国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
1期
46-48
,共3页
飞扬草%杨梅苷%槲皮苷%反相高效液相色谱法
飛颺草%楊梅苷%槲皮苷%反相高效液相色譜法
비양초%양매감%곡피감%반상고효액상색보법
Euphorbia hirta L.%Myricetrin%Quercitroside%RP-HPLC
目的 采用反相高效液相色谱法(RP-HPLC)分析飞扬草中有效成分杨梅苷和槲皮苷的含量.方法 以ZORBAX SB-C18 (250 mm× 4.6 mm,5μm)色谱柱,以乙腈-0.1％磷酸溶液(21∶79)作为流动相,流速为0.8 ml/min,柱温:30℃；检测波长:256nm.结果 杨梅苷和槲皮苷分别在0.013～0.26 mg/ml、0.008～0.16 mg/ml范围内有良好的线性关系,平均加样回收率分别为99.1％、98.9％,RSD分别为0.91％、1.55％.结论 本法准确、简便、重复性好,可用于飞扬草中杨梅苷和槲皮苷的定量分析.
目的 採用反相高效液相色譜法(RP-HPLC)分析飛颺草中有效成分楊梅苷和槲皮苷的含量.方法 以ZORBAX SB-C18 (250 mm× 4.6 mm,5μm)色譜柱,以乙腈-0.1％燐痠溶液(21∶79)作為流動相,流速為0.8 ml/min,柱溫:30℃；檢測波長:256nm.結果 楊梅苷和槲皮苷分彆在0.013～0.26 mg/ml、0.008～0.16 mg/ml範圍內有良好的線性關繫,平均加樣迴收率分彆為99.1％、98.9％,RSD分彆為0.91％、1.55％.結論 本法準確、簡便、重複性好,可用于飛颺草中楊梅苷和槲皮苷的定量分析.
목적 채용반상고효액상색보법(RP-HPLC)분석비양초중유효성분양매감화곡피감적함량.방법 이ZORBAX SB-C18 (250 mm× 4.6 mm,5μm)색보주,이을정-0.1％린산용액(21∶79)작위류동상,류속위0.8 ml/min,주온:30℃；검측파장:256nm.결과 양매감화곡피감분별재0.013～0.26 mg/ml、0.008～0.16 mg/ml범위내유량호적선성관계,평균가양회수솔분별위99.1％、98.9％,RSD분별위0.91％、1.55％.결론 본법준학、간편、중복성호,가용우비양초중양매감화곡피감적정량분석.
Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Euphorbia Hirta L.Methods The ZORBAX SB-C18 (250 mm × 4.6 mm,5 μn) column was used,the mobile phase consisted of acetonitrile:0.1％ H3PO4(21 ∶ 79),the flow rate was 0.8 ml/min,the column temperature was 30℃ the detecting wavelength was at 256 nm.Results The cablibration curve was linear within a range of 0.013～0.26 mg/ml and 0.008～0.16 mg/ml,the average recovery was 99.1％,98.9％and the RSD was 0.91％,1.55％,respectively.Conclusion The method is simple,repeatable and accurate,it can be applied in quantitative determination of myricetrin and quercitroside in Euphorbia Hirta L..
