CAJ | 학술논문

目的通过对胃癌新鲜离体组织进行傅里叶变换红外光谱测定,探讨其区分胃癌组织和正常胃组织的可行性及机制.方法联合应用衰减全反射探头及中红外光纤、傅里叶变换红外光谱仪,检测胃癌手术中切除的新鲜离体胃癌组织50例.比较胃癌组织和正常胃组织红外光谱各谱带的相对峰位、相对峰强.结果与蛋白、脂质、糖类和核酸相关的谱带在胃癌组织和正常胃组织之间差异明显具有统计学意义(P＜0.05).结论傅里叶红外光谱技术可区别新鲜离体状态下胃癌组织和正常胃组织,联合应用衰减全反射探头及中红外光纤、傅里叶变换红外光谱仪有望成为胃癌诊断及术中导航的新方法.
목적 통과대위암신선리체조직진행부리협변환홍외광보측정,탐토기구분위암조직화정상위조직적가행성급궤제.방법 연합응용쇠감전반사탐두급중홍외광섬、부리협변환홍외광보의,검측위암수술중절제적신선리체위암조직50례.비교위암조직화정상위조직홍외광보각보대적상대봉위、상대봉강.결과 여단백、지질、당류화핵산상관적보대재위암조직화정상위조직지간차이명현구유통계학의의(P＜0.05).결론 부리협홍외광보기술가구별신선리체상태하위암조직화정상위조직,연합응용쇠감전반사탐두급중홍외광섬、부리협변환홍외광보의유망성위위암진단급술중도항적신방법.
Objective To identify the FTIR spectroscopic characterization of breast cancer and explore the possibility of application of FTIR in differentiation of malignant and benign gastric lesions.Methods FTIR spectra of surgically removed fresh gastic tissues were measured by spectrometer equipped with mid-infrared fiber optics and an ATR probe.Peaks in the spectra were measured and relative intensity ratios were calculated and analyzed if there are significant differences between the spectra of malignant and benign gastic lesions.Results There were significant differences between the spectra of malignant gastic cancers and benign gastic tissues in protein structures 、lipid structures、carbohydrate and nucleic acid.Conclusions FTIR spectroscopy could be a useful tool in clinical diagnosis of gastic cancer and new methods of intraoperative navigation.