国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
2期
129-132
,共4页
张建国%杨光%石炳娟%李丽%周建民
張建國%楊光%石炳娟%李麗%週建民
장건국%양광%석병연%리려%주건민
中药组方%微循环%药理作用%压力性溃疡
中藥組方%微循環%藥理作用%壓力性潰瘍
중약조방%미순배%약리작용%압력성궤양
Medicines party%Microcirculation%Pharmacological action%Pressure ulcer
目的 观察压力性溃疡中药组方对大鼠微循环的影响.方法 大鼠采用随机数字表法分为中药组方高剂量组、中药组方低剂量组、阳性对照组和阴性对照组.肠系膜局部滴加肾上腺素液造成大鼠肠系膜微循环障碍模型,考察各组受试大鼠的细动脉管径、细动脉流速、毛细血管网交点计数等指标.结果 各时段大鼠肠系膜细动脉管径变化,阳性对照组分别为(74.73±4.23) min、(73.46±4.02) min、(81.60±3.23) min、(93.37±3.84)min、(98.38±3.25) min,中药组方高剂量组分别为(72.26±2.90) min、(72.16±3.20) min、(82.45±2.85) min、(94.64±3.37) min,两组比较无显著性差异(P＞0.05).各时段大鼠肠系膜细动脉流速变化,阴性对照组分别为(5.06±1.12) min、(3.47±0.97) min、(8.51±1.15)min、(43.55±3.24) min、(56.47±3.54) min,阳性对照组分别为(19.37±1.81)min、(21.54±2.69) min、(39.86±3.53) min、(86.41±4.27) min、(94.35±4.23) min,中药组方高剂量组分别为(13.73±2.02) min、(22.67±2.48) min、(46.32±4.27) min、(89.18±5.13)min、(95.07±4.67) min,中药组方低剂量组分别为(6.22±1.48) min、(10.34±2.09) min、(25.41±2.49) min、(56.87±3.75) min、(74.30±3.18)min,阳性对照组和中药组方高剂量组大鼠肠系膜细动脉流速9min均恢复到滴加肾上腺素前水平(P＞0.05),阳性对照组、中药组方高剂量组与中药组方低剂量组、阴性对照组各时段数据比较有显著差异(P＜0.05).各时段大鼠肠系膜毛细血管网交点计数变化,阴性对照组分别为0、16.92±1.17、39.19±2.65,阳性对照组分别为20.66±1.89、36.78±3.04、76.04±4.29,中药组方高剂量组分别为14.33±1.46、43.75±3.74、81.23±3.34,中药组方低剂量组分别为9.50±1.13、23.19±2.44、55.62±3.46,中药组方高剂量组、中药组方低剂量组较阴性对照组毛细血管网交点计数增多(P＜0.05),中药组方高剂量组优于阳性对照组(P＜0.05).局部微循环血流恢复时间,阳性对照组为(8.23±2.61)min,中药组方高剂量组为(8.56±1.87)min,作用相当(P＞0.05).结论 中药组方改善肾上腺素所致大鼠肠系膜微循环障碍,作用呈量效正比关系.
目的 觀察壓力性潰瘍中藥組方對大鼠微循環的影響.方法 大鼠採用隨機數字錶法分為中藥組方高劑量組、中藥組方低劑量組、暘性對照組和陰性對照組.腸繫膜跼部滴加腎上腺素液造成大鼠腸繫膜微循環障礙模型,攷察各組受試大鼠的細動脈管徑、細動脈流速、毛細血管網交點計數等指標.結果 各時段大鼠腸繫膜細動脈管徑變化,暘性對照組分彆為(74.73±4.23) min、(73.46±4.02) min、(81.60±3.23) min、(93.37±3.84)min、(98.38±3.25) min,中藥組方高劑量組分彆為(72.26±2.90) min、(72.16±3.20) min、(82.45±2.85) min、(94.64±3.37) min,兩組比較無顯著性差異(P＞0.05).各時段大鼠腸繫膜細動脈流速變化,陰性對照組分彆為(5.06±1.12) min、(3.47±0.97) min、(8.51±1.15)min、(43.55±3.24) min、(56.47±3.54) min,暘性對照組分彆為(19.37±1.81)min、(21.54±2.69) min、(39.86±3.53) min、(86.41±4.27) min、(94.35±4.23) min,中藥組方高劑量組分彆為(13.73±2.02) min、(22.67±2.48) min、(46.32±4.27) min、(89.18±5.13)min、(95.07±4.67) min,中藥組方低劑量組分彆為(6.22±1.48) min、(10.34±2.09) min、(25.41±2.49) min、(56.87±3.75) min、(74.30±3.18)min,暘性對照組和中藥組方高劑量組大鼠腸繫膜細動脈流速9min均恢複到滴加腎上腺素前水平(P＞0.05),暘性對照組、中藥組方高劑量組與中藥組方低劑量組、陰性對照組各時段數據比較有顯著差異(P＜0.05).各時段大鼠腸繫膜毛細血管網交點計數變化,陰性對照組分彆為0、16.92±1.17、39.19±2.65,暘性對照組分彆為20.66±1.89、36.78±3.04、76.04±4.29,中藥組方高劑量組分彆為14.33±1.46、43.75±3.74、81.23±3.34,中藥組方低劑量組分彆為9.50±1.13、23.19±2.44、55.62±3.46,中藥組方高劑量組、中藥組方低劑量組較陰性對照組毛細血管網交點計數增多(P＜0.05),中藥組方高劑量組優于暘性對照組(P＜0.05).跼部微循環血流恢複時間,暘性對照組為(8.23±2.61)min,中藥組方高劑量組為(8.56±1.87)min,作用相噹(P＞0.05).結論 中藥組方改善腎上腺素所緻大鼠腸繫膜微循環障礙,作用呈量效正比關繫.
