国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2013年
1期
75-80
,共6页
乳腺肿瘤%受体,CCR4%趋化因子CXCL12
乳腺腫瘤%受體,CCR4%趨化因子CXCL12
유선종류%수체,CCR4%추화인자CXCL12
Breast neoplasms%Receptors,CCR4%Chemokine CXCL12
目的 分析乳腺癌中趋化因子受体4(CXCR4)、趋化因子12(CXCL12)与人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(PTEN)的表达及临床意义.方法 采用免疫组织化学技术SABC法检测60例乳腺癌组织标本CXCR4、CXCL12、PTEN的表达情况,分析乳腺癌中CXCR4、CXCL12及PTEN表达与年龄、肿瘤大小、组织学分级、TNM分期、淋巴结转移、雌激素受体(ER)、人类表皮生长因子2 (Her-2)及脉管侵犯的关系.另取20例乳腺纤维腺瘤及20例癌旁正常乳腺组织作为对照组.结果 免疫组织化学结果显示,CXCR4(x2=48.754,P=0.000)、CXCL12(x2=47.611,P=0.000)及PTEN(x2=19.994,P=0.000)在乳腺癌、乳腺纤维腺瘤与癌旁正常组织中的表达差异均具有统计学意义,其中CXCR4、CXCL12阳性表达与乳腺癌的组织学分级(x2=11.080,P=0.004;x2 =6.978,P=0.031)、TNM分期(x2=9.819,P=0.007;x2 =10.163,P=0.006)、淋巴结转移(x2=6.213,P=0.013;x2=8.031,P=0.005)、ER(x2=12.774,P=0.000;x2 =7.330,P=0.007)、脉管侵犯(x2 =5.860,P=0.013;x2=5.185,P=0.020)及Her-2(x2=5.487,P=0.019；x2=4.689,P=0.030)相关；PTEN在乳腺癌组织中的表达与TNM分期(x2=7.366,P=0.025)、淋巴结转移(x2=5.511,P=0.019)及ER(x2=4.077,P =0.043)状态相关.CXCR4与CXCL12在乳腺癌中的表达呈正相关(r=0.336,P=0.004)；CXCR4与PTEN、CXCL12与PTEN在乳腺癌中的表达呈负相关(r=-0.362,P=0.004；r=-0.360,P=0.004).结论 趋化因子CXCL12及其受体CXCR4的高表达及PTEN的低表达与乳腺癌的发生和转移有关,可能在促进乳腺癌的生长和发展中起重要作用.
目的 分析乳腺癌中趨化因子受體4(CXCR4)、趨化因子12(CXCL12)與人第10號染色體缺失的燐痠酶及張力蛋白同源的基因(PTEN)的錶達及臨床意義.方法 採用免疫組織化學技術SABC法檢測60例乳腺癌組織標本CXCR4、CXCL12、PTEN的錶達情況,分析乳腺癌中CXCR4、CXCL12及PTEN錶達與年齡、腫瘤大小、組織學分級、TNM分期、淋巴結轉移、雌激素受體(ER)、人類錶皮生長因子2 (Her-2)及脈管侵犯的關繫.另取20例乳腺纖維腺瘤及20例癌徬正常乳腺組織作為對照組.結果 免疫組織化學結果顯示,CXCR4(x2=48.754,P=0.000)、CXCL12(x2=47.611,P=0.000)及PTEN(x2=19.994,P=0.000)在乳腺癌、乳腺纖維腺瘤與癌徬正常組織中的錶達差異均具有統計學意義,其中CXCR4、CXCL12暘性錶達與乳腺癌的組織學分級(x2=11.080,P=0.004;x2 =6.978,P=0.031)、TNM分期(x2=9.819,P=0.007;x2 =10.163,P=0.006)、淋巴結轉移(x2=6.213,P=0.013;x2=8.031,P=0.005)、ER(x2=12.774,P=0.000;x2 =7.330,P=0.007)、脈管侵犯(x2 =5.860,P=0.013;x2=5.185,P=0.020)及Her-2(x2=5.487,P=0.019；x2=4.689,P=0.030)相關；PTEN在乳腺癌組織中的錶達與TNM分期(x2=7.366,P=0.025)、淋巴結轉移(x2=5.511,P=0.019)及ER(x2=4.077,P =0.043)狀態相關.CXCR4與CXCL12在乳腺癌中的錶達呈正相關(r=0.336,P=0.004)；CXCR4與PTEN、CXCL12與PTEN在乳腺癌中的錶達呈負相關(r=-0.362,P=0.004；r=-0.360,P=0.004).結論 趨化因子CXCL12及其受體CXCR4的高錶達及PTEN的低錶達與乳腺癌的髮生和轉移有關,可能在促進乳腺癌的生長和髮展中起重要作用.
