国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2013年
9期
599-604
,共6页
张荣君%章佳新%符德元%邵稳喜%石静%章幂
張榮君%章佳新%符德元%邵穩喜%石靜%章冪
장영군%장가신%부덕원%소은희%석정%장멱
乳腺肿瘤%蛋白质类%SOX7%β-catenin%cyclin D1%免疫组织化学%病理学,临床
乳腺腫瘤%蛋白質類%SOX7%β-catenin%cyclin D1%免疫組織化學%病理學,臨床
유선종류%단백질류%SOX7%β-catenin%cyclin D1%면역조직화학%병이학,림상
Breast neoplasms%Proteins%SOX7%β-catenin%cyclin D1%Immunohistochemistry%Pathology,clinical
目的 观察SOX7、β-catenin和cyclin D1蛋白在浸润性乳腺癌与乳腺良性增生病组织中的表达,探讨它们与浸润性乳腺癌临床病理学特征之间的关系,为浸润性乳腺癌的治疗、预后提供有价值的生物学标志.方法 应用免疫组化SP法分别检测50例浸润性乳腺癌与30例乳腺良性增生病中SOX7、β-cate-nin和cyclin D1蛋白的表达情况,分析SOX7、β-catenin和cyclin D1蛋白的表达与浸润性乳腺癌患者的发病年龄、肿瘤大小、腋窝淋巴结转移、组织学分级、病理学分期(pTNM)、雌激素受体(ER)、孕激素受体(PR)、人类表皮生长因子受体2(Her-2)和危险度分组之间的关系.结果 在50例浸润性乳腺癌组织中,SOX7和cyclin D1蛋白的阳性表达率分别为42％(21/50)和70％(35/50),β-catenin蛋白的异常表达率为70％(35/50).在浸润性乳腺癌中,SOX7蛋白的阳性率明显低于乳腺良性增生病的70％ (21/30) (P =0.021＜0.05),两组间差异有统计学意义；β-catenin蛋白的异常表达率和cyclin D1蛋白的阳性表达率均高于乳腺良性增生病的43.3％ (13/30) (P =0.033 ＜0.05)和20％ (6/30) (P =0.000＜ 0.001),两组间差异均有统计学意义.在50例浸润性乳腺癌中,SOX7和β-catenin蛋白同时阳性表达12例,同时阴性表达5例；SOX7和cyclinD1蛋白同时阳性表达11例,同时阴性表达5例；β-catenin和cyclinD1蛋白同时阳性表达28例,同时阴性表达8例.经Spearman相关分析,SOX7蛋白的阳性表达分别与p-catenin的异常表达和cyclin D1的阳性表达呈负相关关系(r=-0.282,P=0.046 ＜0.05;r =-0.327,P =0.020 ＜0.05),β-catcnin的异常表达与cyclin D1的阳性表达呈正相关关系(r=0.333,P=0.018 ＜0.05).在浸润性乳腺癌中,SOX7蛋白的表达在发病年龄、腋窝淋巴结转移、组织学分级、pTNM分期、ER、PR、Her-2和危险度分组间差异有统计学意义(P＜0.05),而在肿瘤直径分组间比较差异无统计学意义(P ＞0.05)；β-catenin蛋白的异常表达和cyclin D1蛋白的阳性表达在肿瘤直径、腋窝淋巴结转移和pTNM分期分组间差异有统计学意义(P＜0.05),而在发病年龄、组织学分级、ER、PR、Her-2和危险度分组间比较差异无统计学意义(P＞0.05).结论 SOX7、β-cate-nin和cyclinD1蛋白在大多数浸润性乳腺癌组织中表达异常,且三者在浸润性乳腺癌发生、发展过程中可能通过同一通路发挥调节作用.SOX7、β-catenin和cyclin D1蛋白的表达与浸润性乳腺癌的临床病理学特征之间存在一定的相关性,检测三者的表达情况可成为临床上判断浸润性乳腺癌的恶性程度及预后的重要参考指标.
