国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2013年
9期
715-720
,共6页
结肠肿瘤%受体,EphA8%血管生成素类
結腸腫瘤%受體,EphA8%血管生成素類
결장종류%수체,EphA8%혈관생성소류
Colonic neoplasms%Receptor,EphA8%Angiopoietins
目的 探讨EphA8在结肠癌组织中的表达情况和临床意义.方法 采用实时定量RT-PCR和Western blot检测EphA8 mRNA和蛋白在1种正常结肠黏膜细胞株和2种结肠癌细胞株中的表达情况.采用免疫组织化学法检测98例结肠癌组织和12例正常结肠组织中EphA8和血管内皮生长因子(VEGF)的蛋白表达情况,同时使用CD31染色检测肿瘤微血管密度(MVD)情况,并分析EphA8与VEGF和MVD的关系及其与病理分期、预后的关系.结果 EphA8的mRNA在结肠癌细胞株的表达明显高于正常结肠黏膜细胞株(t=11.98,13.54,均P＜0.001).EphA8蛋白在结肠癌细胞株的表达也明显高于正常结肠黏膜细胞株(=4.63,P=0.006;t =4.92,P=0.004).免疫组织化学结果表明EphA8蛋白在结肠癌组织高表达,且癌组织中EphA8的蛋白表达与肿瘤大小(X2=22.97,P＜0.001)、分期(P＜0.001)、分化程度(P=0.007)、淋巴结转移(P ＜0.001)、远处转移(x2=6.97,P=0.008)以及患者生存率(x2=17.3,P＜0.001)存在显著相关性,而与患者的性别和年龄没有明显相关性(X2=1.36,P=0.30；0=0.83,P=0.44).此外,EphA8与VEGF和MVD的表达呈正相关(r=0.434,P＜0.001；r=0.584,P＜0.001).结论 EphA8在结肠癌的恶性发展和转移中有重要作用,因此,EphA8对于结肠癌的治疗干预和预后判断具有潜在的临床应用价值.
目的 探討EphA8在結腸癌組織中的錶達情況和臨床意義.方法 採用實時定量RT-PCR和Western blot檢測EphA8 mRNA和蛋白在1種正常結腸黏膜細胞株和2種結腸癌細胞株中的錶達情況.採用免疫組織化學法檢測98例結腸癌組織和12例正常結腸組織中EphA8和血管內皮生長因子(VEGF)的蛋白錶達情況,同時使用CD31染色檢測腫瘤微血管密度(MVD)情況,併分析EphA8與VEGF和MVD的關繫及其與病理分期、預後的關繫.結果 EphA8的mRNA在結腸癌細胞株的錶達明顯高于正常結腸黏膜細胞株(t=11.98,13.54,均P＜0.001).EphA8蛋白在結腸癌細胞株的錶達也明顯高于正常結腸黏膜細胞株(=4.63,P=0.006;t =4.92,P=0.004).免疫組織化學結果錶明EphA8蛋白在結腸癌組織高錶達,且癌組織中EphA8的蛋白錶達與腫瘤大小(X2=22.97,P＜0.001)、分期(P＜0.001)、分化程度(P=0.007)、淋巴結轉移(P ＜0.001)、遠處轉移(x2=6.97,P=0.008)以及患者生存率(x2=17.3,P＜0.001)存在顯著相關性,而與患者的性彆和年齡沒有明顯相關性(X2=1.36,P=0.30；0=0.83,P=0.44).此外,EphA8與VEGF和MVD的錶達呈正相關(r=0.434,P＜0.001；r=0.584,P＜0.001).結論 EphA8在結腸癌的噁性髮展和轉移中有重要作用,因此,EphA8對于結腸癌的治療榦預和預後判斷具有潛在的臨床應用價值.
목적 탐토EphA8재결장암조직중적표체정황화림상의의.방법 채용실시정량RT-PCR화Western blot검측EphA8 mRNA화단백재1충정상결장점막세포주화2충결장암세포주중적표체정황.채용면역조직화학법검측98례결장암조직화12례정상결장조직중EphA8화혈관내피생장인자(VEGF)적단백표체정황,동시사용CD31염색검측종류미혈관밀도(MVD)정황,병분석EphA8여VEGF화MVD적관계급기여병리분기、예후적관계.결과 EphA8적mRNA재결장암세포주적표체명현고우정상결장점막세포주(t=11.98,13.54,균P＜0.001).EphA8단백재결장암세포주적표체야명현고우정상결장점막세포주(=4.63,P=0.006;t =4.92,P=0.004).면역조직화학결과표명EphA8단백재결장암조직고표체,차암조직중EphA8적단백표체여종류대소(X2=22.97,P＜0.001)、분기(P＜0.001)、분화정도(P=0.007)、림파결전이(P ＜0.001)、원처전이(x2=6.97,P=0.008)이급환자생존솔(x2=17.3,P＜0.001)존재현저상관성,이여환자적성별화년령몰유명현상관성(X2=1.36,P=0.30；0=0.83,P=0.44).차외,EphA8여VEGF화MVD적표체정정상관(r=0.434,P＜0.001；r=0.584,P＜0.001).결론 EphA8재결장암적악성발전화전이중유중요작용,인차,EphA8대우결장암적치료간예화예후판단구유잠재적림상응용개치.
Objective To investigate the expression of EphA8 in colon cancer and its clinical significance.Methods Real-time quantitative polymerase chain reaction (RT-PCR) and Western blot were respectively used to assess the expressions of EphA8 mRNA and protein in normal colon mucosa cell line and colon cancer cell lines.Immunohistochemistry for EphA8 and vascular endothelial growth factor (VEGF) were performed in 98 cases of colon cancer and 12 matched normal mucosa tissues.CD31 immunohistochemical staining was used for microvascular density (MVD) counting.The relationships between the expression of EphA8 and the expression of VEGF and MVD,the clinical pathological significance,and the prognosis of patients were analyzed by statistical methods.Results EphA8 mRNA expressions were significantly higher in colon cancer cell lines than those in normal mucosa cell line (t =11.98,13.54,P ＜0.001).EphA8 protein expressions were significantly higher in colon cancer cell lines than those in normal mucosa cell line (t =4.63,P =0.006;t =4.92,P =0.004).The high expression of EphA8 was closely related to tumor size (x2 =22.97,P＜0.001),TNM stage (P＜0.001),differentiated degree (P =0.007)and lymph node metastasis (P＜0.001),distant metastasis (x2 =6.97,P =0.008) and poor survival rote (x2 =17.3,P ＜0.001).But it was not associated with the gender and age (x2 =1.36,P =0.30; x2 =0.83,P=0.44).Besides,EphA8 had positive correlation with the VEGF and MVD (r =0.434,P ＜0.001; r =0.584,P ＜0.001).Conclusion EphA8 may play important roles in the development and metastasis of colon cancer,which has potential clinical values in the therapeutic intervention and prognosis of colon cancer.
