国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
1期
34-36
,共3页
曾立%向荣%傅春燕%尹文清
曾立%嚮榮%傅春燕%尹文清
증립%향영%부춘연%윤문청
定心藤%挥发油%四甲基偶氮唑盐法%抑制作用
定心籐%揮髮油%四甲基偶氮唑鹽法%抑製作用
정심등%휘발유%사갑기우담서염법%억제작용
Mappianthus iodoies%volatile oil%MTT%Inhibition
目的 探讨瑶药定心藤挥发油对肺腺癌细胞(SPC-A-1)、肝癌细胞(BEL-7402)的抑制作用.方法 超临界CO2法提取挥发油,以半数抑制浓度(IC50)为评价抗肿瘤活性强度的指标,采用四甲基偶氮唑盐法(MTT法)观察定心藤挥发油对肿瘤细胞的抑制作用.结果 定心藤挥发油在浓度为200 μg/ml时,其抑制率均＞50％,IC5o分别为169.54、695.21 μg/ml.结论 瑶药定心藤超临界CO2提取挥发油对两种肿瘤细胞均有抑制作用.
目的 探討瑤藥定心籐揮髮油對肺腺癌細胞(SPC-A-1)、肝癌細胞(BEL-7402)的抑製作用.方法 超臨界CO2法提取揮髮油,以半數抑製濃度(IC50)為評價抗腫瘤活性彊度的指標,採用四甲基偶氮唑鹽法(MTT法)觀察定心籐揮髮油對腫瘤細胞的抑製作用.結果 定心籐揮髮油在濃度為200 μg/ml時,其抑製率均＞50％,IC5o分彆為169.54、695.21 μg/ml.結論 瑤藥定心籐超臨界CO2提取揮髮油對兩種腫瘤細胞均有抑製作用.
목적 탐토요약정심등휘발유대폐선암세포(SPC-A-1)、간암세포(BEL-7402)적억제작용.방법 초림계CO2법제취휘발유,이반수억제농도(IC50)위평개항종류활성강도적지표,채용사갑기우담서염법(MTT법)관찰정심등휘발유대종류세포적억제작용.결과 정심등휘발유재농도위200 μg/ml시,기억제솔균＞50％,IC5o분별위169.54、695.21 μg/ml.결론 요약정심등초림계CO2제취휘발유대량충종류세포균유억제작용.
Objective To study the inhibition activity of volatile oil from Mappianthus iodoies on SPC-A-1 and BEL-7402 cancer cells.Methods The volatile oil in Mappianthus iodoie was extracted by SFE-CO2.MTT assay was employed to test the antitumor effect of volatile oil from Mappianthus iodoies in two kinds of malignant tumor cell lines,with IC50 applied to evaluate the degree of inhibition activity.Results When the dose of volatile oil from Mappianthus iodoies was 200 μg/ml,the inhibition ratios of the tumor cell was in excess of 50％,the IC50 was 169.54,695.21 μg/ml respectively.Conclusion Volatile oil from Mappianthus iodoies extracted by SFE-CO2 has obvious inhibition activity on SPC-A-1 and BEL-7402 cancer cells.
