国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
1期
29-33
,共5页
红梅%陈秋红%刘有菊%王志瑾
紅梅%陳鞦紅%劉有菊%王誌瑾
홍매%진추홍%류유국%왕지근
藏药%玛奴西汤颗粒%急性毒性实验%长期毒性实验
藏藥%瑪奴西湯顆粒%急性毒性實驗%長期毒性實驗
장약%마노서탕과립%급성독성실험%장기독성실험
Tibetan medicine%MNXT granule%Acute toxicity%Long-term toxic test
目的 通过藏药玛奴西汤颗粒的急性毒性实验、长期毒性实验,观察其毒性反应,为临床安全用药提供依据.方法 急性毒性实验采用小鼠最大耐受量灌胃法,观察24 h内灌胃给药3次,给药后连续观察14d,记录小鼠的毒性反应及死亡情况;长期毒性实验以13.33 g/kg·d-1、6.667 g/kg·d-1、3.33 g/kg·d-1(临床日用量的100倍、50倍、25倍)每天灌胃大鼠,连续灌胃30d,空白组给予等量的蒸馏水.实验期间观察大鼠行为、外观、进食量、饮水量、体质量,测定给药30 d及停药15d大鼠血象和血液生化指标、主要脏器系数、解剖学、病理组织形态学.结果 测得藏药玛奴西汤颗粒最大耐受量为39g/kg(相当于临床剂量的300倍)无任何不良反应；长期毒性实验给药30d及停药15d大鼠的外观、行为、毛色无明显异常；摄食量、体重、血象指标、血液生化指标、主要脏器系数无明显异常；脏器病理学检查无明显药物性病变.结论藏药玛奴西汤颗粒对小鼠急性毒性实验、大鼠长期毒性实验无明显毒性作用.
目的 通過藏藥瑪奴西湯顆粒的急性毒性實驗、長期毒性實驗,觀察其毒性反應,為臨床安全用藥提供依據.方法 急性毒性實驗採用小鼠最大耐受量灌胃法,觀察24 h內灌胃給藥3次,給藥後連續觀察14d,記錄小鼠的毒性反應及死亡情況;長期毒性實驗以13.33 g/kg·d-1、6.667 g/kg·d-1、3.33 g/kg·d-1(臨床日用量的100倍、50倍、25倍)每天灌胃大鼠,連續灌胃30d,空白組給予等量的蒸餾水.實驗期間觀察大鼠行為、外觀、進食量、飲水量、體質量,測定給藥30 d及停藥15d大鼠血象和血液生化指標、主要髒器繫數、解剖學、病理組織形態學.結果 測得藏藥瑪奴西湯顆粒最大耐受量為39g/kg(相噹于臨床劑量的300倍)無任何不良反應；長期毒性實驗給藥30d及停藥15d大鼠的外觀、行為、毛色無明顯異常；攝食量、體重、血象指標、血液生化指標、主要髒器繫數無明顯異常；髒器病理學檢查無明顯藥物性病變.結論藏藥瑪奴西湯顆粒對小鼠急性毒性實驗、大鼠長期毒性實驗無明顯毒性作用.
목적 통과장약마노서탕과립적급성독성실험、장기독성실험,관찰기독성반응,위림상안전용약제공의거.방법 급성독성실험채용소서최대내수량관위법,관찰24 h내관위급약3차,급약후련속관찰14d,기록소서적독성반응급사망정황;장기독성실험이13.33 g/kg·d-1、6.667 g/kg·d-1、3.33 g/kg·d-1(림상일용량적100배、50배、25배)매천관위대서,련속관위30d,공백조급여등량적증류수.실험기간관찰대서행위、외관、진식량、음수량、체질량,측정급약30 d급정약15d대서혈상화혈액생화지표、주요장기계수、해부학、병리조직형태학.결과 측득장약마노서탕과립최대내수량위39g/kg(상당우림상제량적300배)무임하불량반응；장기독성실험급약30d급정약15d대서적외관、행위、모색무명현이상；섭식량、체중、혈상지표、혈액생화지표、주요장기계수무명현이상；장기병이학검사무명현약물성병변.결론장약마노서탕과립대소서급성독성실험、대서장기독성실험무명현독성작용.
Objective The Toxicology study of Tibetan medicine MNXT granules was observed to provide basis for clinical safe medication.Methods The acute toxicity test in mice was conducted with the oral maximum-tolerated dosage,and then toxicity reaction and death situations in mice at 14d after the intragastric administration (ig) one day at 3 times was observed; Long-term toxic test:the does of MNXT granule 13.23 g/kg · d-1,6.667 g/kg· d-1,3.33 g/kg· d-1 (equivalent 100,50,25 times of the clinical dosage) were continuous administered to medicating groups for 30 days,and blank group was given distilled water instead.The rats'behavior,appearance,food intake,water intake,body weight were observed,and the blood,blood biochemical parameters,the main organ coefficient,anatomical,pathological morphology were determined at 30d after administration and 15d after withdrawal.Results The maximum study medication dose of Tibet an medicine MNXT granule was 39 g/kg (equivalent to 300 times the clinical dose) and the mice had not any adverse reaction.Long-term toxicity test:the rats' blood and blood biochemical parameters,the main organ coefficient,anatomical,pathological morphology had not significant differences compared with the blank grou? during the 30d administration and 15d withdrawal.Conclusion Toxicity of the Tibetan medicine MNXT granules was not observed in acute or long-term toxicity test.
