国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2013年
10期
655-658
,共4页
张谦%郭东来%韩振魁%斯坎德尔·努尔买买提%肖柯·吐尔迪%李鹏
張謙%郭東來%韓振魁%斯坎德爾·努爾買買提%肖柯·吐爾迪%李鵬
장겸%곽동래%한진괴%사감덕이·노이매매제%초가·토이적%리붕
结肠肿瘤%塞来昔布%增生%COX-2%Survivin
結腸腫瘤%塞來昔佈%增生%COX-2%Survivin
결장종류%새래석포%증생%COX-2%Survivin
Colonic neoplasms%Celecoxib%Hyperplasia%COX-2%Survivin
目的 研究塞来昔布在体外对人结肠癌细胞Caco-2生长增生及其抗肿瘤的相关分子机制.方法 体外培养人结肠癌细胞Caco-2,MTT法检测塞来昔布在相同浓度下,不同时间对于结肠癌细胞增生的影响,并计算IC50值；RT-PCR法检验COX-2、Survivin的mRNA的表达影响.结果 MTT结果显示:相同干预浓度塞来昔布抑制人结肠癌细胞增生,其24 h,48 h,72 h的IC50分别为:(99.519±10.355) μmol/L、(71.546±6.446) μmol/L、(59.622±15.999)μmol/L; RT-PCR结果显示:人结肠癌细胞Caco-2正常对照组及塞来昔布干预组COX-2 mRNA灰度值分别为:0.808 ±0.021、0.101 ±0.002(t=19.037,P=0.000)；Survivin mRNA灰度值分别为:0.798±0.031、0.190 ±0.002(t=18.987,P=0.002).结论 塞来昔布抑制结肠癌细胞增生,塞来昔布抗肿瘤机制可能是通过抑制COX-2及Survivin的mRNA的表达来实现的.
目的 研究塞來昔佈在體外對人結腸癌細胞Caco-2生長增生及其抗腫瘤的相關分子機製.方法 體外培養人結腸癌細胞Caco-2,MTT法檢測塞來昔佈在相同濃度下,不同時間對于結腸癌細胞增生的影響,併計算IC50值；RT-PCR法檢驗COX-2、Survivin的mRNA的錶達影響.結果 MTT結果顯示:相同榦預濃度塞來昔佈抑製人結腸癌細胞增生,其24 h,48 h,72 h的IC50分彆為:(99.519±10.355) μmol/L、(71.546±6.446) μmol/L、(59.622±15.999)μmol/L; RT-PCR結果顯示:人結腸癌細胞Caco-2正常對照組及塞來昔佈榦預組COX-2 mRNA灰度值分彆為:0.808 ±0.021、0.101 ±0.002(t=19.037,P=0.000)；Survivin mRNA灰度值分彆為:0.798±0.031、0.190 ±0.002(t=18.987,P=0.002).結論 塞來昔佈抑製結腸癌細胞增生,塞來昔佈抗腫瘤機製可能是通過抑製COX-2及Survivin的mRNA的錶達來實現的.
목적 연구새래석포재체외대인결장암세포Caco-2생장증생급기항종류적상관분자궤제.방법 체외배양인결장암세포Caco-2,MTT법검측새래석포재상동농도하,불동시간대우결장암세포증생적영향,병계산IC50치；RT-PCR법검험COX-2、Survivin적mRNA적표체영향.결과 MTT결과현시:상동간예농도새래석포억제인결장암세포증생,기24 h,48 h,72 h적IC50분별위:(99.519±10.355) μmol/L、(71.546±6.446) μmol/L、(59.622±15.999)μmol/L; RT-PCR결과현시:인결장암세포Caco-2정상대조조급새래석포간예조COX-2 mRNA회도치분별위:0.808 ±0.021、0.101 ±0.002(t=19.037,P=0.000)；Survivin mRNA회도치분별위:0.798±0.031、0.190 ±0.002(t=18.987,P=0.002).결론 새래석포억제결장암세포증생,새래석포항종류궤제가능시통과억제COX-2급Survivin적mRNA적표체래실현적.
Objective To study celecoxib in vitro human colon cancer cell growth and proliferation of Caco-2and its anti-tumor molecular mechanisms.Methods Cultured human colon cancer Caco-2,MTT method analyses celecoxib at the same concentration,at different times for the colon cancer cell proliferation and calculate IC50 value.RT-PCR method used to evaluate the expression of COX-2,Survivin mRNA.Results MTT assay showed:the concentration of the same intervention celecoxib to inhibit the proliferation of human colon cancer cells,its 24 h,48 h,72 h IC50 of respectively:(99.519 ± 10.355) μmol/L,(71.546 ± 6.446) μmol/L,(59.622 ± 15.999) μmol/L.The RT-PCR results show:human colon canoer cells Caco-2 normal control group and celecoxib in the intervention group,respectively,the COX-2 mRNA gray value for:0.873 ±0.026,0.115 ±0.008,P ＜ 0.05 ; SURVIVIN mRNA gray values were:0.808 ± 0.021,0.101 ± 0.002 (t =19.037,P =0.000) ; Survivin mRNA were0.798±0.031,0.190±0.002(t=18.987,P=0.002).Conclusions Celecoxib inhibition of colon cancer cell proliferation,celecoxib antitumor mechanisms may by inhibiting the expression of COX-2 and Survivin mRNA in implementation.
