CAJ | 학술논문

扫描电子显微镜(scanning electron microscopy,SEM)是高分辨率观察染色体及其超微结构的必要工具之一.应用SEM可以进行染色体构造和高级染色质结构的三维高精度成像和研究.随着人工解凝缩方法和染色体标记技术的发展,实现了染色体结构单元上DNA和蛋白质的分布检测,以及功能单元(如DNA序列、特殊蛋白、表观修饰)的定位.最新发展的SEM在离子液体中观察染色体技术必然会为染色体研究提供一个新视角.
소묘전자현미경(scanning electron microscopy,SEM)시고분변솔관찰염색체급기초미결구적필요공구지일.응용SEM가이진행염색체구조화고급염색질결구적삼유고정도성상화연구.수착인공해응축방법화염색체표기기술적발전,실현료염색체결구단원상DNA화단백질적분포검측,이급공능단원(여DNA서렬、특수단백、표관수식)적정위.최신발전적SEM재리자액체중관찰염색체기술필연회위염색체연구제공일개신시각.
Scanning electron microscopy (SEM) is one of the indispensable tools for high-resolution visualization of chromosomes and their ultrastructure.SEM can be applied to high-accuracy three-dimensional imaging for studying chromosome architecture and higher-order chromatin structure.With improvement in artificial decondensation and chromosome labeling techniques,structural elements of chromosome,including DNA and protein distribution,can be detected.Functional elements (e.g.DNA sequences,specific proteins,epigenetic modifications) also can be located.Techniques in electron microscopy using ionic liquid will have to open a new view of studying chromosomes with microscopy.