목적 분석유선암중추화인자수체4(CXCR4)、추화인자12(CXCL12)여인제10호염색체결실적린산매급장력단백동원적기인(PTEN)적표체급림상의의.방법 채용면역조직화학기술SABC법검측60례유선암조직표본CXCR4、CXCL12、PTEN적표체정황,분석유선암중CXCR4、CXCL12급PTEN표체여년령、종류대소、조직학분급、TNM분기、림파결전이、자격소수체(ER)、인류표피생장인자2 (Her-2)급맥관침범적관계.령취20례유선섬유선류급20례암방정상유선조직작위대조조.결과 면역조직화학결과현시,CXCR4(x2=48.754,P=0.000)、CXCL12(x2=47.611,P=0.000)급PTEN(x2=19.994,P=0.000)재유선암、유선섬유선류여암방정상조직중적표체차이균구유통계학의의,기중CXCR4、CXCL12양성표체여유선암적조직학분급(x2=11.080,P=0.004;x2 =6.978,P=0.031)、TNM분기(x2=9.819,P=0.007;x2 =10.163,P=0.006)、림파결전이(x2=6.213,P=0.013;x2=8.031,P=0.005)、ER(x2=12.774,P=0.000;x2 =7.330,P=0.007)、맥관침범(x2 =5.860,P=0.013;x2=5.185,P=0.020)급Her-2(x2=5.487,P=0.019；x2=4.689,P=0.030)상관；PTEN재유선암조직중적표체여TNM분기(x2=7.366,P=0.025)、림파결전이(x2=5.511,P=0.019)급ER(x2=4.077,P =0.043)상태상관.CXCR4여CXCL12재유선암중적표체정정상관(r=0.336,P=0.004)；CXCR4여PTEN、CXCL12여PTEN재유선암중적표체정부상관(r=-0.362,P=0.004；r=-0.360,P=0.004).결론 추화인자CXCL12급기수체CXCR4적고표체급PTEN적저표체여유선암적발생화전이유관,가능재촉진유선암적생장화발전중기중요작용.
Objective To analyse the expressions and clinical significances of CXCR4,CXCL12 and PTEN in breast cancer.Methods The expressions of CXCR4,CXCL12 and PTEN in 60 cases of breast cancer were detected by immunohistochemistry technique SABC method.The correlations of levels of CXCR4,CXCL12 and PTEN expression and the age of patient,tumor diameter,histological grade,TNM stage,lymph node metastasis,ER status,Her-2 status,and vessel invasion were analysed.Twenty cases of breast fibroadenoma tissues and 20 cases of normal breast tissues were analysed as controls.Results The expressions of CXCR4 (x2 =48.754,P =0.000),CXCL12 (x2 =47.611,P =0.000) and PTEN (x2 =19.994,P =0.000) in breast cancer,normal breast tissues and breast fobroadenoma showed significant differences.The positive expressions of CXCR and CXCL12 were significantly correlated with histological grade (x2 =11.080,P =0.004;x2 =6.978,P =0.031),TNM stage (x2 =9.819,P =0.007;X2 =10.163,P =0.006),lymph node metastasis (x2 =6.213,P =0.013;x2 =8.031,P =0.005),ER (x2 =12.774,P =0.000;x2 =7.330,P=0.007),vessel invasion (x2 =5.860,P=0.013; x2 =5.185,P=0.020) and Her-2 (x2 =5.487,P =0.019;x2 =4.689,P =0.030).The expression of PTEN in breast cancer was significantly correlated with TNM stage (x2=7.366,P =0.025),lymph node metastasis (x2 =5.511,P =0.019) and ER state (x2 =4.077,P =0.043).There was a positive correlation between the expression of CXCR4 and the expression of CXCL12 in breast cancer (r =0.336,P =0.004).There was a negative correlation between the expression of CXCR4 and the expression of PTEN in breast cancer (r =-0.362,P =0.004).There was a negative correlation between the expression of CXCL12 and the expression of PTEN in breast cancer (r =-0.360,P =0.004).Conclusion The high expression of the chemokine CXCL12 and its receptor CXCR4 and the low expression of PTEN are closely related to the carcinogenesis and metastasis of breast cancer,which may play an important role in the development of breast cancer.
