CAJ | 학술논문

报告基因是现代分子生物学研究领域中用于分析结构基因旁侧区域潜在的顺式元件(如启动子、增强子和沉默子等)和反式作用因子相互作用关系的一种重要工具,通过它的表达产物便捷、可靠、灵敏地解析基因时空表达调控的内在规律.报告基因技术广泛应用于基因表达调控、信号转导、转基因、启动子分析、受体功能鉴定、基因治疗以及药物筛选等领域,该文将对报告基因的主要类型、特点及其应用研究进展进行综述.
보고기인시현대분자생물학연구영역중용우분석결구기인방측구역잠재적순식원건(여계동자、증강자화침묵자등)화반식작용인자상호작용관계적일충중요공구,통과타적표체산물편첩、가고、령민지해석기인시공표체조공적내재규률.보고기인기술엄범응용우기인표체조공、신호전도、전기인、계동자분석、수체공능감정、기인치료이급약물사선등영역,해문장대보고기인적주요류형、특점급기응용연구진전진행종술.
Reporter gene is an important tool of analyzing the relationship of the potential cis-acting elements (promoter,enhancer and silencer,etc.) and trans-acting factors in the research field of modem molecular biology.It is convenient、sensitive and suitable in analyzing molecular mechanism of gene temporal and spatial regulation and expression through their expression products.Reporter gene technology has been extensively applied in many fields,including regulation of gene expression,signal transduction,genetic modification,analysis of promoter,identification of acceptors function,gene therapy,and drug screening,etc.The present paper reviewed the main types,characteristics,and applications of the reporter genes.