치우PLT전용보존대내.재PLT양품중분별가입종농도위0.1,0.5,1,5,10화50 μmol/L적ANA,상응적대조조중불첨가ANA.장PLT양품치우(22±2)℃수평진탕적진탕의보존.우보존적제7천취양,용새서람비색법분석PLT적존활솔,사선최가적ANA농도.분별우PLT보존대중취PLT양품50 mL,평균분성2빈,기중일빈가입최가농도적ANA용액작위실험조(n=20),령일빈불가ANA용액작위대조조(n=20).량조분별우보존적제1,3,5,7,9화11천취양,분석PLT계수、평균PLT체적(MPV)、PLT분포관도(PDW)、PLT린지선사안산(PS)막외표체이급가용성P-선택소함량.결과 가입종농도위0.5 μmol/L적ANA사PLT존활솔최고,약위대조조적1.7배,량조상비차이구유통계학의의(t=14.32,P＜0.05).우(22±2)℃보존적제1,3,5,7,9화11천,실험조화대조조적PLT계수균정하강추세,지보존적제11천시실험조PLT계수현저고우대조조,량조비교,차이유통계학의의(P＜0.05).보존기간실험조화대조조적MPV화PDW균정증가추세,지보존적제11천시,실험조MPV화PDW균현저저우대조조[(8.1±0.6)fL비(8.9±0.5)fL;(16.4±0.5)％비(17.0±0.7)％],차이균유통계학의의(P＜0.05).보존적제7,9화11천시,실험조PS표체양성솔현저저우대조조[(8.29±1.44)％비14.24±2.47％;(13.1±1.81)％비(25.85±2.04)％；(21.18±1.78％)비(40.6±1.95)％],량조비교,차이균유통계학의의(P＜0.05).보존제7,9화11천시,실험조가용성P-선택소함량현저저우대조조[(75.08±6.35)ng/mL비(90.37±8.91)ng/mL;(81.58±6.09)ng/mL비(117.56±7.98)ng/mL;(103.55±7.91)ng/mL비(144.7±7.76)ng/mL],량조비교,차이균유통계학의의(P＜0.05).결론 0.5 μmol/L ANA대PLT적보존구유교호적보호작용,기재일정정도상가이감경PLT저존손상.
    Objective To evaluate the impact of N-Arachidonoylethanolamine(ANA)on the quality of platelets(PLT)stored at(22±2)℃.Methods From November 7th to 25th 2011,a total of 20 volunteers who donated PLT in Blood Center of Shandong Province were included into this study.Apheresis PLTs were collected through the Amicus instrument.Different working concentrations of ANA(0.1,0.5,1,5,10 and 50 μmol/L)were added to the PLTs samples,respectively,as the experimental group,another corresponding parts with no ANA as the controls,respectively.All the PLT samples were stored at(22±2)℃ for 7 days,then were evaluated the viability by measuring the mitochondrial reduction with methyl thiazolyl tetrazolium colorimetric for detecting the most effective concentration of ANA.Samples(50 mL/each)were taken and split into two storage parts(25 mL/each).The most effective concentration of ANA was added one part as the experimental group(n=20),the other with no ANA as the control group(n=20).These PLT samples were stored on a flat-bed shaker at(22±2)℃.PLT count,mean PLT volume (MPV),PLT distribution width(PDW),phosphatidyl serine(PS)and soluble P-selectin of the two groups were detected on the 1st,3rd,5th,7th,9th and 11th day of storage.Results The most effective final concentration of ANA was 0.5 μmol/L,which showed significant increasing PLTs survival(about 1.7 fold more than controls)(t=14.32,P＜0.05).During 11 days of storage,the PLT counts were not changed significantly until on the 11th day of storage between the experimental group and control group.Moreover,the MPV and PDW were decreased significantly in the experimental group than those in the control group on the 11th day of storage(P＜0.05),respectively.The rates of PLT PS positive were on the rise during the storage period:the experimental group were from(8.29 ±1.44)％ to(21.18 ± 1.78)％ while the control group were from(14.24±2.47)％ to(40.6± 1.95)％,with significant differences between the two groups (P＜0.05)on the 7th,9th and 11th day of storage,respectively.Soluble P-selectin contents of the experimental group on the 7th,9th and 11th day of storage were(75.08± 6.35)ng/mL,(81.58±6.09)ng/mL and(103.55±7.91)ng/mL,while those in the control group were(90.37±8.91)ng/mL,(117.56 ± 9.98)ng/mL and(144.7 ± 7.76)ng/mL,respectively,with significant differences between the two groups(P＜0.05).Conclusions 0.5 μmol/L ANA had a good protective effect on PLTs,and could potentially alleviate PLT storage lesions to some extent.
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