CAJ | 학술논문

原子力显微镜(atomic force microscope,AFM)能够在生理状态下获得样本表面结构的纳米级三维图像以及生物材料的力学性能,近年来被广泛用于生命科学研究领域,从单细胞到组织器官均取得了一定成果.眼球作为人体重要的器官组织,其结构和力学性能的变化与疾病的发生密切相关,AFM在对眼部各组织的超微结构观察以及生物力学测量为研究疾病发生机制做出了较大贡献.
원자력현미경(atomic force microscope,AFM)능구재생리상태하획득양본표면결구적납미급삼유도상이급생물재료적역학성능,근년래피엄범용우생명과학연구영역,종단세포도조직기관균취득료일정성과.안구작위인체중요적기관조직,기결구화역학성능적변화여질병적발생밀절상관,AFM재대안부각조직적초미결구관찰이급생물역학측량위연구질병발생궤제주출료교대공헌.
This review briefly introduces the principles of the atomic force microscopy (AFM) and its applications in ophthalmology.The AFM is an instrument that traces the surface topography of a sample with a sharp probe,while monitoring the interaction forces working between the probe and the sample surface.With AFM,the three-dimensional image of the surface can be obtained in nearly physiological conditions at a nanoscale resolution,as well as the biomechanics of the sample.The domains of AFM application have increased dramatically in recent years,from single molecules to organs.In ophthalmology,AFM demonstrates great advantages in the studies on eye microstructures and biomechanical properties,such as the cornea,sclera and lens.Although there are some innate limitations for AFM imaging and biomechanics measurement,AFM has great potential for providing valuable new informations in ophthalmology.