目的 觀察SOX7、β-catenin和cyclin D1蛋白在浸潤性乳腺癌與乳腺良性增生病組織中的錶達,探討它們與浸潤性乳腺癌臨床病理學特徵之間的關繫,為浸潤性乳腺癌的治療、預後提供有價值的生物學標誌.方法 應用免疫組化SP法分彆檢測50例浸潤性乳腺癌與30例乳腺良性增生病中SOX7、β-cate-nin和cyclin D1蛋白的錶達情況,分析SOX7、β-catenin和cyclin D1蛋白的錶達與浸潤性乳腺癌患者的髮病年齡、腫瘤大小、腋窩淋巴結轉移、組織學分級、病理學分期(pTNM)、雌激素受體(ER)、孕激素受體(PR)、人類錶皮生長因子受體2(Her-2)和危險度分組之間的關繫.結果 在50例浸潤性乳腺癌組織中,SOX7和cyclin D1蛋白的暘性錶達率分彆為42％(21/50)和70％(35/50),β-catenin蛋白的異常錶達率為70％(35/50).在浸潤性乳腺癌中,SOX7蛋白的暘性率明顯低于乳腺良性增生病的70％ (21/30) (P =0.021＜0.05),兩組間差異有統計學意義；β-catenin蛋白的異常錶達率和cyclin D1蛋白的暘性錶達率均高于乳腺良性增生病的43.3％ (13/30) (P =0.033 ＜0.05)和20％ (6/30) (P =0.000＜ 0.001),兩組間差異均有統計學意義.在50例浸潤性乳腺癌中,SOX7和β-catenin蛋白同時暘性錶達12例,同時陰性錶達5例；SOX7和cyclinD1蛋白同時暘性錶達11例,同時陰性錶達5例；β-catenin和cyclinD1蛋白同時暘性錶達28例,同時陰性錶達8例.經Spearman相關分析,SOX7蛋白的暘性錶達分彆與p-catenin的異常錶達和cyclin D1的暘性錶達呈負相關關繫(r=-0.282,P=0.046 ＜0.05;r =-0.327,P =0.020 ＜0.05),β-catcnin的異常錶達與cyclin D1的暘性錶達呈正相關關繫(r=0.333,P=0.018 ＜0.05).在浸潤性乳腺癌中,SOX7蛋白的錶達在髮病年齡、腋窩淋巴結轉移、組織學分級、pTNM分期、ER、PR、Her-2和危險度分組間差異有統計學意義(P＜0.05),而在腫瘤直徑分組間比較差異無統計學意義(P ＞0.05)；β-catenin蛋白的異常錶達和cyclin D1蛋白的暘性錶達在腫瘤直徑、腋窩淋巴結轉移和pTNM分期分組間差異有統計學意義(P＜0.05),而在髮病年齡、組織學分級、ER、PR、Her-2和危險度分組間比較差異無統計學意義(P＞0.05).結論 SOX7、β-cate-nin和cyclinD1蛋白在大多數浸潤性乳腺癌組織中錶達異常,且三者在浸潤性乳腺癌髮生、髮展過程中可能通過同一通路髮揮調節作用.SOX7、β-catenin和cyclin D1蛋白的錶達與浸潤性乳腺癌的臨床病理學特徵之間存在一定的相關性,檢測三者的錶達情況可成為臨床上判斷浸潤性乳腺癌的噁性程度及預後的重要參攷指標.
목적 관찰SOX7、β-catenin화cyclin D1단백재침윤성유선암여유선량성증생병조직중적표체,탐토타문여침윤성유선암림상병이학특정지간적관계,위침윤성유선암적치료、예후제공유개치적생물학표지.방법 응용면역조화SP법분별검측50례침윤성유선암여30례유선량성증생병중SOX7、β-cate-nin화cyclin D1단백적표체정황,분석SOX7、β-catenin화cyclin D1단백적표체여침윤성유선암환자적발병년령、종류대소、액와림파결전이、조직학분급、병이학분기(pTNM)、자격소수체(ER)、잉격소수체(PR)、인류표피생장인자수체2(Her-2)화위험도분조지간적관계.결과 재50례침윤성유선암조직중,SOX7화cyclin D1단백적양성표체솔분별위42％(21/50)화70％(35/50),β-catenin단백적이상표체솔위70％(35/50).재침윤성유선암중,SOX7단백적양성솔명현저우유선량성증생병적70％ (21/30) (P =0.021＜0.05),량조간차이유통계학의의；β-catenin단백적이상표체솔화cyclin D1단백적양성표체솔균고우유선량성증생병적43.3％ (13/30) (P =0.033 ＜0.05)화20％ (6/30) (P =0.000＜ 0.001),량조간차이균유통계학의의.재50례침윤성유선암중,SOX7화β-catenin단백동시양성표체12례,동시음성표체5례；SOX7화cyclinD1단백동시양성표체11례,동시음성표체5례；β-catenin화cyclinD1단백동시양성표체28례,동시음성표체8례.경Spearman상관분석,SOX7단백적양성표체분별여p-catenin적이상표체화cyclin D1적양성표체정부상관관계(r=-0.282,P=0.046 ＜0.05;r =-0.327,P =0.020 ＜0.05),β-catcnin적이상표체여cyclin D1적양성표체정정상관관계(r=0.333,P=0.018 ＜0.05).재침윤성유선암중,SOX7단백적표체재발병년령、액와림파결전이、조직학분급、pTNM분기、ER、PR、Her-2화위험도분조간차이유통계학의의(P＜0.05),이재종류직경분조간비교차이무통계학의의(P ＞0.05)；β-catenin단백적이상표체화cyclin D1단백적양성표체재종류직경、액와림파결전이화pTNM분기분조간차이유통계학의의(P＜0.05),이재발병년령、조직학분급、ER、PR、Her-2화위험도분조간비교차이무통계학의의(P＞0.05).결론 SOX7、β-cate-nin화cyclinD1단백재대다수침윤성유선암조직중표체이상,차삼자재침윤성유선암발생、발전과정중가능통과동일통로발휘조절작용.SOX7、β-catenin화cyclin D1단백적표체여침윤성유선암적림상병이학특정지간존재일정적상관성,검측삼자적표체정황가성위림상상판단침윤성유선암적악성정도급예후적중요삼고지표.
