CAJ | 학술논문

目的通过研究磺基转移酶CHST13在人肝癌高转移细胞株MHCC97-H和人肝癌低转移细胞株MHCC97-L中的差异表达,明确其与肝癌转移的相关性,从而证实肝癌转移诊断及抗肿瘤治疗新靶点.方法采用实时PCR、Western blot分析磺基转移酶CHST13在人肝癌高、低转移细胞株的差异表达,通过RNA干扰技术干预CHST13基因,检测干扰前后MHCC97-L细胞的体外侵袭力及体内成瘤性.结果 CHST13在人肝癌高、低转移细胞株中表达具有明显差异;下调MHCC97-L细胞中CHST13基因表达后,穿过基底膜的细胞数量[(30±3)个]明显多于未处理组[(14±2)个],体外侵袭力显著增强(t=2.8,P=0.005);相对于正常细胞[(0.5±0.06)g],干扰后细胞的肿瘤平均重量[(0.9±0.10)g]明显增大,体内成瘤性明显提高(t=2.5,P=0.01).结论磺基转移酶CHST13与肝癌细胞的侵袭、成瘤性密切相关,其有望成为肝癌临床治疗的新靶点.
목적 통과연구광기전이매CHST13재인간암고전이세포주MHCC97-H화인간암저전이세포주MHCC97-L중적차이표체,명학기여간암전이적상관성,종이증실간암전이진단급항종류치료신파점.방법 채용실시PCR、Western blot분석광기전이매CHST13재인간암고、저전이세포주적차이표체,통과RNA간우기술간예CHST13기인,검측간우전후MHCC97-L세포적체외침습력급체내성류성.결과 CHST13재인간암고、저전이세포주중표체구유명현차이;하조MHCC97-L세포중CHST13기인표체후,천과기저막적세포수량[(30±3)개]명현다우미처리조[(14±2)개],체외침습력현저증강(t=2.8,P=0.005);상대우정상세포[(0.5±0.06)g],간우후세포적종류평균중량[(0.9±0.10)g]명현증대,체내성류성명현제고(t=2.5,P=0.01).결론 광기전이매CHST13여간암세포적침습、성류성밀절상관,기유망성위간암림상치료적신파점.
Objective To clarify the association between chondroitin-4-O-sulfotransferase-3 (CHST13) and hepatoma metastasis by the research of differential expression of CHST13 in MHCC97-H and MHCC97-L human hepatocarcinoma cell lines,which have high and low metastatic potential,respectively,and to confirm novel target of hepatoma metastasis and anti-tumor therapy.Methods The differential expressions of CHST13 in MHCC97-H and MHCC97-L human hepatocarcinoma cell lines were analyzed by real-time PCR,Western blot.CHST13 was silenced using RNA interference approach to detect the invasive ability in vitro and tumorigenicity in vivo in MHCC97-L cells.Results The expression of CHST13 was different in MHCC97-L cells,as compared to those in MHCC97-H cells.Knockdown of CHST13 expression enhanced MHCC97-L cells invasion and tumorigenicity both in vitro (t =2.8,P =0.005) and in vivo (t =2.5,P =0.01).The quantity of cells which crossed basement membrane increased [(30 ± 3):(14 ± 2)],and the average weight of tumor increased [(0.9 ± 0.10) g:(0.5 ± 0.06) g].Conclusion The differential expressions of CHST13 in human hepatocarcinoma cell lines correlate with tumor invasion and tumorigenicity,and it is expected to be a novel target for the treatment of hepatocellular carcinoma.