中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2012年
9期
992-996
,共5页
鲍磊%沈华%张铮%徐英%马明洲%程惠%宋希%秦海东
鮑磊%瀋華%張錚%徐英%馬明洲%程惠%宋希%秦海東
포뢰%침화%장쟁%서영%마명주%정혜%송희%진해동
参附注射液%脑%缺血-再灌注损伤%凋亡%血红素氧合酶-1
參附註射液%腦%缺血-再灌註損傷%凋亡%血紅素氧閤酶-1
삼부주사액%뇌%결혈-재관주손상%조망%혈홍소양합매-1
Shenfu injection%Brain%Ischemia reperfusion injury%Apoptosis%Heme oxygenase-1
目的 探讨参附注射液(Shenfu injection,SF)对大鼠局灶性脑缺血-再灌注损伤(cerebral ischemia reperfusion injury,CIRI)中脑神经元细胞凋亡及血红素氧合酶-1(heme oxygenase-1,HO-1)表达的影响,并探讨其可能的机制.方法 SD雄性大鼠42只,随机(随机数字法)分为假手术组(Sham组)、缺血一再灌注组(IR组)、参附注射液组(SF组),制造大脑中动脉阻塞模型,缺血2h后再灌注3h、6h.SF组于再灌注时腹腔注射参附注射液10ml/kg.光镜和电镜下观察脑组织神经细胞形态学变化,用Annexin-V-PI双染法通过流式细胞仪检测脑织凋亡细胞并计算凋亡率,应用RT-PCR技术检测HO-1.结果 IR组与Sham组相比,3、6h时点脑组织凋亡率明显增加(P<0.01).SF组与IR组相比,SF组3、6h时点脑织凋亡率明显减少,差异均具有统计学意义(P<O.O1),3、6h时点脑组织HO-1的表达显著增加(P<0.01),脑组织结构损害明显减轻.结论 早期应用参附注射液可以诱导HO-1大量表达从而抑制细胞凋亡的发生,进而减轻脑组织损伤.
目的 探討參附註射液(Shenfu injection,SF)對大鼠跼竈性腦缺血-再灌註損傷(cerebral ischemia reperfusion injury,CIRI)中腦神經元細胞凋亡及血紅素氧閤酶-1(heme oxygenase-1,HO-1)錶達的影響,併探討其可能的機製.方法 SD雄性大鼠42隻,隨機(隨機數字法)分為假手術組(Sham組)、缺血一再灌註組(IR組)、參附註射液組(SF組),製造大腦中動脈阻塞模型,缺血2h後再灌註3h、6h.SF組于再灌註時腹腔註射參附註射液10ml/kg.光鏡和電鏡下觀察腦組織神經細胞形態學變化,用Annexin-V-PI雙染法通過流式細胞儀檢測腦織凋亡細胞併計算凋亡率,應用RT-PCR技術檢測HO-1.結果 IR組與Sham組相比,3、6h時點腦組織凋亡率明顯增加(P<0.01).SF組與IR組相比,SF組3、6h時點腦織凋亡率明顯減少,差異均具有統計學意義(P<O.O1),3、6h時點腦組織HO-1的錶達顯著增加(P<0.01),腦組織結構損害明顯減輕.結論 早期應用參附註射液可以誘導HO-1大量錶達從而抑製細胞凋亡的髮生,進而減輕腦組織損傷.
목적 탐토삼부주사액(Shenfu injection,SF)대대서국조성뇌결혈-재관주손상(cerebral ischemia reperfusion injury,CIRI)중뇌신경원세포조망급혈홍소양합매-1(heme oxygenase-1,HO-1)표체적영향,병탐토기가능적궤제.방법 SD웅성대서42지,수궤(수궤수자법)분위가수술조(Sham조)、결혈일재관주조(IR조)、삼부주사액조(SF조),제조대뇌중동맥조새모형,결혈2h후재관주3h、6h.SF조우재관주시복강주사삼부주사액10ml/kg.광경화전경하관찰뇌조직신경세포형태학변화,용Annexin-V-PI쌍염법통과류식세포의검측뇌직조망세포병계산조망솔,응용RT-PCR기술검측HO-1.결과 IR조여Sham조상비,3、6h시점뇌조직조망솔명현증가(P<0.01).SF조여IR조상비,SF조3、6h시점뇌직조망솔명현감소,차이균구유통계학의의(P<O.O1),3、6h시점뇌조직HO-1적표체현저증가(P<0.01),뇌조직결구손해명현감경.결론 조기응용삼부주사액가이유도HO-1대량표체종이억제세포조망적발생,진이감경뇌조직손상.
Objective To investigate the effects of Shenfu injection ( SF,a Chinese herbal medicine preparation made of Codonopsis pilosula and Aconitum carmichaeli) on the cell apoptosis of focal cerebral ischemic-reperfusion injured rats and the expression of heme oxygenase-1 (HO-1). Methods Forty-two male Sprague-Dawley rats used for producing unilateral brain ischemia reperfusion model were randomly divided into three groups:sham operation group ( Sham group),ischemia reperfusion group ( IR group),and SF Injection group (SF group).The model of focal cerebral ischemia-reperfusion injury was induced by transient occlusion of middle cerebral artery (ischemia for 2 h,and reperfusion for 3,6 h respectively).In SF group,SF ( 10 mg/kg) was intraperitoneally injected duri(n)g reperfusion.Cell apoptosis rate in brain tissue was detected by the technique of Annexin-V-PI double staining and was counted in flow cytometer.Expression of HO-1 in brain was measured by RT-PCR,while the pathological and ultra structure changes of cerebral tissue were also observed.Results Cell apoptosis rate of brain tissue were significantly higher in IR group than that in Sham group (P <0.01 ),while SF group had less significant changes in cell apoptosis rate, HO-1 level of brain tissue than IR group (P < O.01 ).The ultra structure change of brain tissue was less in SF group than that in IR group.Conclusions During early stage of brain IR injury,SF inhibits cellular apoptosis and in turn protects the brain from injury which is attributed to the increase in HO-1 expression induced by SF.