Objective This study aimed to investigate the expression of SOX7,β-catenin and cyclin D1 in invasive breast cancer and hyperplastic disease of the breast,and explore their relationship with clinical pathology charactersis in invasive breast cancer in order to provide valuable biomarkers for the treatment and prognosis.Methods The expression of SOX7,β-catenin and cyclin D1 was neasured in 50 invasive breast cancer tissues and 30 hyperplastic disease of the breast by immunohistochemical SP method.The correlations of SOX7,β-catenin and cyclin D1 in invasive breast cancer to clinicopathologic features were analyzed,such as age,size of tumor,axillary lymph node metastasis,histological grade,pTNM stage,ER,PR,Her-2 expression and the risk.Results Immunohistochemical results showed that the positive rates of SOX7 and cyclin D1 in invasive breast cancer were 42％ (21/50)and 70％ (35/50),and the abnormal expression rates of β-catenin in invasive breast cancer was 70％ (35/50).The positive rates of SOX7 in invasive breast cancer was significantly lower than that in hyperplastic disease of the breast 70％ (21/30).The expression of SOX7 had difference between two groups (P =0.021 ＜0.05).The abnormal expression rates of β-catenin and the positive rates of cyclin D1 in invasive breast cancer wcre significantly higher than that in hyperplastic disease of the breast 43.3％ (13/30) (P =0.033 ＜ 0.05) and 20％ (6/30) (P =0.000 ＜ 0.001).The expression of β-catenin and cyclin D1 had difference between two groups.In invasive breast cancer,SOX7 and β-catenin protein expression in 12 cases,while negative expression in 5 cases; SOX7 and cyclin D1 protein positive expression in H 1 cases,while negative expression in 5 cases; β-catenin and cyclin D1 protein positive expression iu 28 cases,while negative expression in 8 cases.Results of the analysis by Spearman showed that in invasive breast cancer the SOX7 protein expression was negatively correlated with the abnormal expression of β-catenin protein and the expression of cyclin D1 protein(r =-0.282,P =0.046 ＜ 0.05 ;r =-0.327,P =0.020 ＜ 0.05)while the abnormal expression of β-catenin protein was positively correlated with the expression of eyclin D1 protein(r =0.333,P =0.018 ＜ 0.05).In invasive breast cancer the expression of SOX7 protein was correlated with age,axillary lymph node metastasis,histological grade,pTNM stage,ER,PR,Her-2 expression and the risk(P ＜0.05),but with no correlation with size of tumor (P ＞ 0.05).However,the abnoral expression of β-catenin and the positive expression of cyclin D1 in invasive breast cancer were correlated with size of tumor,axillary lymph node metastasis and pTNM stage(P ＜ 0.05),but with no correlation with age,histological grade,ER,PR,Her-2 and the risk(P ＞0.05).Conclusions SOX7.β-catenin and cyclin D1 are frequently abnormality-regulated in invasive breast cancer tissues,and the three protein may play a regulatory role through the same pathway in the development and progression of invasive breast cancer.SOX7,β-catenin and cyclin D1,s abnormal expression in invasive breast cancer correlate with the clinical pathology charactersis,and the three protein may be valuable marker for assessing the prognosis for invasive breast cancer.
