国际移植与血液净化杂志
國際移植與血液淨化雜誌
국제이식여혈액정화잡지
INTERNATIONAL JOURNAL OF TRANSPLANTATION AND HEMOPURIFICATION
2014年
4期
34-37
,共4页
张亚玲%李荣山%乔玉峰%罗阳燕
張亞玲%李榮山%喬玉峰%囉暘燕
장아령%리영산%교옥봉%라양연
三氟拉嗪%系膜细胞%血小板源生长因子%凋亡
三氟拉嗪%繫膜細胞%血小闆源生長因子%凋亡
삼불랍진%계막세포%혈소판원생장인자%조망
Trifluoperazine%Human mesangial cells%Platelet-derived growth factor%Apoptosis
目的 探讨三氟拉嗪对血小板源生长因子刺激增殖的人肾小球系膜细胞凋亡的影响.方法 取对数生长期的人肾小球系膜细胞,血小板源生长因子刺激24h后采用0、10、20、30 μmol/L三氟拉嗪处理48 h;20 μmol/L三氟拉嗪处理0h、24h、48 h、72 h,运用流式细胞仪检测上述各组系膜细胞凋亡情况.20 μmol/L三氟拉嗪作用48h后,采用Western blot检测Caspase-3、Bcl-2和Bax蛋白表达水平.结果 (1)与空白对照组相比,血小板源生长因子组人肾小球系膜细胞凋亡明显减少,而10、20、30 μmol/L三氟拉嗪组人肾小球系膜细胞凋亡较血小板源生长因子组明显增加(P＜0.05),其中以20 μmol/L三氟拉嗪组增加更为明显(P＜0.01).(2)三氟拉嗪处理后,人肾小球系膜细胞凋亡明显增加,且随着时间延长,凋亡率逐渐增加(P＜0.05).(3)与空白对照组比较,血小板源生长因子组Caspase-3活性片段及Bax表达明显降低,而三氟拉嗪组较血小板源生长因子组明显增高;血小板源生长因子组Bcl-2表达明显增高,而三氟拉嗪组较血小板源生长因子组明显降低(P＜0.05).结论 三氟拉嗪能够浓度和时间依赖性地诱导人肾小球系膜细胞凋亡,其机制可能与改变Bcl-2/Bax比值、激活Caspase-3有关.
目的 探討三氟拉嗪對血小闆源生長因子刺激增殖的人腎小毬繫膜細胞凋亡的影響.方法 取對數生長期的人腎小毬繫膜細胞,血小闆源生長因子刺激24h後採用0、10、20、30 μmol/L三氟拉嗪處理48 h;20 μmol/L三氟拉嗪處理0h、24h、48 h、72 h,運用流式細胞儀檢測上述各組繫膜細胞凋亡情況.20 μmol/L三氟拉嗪作用48h後,採用Western blot檢測Caspase-3、Bcl-2和Bax蛋白錶達水平.結果 (1)與空白對照組相比,血小闆源生長因子組人腎小毬繫膜細胞凋亡明顯減少,而10、20、30 μmol/L三氟拉嗪組人腎小毬繫膜細胞凋亡較血小闆源生長因子組明顯增加(P＜0.05),其中以20 μmol/L三氟拉嗪組增加更為明顯(P＜0.01).(2)三氟拉嗪處理後,人腎小毬繫膜細胞凋亡明顯增加,且隨著時間延長,凋亡率逐漸增加(P＜0.05).(3)與空白對照組比較,血小闆源生長因子組Caspase-3活性片段及Bax錶達明顯降低,而三氟拉嗪組較血小闆源生長因子組明顯增高;血小闆源生長因子組Bcl-2錶達明顯增高,而三氟拉嗪組較血小闆源生長因子組明顯降低(P＜0.05).結論 三氟拉嗪能夠濃度和時間依賴性地誘導人腎小毬繫膜細胞凋亡,其機製可能與改變Bcl-2/Bax比值、激活Caspase-3有關.
목적 탐토삼불랍진대혈소판원생장인자자격증식적인신소구계막세포조망적영향.방법 취대수생장기적인신소구계막세포,혈소판원생장인자자격24h후채용0、10、20、30 μmol/L삼불랍진처리48 h;20 μmol/L삼불랍진처리0h、24h、48 h、72 h,운용류식세포의검측상술각조계막세포조망정황.20 μmol/L삼불랍진작용48h후,채용Western blot검측Caspase-3、Bcl-2화Bax단백표체수평.결과 (1)여공백대조조상비,혈소판원생장인자조인신소구계막세포조망명현감소,이10、20、30 μmol/L삼불랍진조인신소구계막세포조망교혈소판원생장인자조명현증가(P＜0.05),기중이20 μmol/L삼불랍진조증가경위명현(P＜0.01).(2)삼불랍진처리후,인신소구계막세포조망명현증가,차수착시간연장,조망솔축점증가(P＜0.05).(3)여공백대조조비교,혈소판원생장인자조Caspase-3활성편단급Bax표체명현강저,이삼불랍진조교혈소판원생장인자조명현증고;혈소판원생장인자조Bcl-2표체명현증고,이삼불랍진조교혈소판원생장인자조명현강저(P＜0.05).결론 삼불랍진능구농도화시간의뢰성지유도인신소구계막세포조망,기궤제가능여개변Bcl-2/Bax비치、격활Caspase-3유관.
Objective To explore the effect of trifluoperazine (TFP) on platelet-derived growth factor (PDGF)-induced cultured human mesangial cell (HMC) apoptosis.Methods The HMCs of logarithmic phase were stimulated with PDGF for 24 h and cultured with 0,10,20 or 30 μmol/L TFP for 48 h.20 μmol/L TFP was used for 0,24,48 or 72 h before flow cytometry was performed to detect the apoptosis of the mesangial cells.The expression of Caspase-3,Bax and Bcl-2 was analyzed after incubated with 20μmol/L of TFP for 48 h by Western blot.Results (1) Compared with the control group,the apoptosis of the PDGF group was significantly decreased (P ＜ 0.05).While compared with the PDGF group,10,20 and 30 μmol/L TFP group's figure was significantly increased (P ＜ 0.05),of which 20 μmol/L TFP group was increased more obviously (P ＜ 0.01).(2)The apoptosis of HMCs was significantly increased in a time-dependent manner after incubated with TFP for 72 h.(3) Compared with the control group,the expression of Caspase 3 and Bax was significantly decreased in the PDGF group,while the expression of Bcl-2 was significantly increased.Conclusions TFP could induce apoptosis in a concentrationand time-dependent manner.The mechanism may be related to the change of Bcl-2/Bax and the activation of Caspase-3.
