国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2014年
5期
386-389
,共4页
徐华平%位玲霞%董延磊%张师前
徐華平%位玲霞%董延磊%張師前
서화평%위령하%동연뢰%장사전
卵巢肿瘤%RASSF1A基因%5-氮杂-2-脱氧胞苷
卵巢腫瘤%RASSF1A基因%5-氮雜-2-脫氧胞苷
란소종류%RASSF1A기인%5-담잡-2-탈양포감
Ovarian neoplasms%RASSF1A gene%5-aza-2'deoxycytidine
目的 观察5-氮杂-2'-脱氧胞苷(5-Aza-CdR)对体外培养的人卵巢癌细胞株(SKOV3、3AO) RASSF1A mRNA及蛋白表达的影响.方法 卵巢癌细胞株(SKOV3、3AO)经不同浓度(0.5、5、50 μmol/L)的5-Aza-CdR处理,未经处理的作为对照组,反转录聚合酶链反应(RT-PCR)及免疫印记(Western Blot)检测RASSF1A在mRNA和蛋白质水平表达的变化.结果 对照组细胞株SKOV3、3AO均可见RASSF1A mRNA及蛋白的表达,但表达较弱,经药物处理后的细胞RASSF1A mRNA及蛋白的表达较前明显增强.经不同浓度5-Aza-cdR处理的SKOV3细胞株中,随浓度增加,RASSF1A mRNA及蛋白的表达依次递增.经不同浓度5-Aza-cdR处理的3AO细胞株中,经0.5μmol/L 5-Aza-cdR处理后,RASSF1A mRNA的表达明显低于经5和50 μmol/L 5-Aza-cdR处理后的表达(t=-8.866,P=0.01;t=-12.256,P=0.000);但经5、50 μmol/L 5-Aza-cdR分别处理的3AO细胞株RASSF1A mRNA的表达差异无统计学意义(t=0.431,P=0.689).在3AO细胞株中,0.5 μmol/L组与5μmol/L组RASSF1A蛋白表达差异无统计学意义(t=-1.586,P=0.188).结论 经过5-Aza-CdR处理后,卵巢癌细胞株(SKOV3、3AO) RASSF1A mRNA及蛋白的表达均明显增强,细胞增殖能力受到明显抑制,从而起到抑制肿瘤细胞生长,甚至促进肿瘤细胞凋亡的作用.
目的 觀察5-氮雜-2'-脫氧胞苷(5-Aza-CdR)對體外培養的人卵巢癌細胞株(SKOV3、3AO) RASSF1A mRNA及蛋白錶達的影響.方法 卵巢癌細胞株(SKOV3、3AO)經不同濃度(0.5、5、50 μmol/L)的5-Aza-CdR處理,未經處理的作為對照組,反轉錄聚閤酶鏈反應(RT-PCR)及免疫印記(Western Blot)檢測RASSF1A在mRNA和蛋白質水平錶達的變化.結果 對照組細胞株SKOV3、3AO均可見RASSF1A mRNA及蛋白的錶達,但錶達較弱,經藥物處理後的細胞RASSF1A mRNA及蛋白的錶達較前明顯增彊.經不同濃度5-Aza-cdR處理的SKOV3細胞株中,隨濃度增加,RASSF1A mRNA及蛋白的錶達依次遞增.經不同濃度5-Aza-cdR處理的3AO細胞株中,經0.5μmol/L 5-Aza-cdR處理後,RASSF1A mRNA的錶達明顯低于經5和50 μmol/L 5-Aza-cdR處理後的錶達(t=-8.866,P=0.01;t=-12.256,P=0.000);但經5、50 μmol/L 5-Aza-cdR分彆處理的3AO細胞株RASSF1A mRNA的錶達差異無統計學意義(t=0.431,P=0.689).在3AO細胞株中,0.5 μmol/L組與5μmol/L組RASSF1A蛋白錶達差異無統計學意義(t=-1.586,P=0.188).結論 經過5-Aza-CdR處理後,卵巢癌細胞株(SKOV3、3AO) RASSF1A mRNA及蛋白的錶達均明顯增彊,細胞增殖能力受到明顯抑製,從而起到抑製腫瘤細胞生長,甚至促進腫瘤細胞凋亡的作用.
