CAJ | 학술논문

目的研究化胃舒颗粒对顺铂所致Beagle犬呕吐模型的影响及其作用机理.方法以Beagle犬顺铂呕吐模型为研究对象,观察化胃舒颗粒对该模型的Beagle犬呕吐潜伏期、呕吐次数、血清中胃动素的含量、延髓及回肠中5-HT、P物质含量的影响.结果 ①呕吐潜伏期:化胃舒颗粒低、中、高剂量组呕吐潜伏期分别为(137.3±53.4) min、(122.8±50.7) min、(116.8±44.6) min,与模型组(60.8±37.1) min比较,差异均有统计学意义(P＜0.05).②呕吐次数:与模型组呕吐次数[(270.3±51.8)次]比较,化胃舒颗粒低、中剂量组呕吐次数[分别为(111.5±45.0)次、(149.5±26.8)次],均减少(P均＜0.05).③血清中胃动素含量:化胃舒颗粒低剂量组血清中胃动素含量为(0.230±0.074) ng/ml,化胃舒颗粒中、高剂量组为(0.235±0.071) ng/ml、(0.245±0.062) ng/ml,均低于模型组[(0.354±0.098) ng/ml,P均＜0.05].④延髓及回肠中5-HT:化胃舒颗粒低、中、高剂量组延髓5-HT含量分别为(1.620±0.329) ng/ml、(1.194±0.386) ng/ml、(1.269±0.251) ng/ml,均低于模型组[(2.028±0.198) ng/ml,P均＜0.05].化胃舒颗粒低、中、高剂量组回肠5-HT含量为分别为(1.108±0.291) ng/ml、(1.194±0.386) ng/ml、(1.269±0.251) ng/ml,均低于模型组[(1.634±0.221) ng/ml,P均＜0.05].⑤P物质含量:模型组回肠P物质含量(0.356±0.063) ng/ml,化胃舒颗粒低剂量组为(0.274±0.064) ng/ml,低于模型组(P＜0.05).模型组延髓P物质含量(0.432±0.021) ng/ml,化胃舒颗粒低、中、高剂量组延髓P物质含量分别为(0.370±0.040) ng/ml、(0.385±0.029) ng/ml、(0.386±0.041) ng/ml,均低于模型组(P均＜0.05).结论化胃舒颗粒可防治顺铂引起的Beagle犬呕吐,具有止呕功效.
목적 연구화위서과립대순박소치Beagle견구토모형적영향급기작용궤리.방법 이Beagle견순박구토모형위연구대상,관찰화위서과립대해모형적Beagle견구토잠복기、구토차수、혈청중위동소적함량、연수급회장중5-HT、P물질함량적영향.결과 ①구토잠복기:화위서과립저、중、고제량조구토잠복기분별위(137.3±53.4) min、(122.8±50.7) min、(116.8±44.6) min,여모형조(60.8±37.1) min비교,차이균유통계학의의(P＜0.05).②구토차수:여모형조구토차수[(270.3±51.8)차]비교,화위서과립저、중제량조구토차수[분별위(111.5±45.0)차、(149.5±26.8)차],균감소(P균＜0.05).③혈청중위동소함량:화위서과립저제량조혈청중위동소함량위(0.230±0.074) ng/ml,화위서과립중、고제량조위(0.235±0.071) ng/ml、(0.245±0.062) ng/ml,균저우모형조[(0.354±0.098) ng/ml,P균＜0.05].④연수급회장중5-HT:화위서과립저、중、고제량조연수5-HT함량분별위(1.620±0.329) ng/ml、(1.194±0.386) ng/ml、(1.269±0.251) ng/ml,균저우모형조[(2.028±0.198) ng/ml,P균＜0.05].화위서과립저、중、고제량조회장5-HT함량위분별위(1.108±0.291) ng/ml、(1.194±0.386) ng/ml、(1.269±0.251) ng/ml,균저우모형조[(1.634±0.221) ng/ml,P균＜0.05].⑤P물질함량:모형조회장P물질함량(0.356±0.063) ng/ml,화위서과립저제량조위(0.274±0.064) ng/ml,저우모형조(P＜0.05).모형조연수P물질함량(0.432±0.021) ng/ml,화위서과립저、중、고제량조연수P물질함량분별위(0.370±0.040) ng/ml、(0.385±0.029) ng/ml、(0.386±0.041) ng/ml,균저우모형조(P균＜0.05).결론 화위서과립가방치순박인기적Beagle견구토,구유지구공효.
Objective To study on the effects of Huaweishu granule on cisplatin-induced Beagle dog vomiting models and its mechanism.Methods Cisplatin-induced Beagle dog vomiting models were adopted as research object.The changes of vomiting frequency,latency,serum motilin content in the medulla oblongata and ileum,5-HT and substance P content of these models after using Hua weishu Granule were observed.Results ① Latent period of vomiting of the model group was (60.8±37.1)min; while this period was (137.3± 53.4)min,(122.8 ± 50.7) min,(l16.8±44.6)min,in the Huawei-shu low,medium and high dose group respectively,all showing a statistic difference than the model group (P＜0.05).②Frequency of vomiting in the model group was (270.3±51.8),while this frequence was (111.5±45.0) and (149.5±26.8) in Huawei-shu low and medium dose group respectively; ③ serum motilin in the model group was (0.354±0.098)ng/ml,while serum motilin in Huawei-shu low,medium and high dose group was (0.230±0.074) ng/ml,(0.235± 0.071) ng/ml,and (0.245± 0.062)ng/ml respectively,all lower than the model group (P＜ 0.05).④ Medulla oblongata 5-HT in the model group was (2.028 ±0.198)ng/ml,while medulla oblongata 5-HT in Huawei-shu low,medium and high dose group was (1.620±0.329)ng/ml,(1.194±0.386)ng/ml,and (1.269 ± 0.251) ng/ml respectively,all were lower than the model group (P＜0.05); ileum 5-HT in the model group was (1.634± 0.221)ng/ml,while ileum 5-HT in Huawei-shu low,medium and high dose group was (1.108±0.291)ng/ml,(1.194±0.386)ng/ml,and (1.269 ± 0.251) ng/ml respectively,all were lower than the model group(P＜0.05) ;⑤ ileal substance P content of the model group was (0.356±0.063)ng/ml,while ildeal P content in Huawei-shu low dose group was (0.274±0.064)ng/ml,lower than the model group than(P＜0.05).The medullary substance P content was (0.432±0.021)ng/ml in the model group,while medullary P content in Huawei-shu low,medium and high dose group was (0.370±0.040) ng/ml,(0.385±0.029) ng/ml,and (0.386± 0.041)ng/ml respectively,all were lower than the model group(P＜0.05).Conclusion Huawei-shu granule can prevent cisplatin-induced Beagle vomiting.