목적 관찰압력성궤양중약조방대대서미순배적영향.방법 대서채용수궤수자표법분위중약조방고제량조、중약조방저제량조、양성대조조화음성대조조.장계막국부적가신상선소액조성대서장계막미순배장애모형,고찰각조수시대서적세동맥관경、세동맥류속、모세혈관망교점계수등지표.결과 각시단대서장계막세동맥관경변화,양성대조조분별위(74.73±4.23) min、(73.46±4.02) min、(81.60±3.23) min、(93.37±3.84)min、(98.38±3.25) min,중약조방고제량조분별위(72.26±2.90) min、(72.16±3.20) min、(82.45±2.85) min、(94.64±3.37) min,량조비교무현저성차이(P＞0.05).각시단대서장계막세동맥류속변화,음성대조조분별위(5.06±1.12) min、(3.47±0.97) min、(8.51±1.15)min、(43.55±3.24) min、(56.47±3.54) min,양성대조조분별위(19.37±1.81)min、(21.54±2.69) min、(39.86±3.53) min、(86.41±4.27) min、(94.35±4.23) min,중약조방고제량조분별위(13.73±2.02) min、(22.67±2.48) min、(46.32±4.27) min、(89.18±5.13)min、(95.07±4.67) min,중약조방저제량조분별위(6.22±1.48) min、(10.34±2.09) min、(25.41±2.49) min、(56.87±3.75) min、(74.30±3.18)min,양성대조조화중약조방고제량조대서장계막세동맥류속9min균회복도적가신상선소전수평(P＞0.05),양성대조조、중약조방고제량조여중약조방저제량조、음성대조조각시단수거비교유현저차이(P＜0.05).각시단대서장계막모세혈관망교점계수변화,음성대조조분별위0、16.92±1.17、39.19±2.65,양성대조조분별위20.66±1.89、36.78±3.04、76.04±4.29,중약조방고제량조분별위14.33±1.46、43.75±3.74、81.23±3.34,중약조방저제량조분별위9.50±1.13、23.19±2.44、55.62±3.46,중약조방고제량조、중약조방저제량조교음성대조조모세혈관망교점계수증다(P＜0.05),중약조방고제량조우우양성대조조(P＜0.05).국부미순배혈류회복시간,양성대조조위(8.23±2.61)min,중약조방고제량조위(8.56±1.87)min,작용상당(P＞0.05).결론 중약조방개선신상선소소치대서장계막미순배장애,작용정량효정비관계.
Objective To observe the effect of traditional Chinese treating pressure ulcer compound on microcirculation of rats in-vivo.Methods Rats were divided into four groups according to the random number table,includeing high-dose group,low-dose group,positive control group and negative control group.Model rats of microcirculation disturbance were created by dropping epinephrine on partial of mesentery,and then the following indexes of four groups were detected: arteriole diameter,arteriole flow rate,junction of capillary network counts,etc.Results The rats mesentery arteriole diameter variation of positive control group in different time were(74.73±4.23)min,(73.46±4.02)min,(81.60±3.23)min,(93.37±3.84)min,(98.38±3.25) min respectively,and the variation of high-dose group in different time were (72.26± 2.90)min,(72.16 ± 3.20) min,(82.45±2.85)min,(94.64 ± 3.37)min respectively.There was no significant difference between the two groups (P＞0.05).The rats mesentery arteriole flow rate variation of negative control group in different time were (5.06±1.12)min,(3.47±0.97)min,(8.51±1.15)min,(43.55±3.24)min,(56.47±3.54)min respectively,the variation of positive control group were (19.37± 1.81)min,(21.54±2.69) min,(39.86±3.53)min,(86.41 ±4.27) min,(94.35 ± 4.23) min respectively,the variation of high-dose group were (13.73 ± 2.02) min,(22.67 ± 2.48) min,(46.32 ± 4.27) min,(89.18 ± 5.13) min,(95.07 ± 4.67) min respectively,and the variation of low-dose group were (6.22±1.48)min,(10.34±2.09)min,(25.41±2.49)min,(56.87±3.75)min,(74.30±3.18)min respectively.The rats mesentery arteriole flow rate has recovered to the level of before dropping epinephrine by 9 rmin in positive control group and high-dose group,and there were no significant difference between the two groups (P＞0.05),but there were statistical significance when high-dose group and positive control group were compared with low-dose group and negative control group (P＜0.05).The junction of capillary network counts variation in different time were: negative control group 0,16.92± 1.17,39.19±2.65,positive control group 20.66±1.89,36.78±3.04,76.04±4.29,high-dose group 14.33±1.46,43.75±3.74,81.23±3.34,low-dose group 9.50± 1.13,23.19 ± 2.44,55.62 ± 3.46,respectively,the junction of capillary network counts of high-dose group and low-dose group were more than negative control group (P＜ 0.05),and the high-dose group was more than the positive control group (P＜0.05).The resuming time of local microcirculation flow of positive control group (8.23 ±2.61)rmin was relatively equivalent to high-dose group (8.56 ±1.87) min,(P＞0.05).Conclusion Traditional Chinese compound can obviously modify microcirculation disturbance of rats mesentery caused by epinephrine.Its effect showed a dose-effect proportional relationship.