목적 관찰5-담잡-2'-탈양포감(5-Aza-CdR)대체외배양적인란소암세포주(SKOV3、3AO) RASSF1A mRNA급단백표체적영향.방법 란소암세포주(SKOV3、3AO)경불동농도(0.5、5、50 μmol/L)적5-Aza-CdR처리,미경처리적작위대조조,반전록취합매련반응(RT-PCR)급면역인기(Western Blot)검측RASSF1A재mRNA화단백질수평표체적변화.결과 대조조세포주SKOV3、3AO균가견RASSF1A mRNA급단백적표체,단표체교약,경약물처리후적세포RASSF1A mRNA급단백적표체교전명현증강.경불동농도5-Aza-cdR처리적SKOV3세포주중,수농도증가,RASSF1A mRNA급단백적표체의차체증.경불동농도5-Aza-cdR처리적3AO세포주중,경0.5μmol/L 5-Aza-cdR처리후,RASSF1A mRNA적표체명현저우경5화50 μmol/L 5-Aza-cdR처리후적표체(t=-8.866,P=0.01;t=-12.256,P=0.000);단경5、50 μmol/L 5-Aza-cdR분별처리적3AO세포주RASSF1A mRNA적표체차이무통계학의의(t=0.431,P=0.689).재3AO세포주중,0.5 μmol/L조여5μmol/L조RASSF1A단백표체차이무통계학의의(t=-1.586,P=0.188).결론 경과5-Aza-CdR처리후,란소암세포주(SKOV3、3AO) RASSF1A mRNA급단백적표체균명현증강,세포증식능력수도명현억제,종이기도억제종류세포생장,심지촉진종류세포조망적작용.
Objective To investigate the effect of 5-aza-2'deoxycytidine (5-Aza-CdR) on proliferation and expression of RASSF1 A gene in human ovarian cancer cell line SKOV3 and 3AO.Methods SKOV3 and 3AO cells were treated with different concentrations (0.5,5,50 μmol/L) of DNA methyltransferase inhibitor 5-Aza-CdR.RT-PCR and Western Blot were adopted to detect expression of mRNA and protein of RASSF1A gene before and after treatment with 5-Aza-CdR respectively.Results Compared with control group,when the 5-Aza-CdR concentration was 0.5,5,50 μmol/L after drug treatment,human ovarian cancer cells could significantly inhibit tumor cell growth; SKOV3 and 3AO cells in control group were observed weaker expression of RASSF1A mRNA.After treated with 5-Aza-CdR,the expressions of RASSF1A mRNA were observed increased with the increase of the drug concentration.After treated with different concentration of 5-Aza-CdR,the expressions of RASSF1A mRNA treated with 0.5 μmol/L 5-Aza-cdR was lower than those treated with 5 and 50 μmol/L 5-Aza-cdR (t =-8.866,P =0.01 ; t =-12.256,P =0.000).However,expressions of RASSF1A mRNA treated with 5 and 50 μmol/L 5-Aza-cdR respectively showed no statistical significance (t =0.431,P =0.689).Expressions of RASSF1A protein treated with 0.5 μmol/L 5-Aza-cdR and 5 μmol/L 5-Aza-cdR didn't show statistically significant (t =-1.586,P =0.188).Conclusion Expressions of RASSF1A mRNA and protein in SKOV3 and 3AO cells were evidently enhanced.As one kind of methyltransferases inhibitors,5-Aza-CdR can inhibit ovarian cancer cell line SKOV3,3AO growth through the RASSF1A promoter methylation,and thus promote their apoptosis.
