国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2013年
3期
232-235
,共4页
徐杰%许晓群%邹志孟%苏庆红%张建华%王郡甫
徐傑%許曉群%鄒誌孟%囌慶紅%張建華%王郡甫
서걸%허효군%추지맹%소경홍%장건화%왕군보
乳腺肿瘤%细胞系,肿瘤%受体,雌激素%白藜芦醇
乳腺腫瘤%細胞繫,腫瘤%受體,雌激素%白藜蘆醇
유선종류%세포계,종류%수체,자격소%백려호순
Breast neoplasms%Cell line,tumor%Receptors,estrogen%Resveratrol
目的 探讨白藜芦醇(Res)对雌激素受体(HE)表达不同的3种乳腺癌细胞DU4475、MDA-MB-231和MCF-7增殖、周期和凋亡的影响.方法 药物处理组加入终浓度为6.25、12.5、25、50、100、200 μmol/L的Res,分别设24、48、72h3个时期.采用噻唑蓝比色法(MTT)法检测Res对乳腺癌细胞增殖的影响,流式细胞术(FCM)检测细胞周期及凋亡,药物浓度为0、12.5、25、50 μmol/L.应用统计软件SPSS17.0进行统计学处理.结果 经Res作用后,3种乳腺癌细胞增殖均受到不同程度抑制.其中Res作用48 h和72 h,各浓度对细胞的抑制均有统计学意义(F=15.26,P＜0.05),对ER阴性DU4475和MDA-MB-231细胞株的抑制率均高于ER阳性的MCF-7细胞株.FCM检测结果显示,Res与乳腺癌细胞作用48 h,Res浓度在12.5 ～50 μmol/L时,3种乳腺癌细胞周期均被阻滞于S期,阻滞比例差异有统计学意义(F=34.81,P＜0.05),DU4475和MDA-MB-231阻滞于S期的比例高于MCF-7.100μmol/L时,Res对DU4475的促凋亡作用明显强于MCF-7细胞(t=16.082,P＜0.05).结论 Res可抑制以上3种乳腺癌细胞增殖,导致细胞周期改变,促进凋亡.Res对DU4475和MDA-MB-231细胞增殖抑制作用强于ER阳性MCF-7细胞.
目的 探討白藜蘆醇(Res)對雌激素受體(HE)錶達不同的3種乳腺癌細胞DU4475、MDA-MB-231和MCF-7增殖、週期和凋亡的影響.方法 藥物處理組加入終濃度為6.25、12.5、25、50、100、200 μmol/L的Res,分彆設24、48、72h3箇時期.採用噻唑藍比色法(MTT)法檢測Res對乳腺癌細胞增殖的影響,流式細胞術(FCM)檢測細胞週期及凋亡,藥物濃度為0、12.5、25、50 μmol/L.應用統計軟件SPSS17.0進行統計學處理.結果 經Res作用後,3種乳腺癌細胞增殖均受到不同程度抑製.其中Res作用48 h和72 h,各濃度對細胞的抑製均有統計學意義(F=15.26,P＜0.05),對ER陰性DU4475和MDA-MB-231細胞株的抑製率均高于ER暘性的MCF-7細胞株.FCM檢測結果顯示,Res與乳腺癌細胞作用48 h,Res濃度在12.5 ～50 μmol/L時,3種乳腺癌細胞週期均被阻滯于S期,阻滯比例差異有統計學意義(F=34.81,P＜0.05),DU4475和MDA-MB-231阻滯于S期的比例高于MCF-7.100μmol/L時,Res對DU4475的促凋亡作用明顯彊于MCF-7細胞(t=16.082,P＜0.05).結論 Res可抑製以上3種乳腺癌細胞增殖,導緻細胞週期改變,促進凋亡.Res對DU4475和MDA-MB-231細胞增殖抑製作用彊于ER暘性MCF-7細胞.
목적 탐토백려호순(Res)대자격소수체(HE)표체불동적3충유선암세포DU4475、MDA-MB-231화MCF-7증식、주기화조망적영향.방법 약물처리조가입종농도위6.25、12.5、25、50、100、200 μmol/L적Res,분별설24、48、72h3개시기.채용새서람비색법(MTT)법검측Res대유선암세포증식적영향,류식세포술(FCM)검측세포주기급조망,약물농도위0、12.5、25、50 μmol/L.응용통계연건SPSS17.0진행통계학처리.결과 경Res작용후,3충유선암세포증식균수도불동정도억제.기중Res작용48 h화72 h,각농도대세포적억제균유통계학의의(F=15.26,P＜0.05),대ER음성DU4475화MDA-MB-231세포주적억제솔균고우ER양성적MCF-7세포주.FCM검측결과현시,Res여유선암세포작용48 h,Res농도재12.5 ～50 μmol/L시,3충유선암세포주기균피조체우S기,조체비례차이유통계학의의(F=34.81,P＜0.05),DU4475화MDA-MB-231조체우S기적비례고우MCF-7.100μmol/L시,Res대DU4475적촉조망작용명현강우MCF-7세포(t=16.082,P＜0.05).결론 Res가억제이상3충유선암세포증식,도치세포주기개변,촉진조망.Res대DU4475화MDA-MB-231세포증식억제작용강우ER양성MCF-7세포.
Objective To investigate the effects of resveratrol (Res) on the proliferation,cell cycle and apoptosis of three breast cell lines DU4475,MDA-MB-23land MCF-7 with different estrogen receptor (ER) expressions.Methods Res was added to the drug treatment group at 6.25,12.5,25,50,100 and 200 μmol/L,respectively.Three observation periods at 24,48 and 72 hours were set up respectively.MTT assay was used to detect the effects of Res on proliferation of breast cells.Cell cycle and apoptosis were analyzed by flow cytometry (FCM).The concentrations of drugs were 0,12.5,25,50 μmol/L.The results were analysed by the statistical software SPSS17.0.Results After Res intervention,the proliferations of three cell lines were inhibited to different extent.After 48 and 72 hours of Res,inhibitions of Res with different concentration were significant different (F =15.26,P ＜ 0.05).Inhibition rates of DU4475 and MDA-MB-231 with ER-negative were higer than that of MCF-7 with ER-positive.FCM results prompted that these three kinds of cells were blocked in S phase after 48 hours treatment of 12.5-50 μmol/L Res and the block percentages had significant difference (F =34.81,P ＜ 0.05).The percentages of S phase for DU4475 and MDA-MB-231 arresting were higher than that of MCF-7.For DU4475,the apoptotic and necrosis percentage were higher than that of MCF-7 at 100 μmol/L (t =16.082,P ＜0.05).Conclusion Res can inhibit proliferation,induce cell cycle changing and apoptosis of DU4475,MDA-MB-231 and MCF-7 cells.The inhibitions of Res on DU4475 and MDA-MB-231 are better than that of MCF-7 with ER-positive.
