CAJ | 학술논문

目的评价不同剂量血红蛋白类携氧载体(HBOC)对大鼠肺动脉平滑肌细胞大电导钙离子激活钾离子通道(BKCa)电流的影响.方法采用二步酶消化法急性分离Sprague Dawley(SD)大鼠肺动脉平滑肌细胞.BKCa电流采用四氨基吡啶(4-AP)进行分离并使用全细胞电压钳记录.选取急性分离后状态良好的大鼠肺动脉平滑肌细胞30个,根据计算机生成的随机数将其分为5组,每组6个平滑肌细胞,分别给予血红蛋白含量(kg/L)为0.25×10-2,0.50×10-2,1.00×10-2,2.00×10-2,3.00×10-2的HBOC处理.观察不同剂量HBOC对BKCa电流的影响,并与给予HBOC前的BKCa电流相比较(实验过程中对动物的处置符合动物伦理学标准).结果经血红蛋白含量为0.25×10-2 kg/L的HBOC处理后,BKCa电流轻度增加,BKCa电流值与基础值比较,增加3.90％;分别给予血红蛋白含量(kg/L)为0.50×10-2,1.00×10-2,2.00×10-2,3.00×10-2的HBOC处理后,BKCa电流呈不同程度下降,BKCa电流值与基础值比较,分别下降8.04％,28.61％,46.53％和65.97％,HBOC对BKCa电流的抑制作用呈剂量依赖性(b=0.931 6,P=0.007).结论 HBOC呈剂量依赖性抑制大鼠肺动脉平滑肌细胞BKCa电流.BKCa电流的下降可能是HBOC导致血管收缩的原因.
목적 평개불동제량혈홍단백류휴양재체(HBOC)대대서폐동맥평활기세포대전도개리자격활갑리자통도(BKCa)전류적영향.방법 채용이보매소화법급성분리Sprague Dawley(SD)대서폐동맥평활기세포.BKCa전류채용사안기필정(4-AP)진행분리병사용전세포전압겸기록.선취급성분리후상태량호적대서폐동맥평활기세포30개,근거계산궤생성적수궤수장기분위5조,매조6개평활기세포,분별급여혈홍단백함량(kg/L)위0.25×10-2,0.50×10-2,1.00×10-2,2.00×10-2,3.00×10-2적HBOC처리.관찰불동제량HBOC대BKCa전류적영향,병여급여HBOC전적BKCa전류상비교(실험과정중대동물적처치부합동물윤리학표준).결과 경혈홍단백함량위0.25×10-2 kg/L적HBOC처리후,BKCa전류경도증가,BKCa전류치여기출치비교,증가3.90％;분별급여혈홍단백함량(kg/L)위0.50×10-2,1.00×10-2,2.00×10-2,3.00×10-2적HBOC처리후,BKCa전류정불동정도하강,BKCa전류치여기출치비교,분별하강8.04％,28.61％,46.53％화65.97％,HBOC대BKCa전류적억제작용정제량의뢰성(b=0.931 6,P=0.007).결론 HBOC정제량의뢰성억제대서폐동맥평활기세포BKCa전류.BKCa전류적하강가능시HBOC도치혈관수축적원인.
Objective To evaluate the effect of different doses of hemoglobin-based oxygen carriers (HBOC) on the large conductance calcium-activated potassium channels (BKCa) currents of rat pulmonary artery smooth muscle cells.Methods Vascular smooth muscle cells were acutely isolated from rat pulmonary arteries with two steps of enzymatic digestion.BKCa currents were separated with 4-aminopynidine (4-AP),and the effects of HBOC on BKCa currents were evaluated by the whole-cell patch-clamp technique.Thirty rat pulmonary artery smooth muscle cells which were in good condition were selected into this study.They were divided into five groups according to the computer-generated random numbers and six cells each group.The cells in each group were randomly given one of five HBOC concentrations (kg/L)which were 0.25 ×10-2,0.50×10-2,1.00×10-2,2.00×10 2,3.00×10-2.The effects of HBOC on amplitude of BKCa currents were recorded and were compared with the BKCa currents before giving HBOC.All experiments were carried out in accordance with relevant regulatory standards for animal ethics.Results We find that acute addition of 0.25 × 10-2 kg/L HBOC into the bath solution could make the BKCa currents increased 3.90％.While the HBOC concentrations (kg/L) were 0.50× 10-2,1.00× 10-2,2.00×10 2 and 3.00 × 10-2,the BKCa currents were significantly inhibited in a dose-dependent manner (b =0.931 6,P =0.007),with inhibition of 8.04％,28.61％,46.53％ and 65.97％ respectively.Conclusions HBOC could inhibit the BKCa currents of rat pulmonary artery smooth muscle cells in a dosedependent manner.And the inhibition may contribute to HBOC-induced vasoconstriction.