中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2013年
7期
741-743
,共3页
许竞艳%严敏%陈钢%郁丽娜
許競豔%嚴敏%陳鋼%鬱麗娜
허경염%엄민%진강%욱려나
血必净注射液%细胞凋亡%脑损伤
血必淨註射液%細胞凋亡%腦損傷
혈필정주사액%세포조망%뇌손상
Xuebijing injection%Apoptosis%Brain injury
目的 评价血必净对兔创伤性脑损伤脑细胞凋亡的影响.方法 雄性健康新西兰大耳白兔36只,4～5个月龄,体质量2.1 ～2.5 kg,采用随机(随机数字法)数字表法将其随机分为三组(n=12):假手术组(S组)、损伤组(T组)和血必净治疗组(X组).T、X组采用改良Feeney法建立创伤性脑损伤模型,S组只钻出骨窗不打击脑组织.T、X组在制模成功后24 h采集兔损伤部位脑组织标本,S组于相同时间点采集兔对应部位脑组织标本.采用TUNEL法检测脑细胞凋亡情况,Western blot法检测脑组织Bcl-2、Bax蛋白表达.结果 与S组比较,T、X组脑细胞凋亡率升高,Bcl-2/Bax蛋白表达比值降低,差异具有统计学意义(P＜0.01)；与T组比较,X组脑细胞凋亡率降低,Bcl-2/Bax蛋白表达比值升高,差异具有统计学意义(P＜0.01).结论 血必净可一定程度降低兔创伤性脑损伤脑细胞的凋亡,从而发挥脑保护作用,其机制可能与调节Bcl-2/Bax蛋白功能向抗凋亡方向转移有关.
目的 評價血必淨對兔創傷性腦損傷腦細胞凋亡的影響.方法 雄性健康新西蘭大耳白兔36隻,4～5箇月齡,體質量2.1 ～2.5 kg,採用隨機(隨機數字法)數字錶法將其隨機分為三組(n=12):假手術組(S組)、損傷組(T組)和血必淨治療組(X組).T、X組採用改良Feeney法建立創傷性腦損傷模型,S組隻鑽齣骨窗不打擊腦組織.T、X組在製模成功後24 h採集兔損傷部位腦組織標本,S組于相同時間點採集兔對應部位腦組織標本.採用TUNEL法檢測腦細胞凋亡情況,Western blot法檢測腦組織Bcl-2、Bax蛋白錶達.結果 與S組比較,T、X組腦細胞凋亡率升高,Bcl-2/Bax蛋白錶達比值降低,差異具有統計學意義(P＜0.01)；與T組比較,X組腦細胞凋亡率降低,Bcl-2/Bax蛋白錶達比值升高,差異具有統計學意義(P＜0.01).結論 血必淨可一定程度降低兔創傷性腦損傷腦細胞的凋亡,從而髮揮腦保護作用,其機製可能與調節Bcl-2/Bax蛋白功能嚮抗凋亡方嚮轉移有關.
목적 평개혈필정대토창상성뇌손상뇌세포조망적영향.방법 웅성건강신서란대이백토36지,4～5개월령,체질량2.1 ～2.5 kg,채용수궤(수궤수자법)수자표법장기수궤분위삼조(n=12):가수술조(S조)、손상조(T조)화혈필정치료조(X조).T、X조채용개량Feeney법건립창상성뇌손상모형,S조지찬출골창불타격뇌조직.T、X조재제모성공후24 h채집토손상부위뇌조직표본,S조우상동시간점채집토대응부위뇌조직표본.채용TUNEL법검측뇌세포조망정황,Western blot법검측뇌조직Bcl-2、Bax단백표체.결과 여S조비교,T、X조뇌세포조망솔승고,Bcl-2/Bax단백표체비치강저,차이구유통계학의의(P＜0.01)；여T조비교,X조뇌세포조망솔강저,Bcl-2/Bax단백표체비치승고,차이구유통계학의의(P＜0.01).결론 혈필정가일정정도강저토창상성뇌손상뇌세포적조망,종이발휘뇌보호작용,기궤제가능여조절Bcl-2/Bax단백공능향항조망방향전이유관.
Objective To evaluate the effects of Xuebijing on apoptosis induced by traumatic brain injury in rabbits.Methods A total of 36 healthy adult male rabbits were randomly devided into 3 groups (n =12):sham group (group S),traumatic brain injury group (group T),and Xuebijing therapy group (group X).The model of traumatic brain injury was produced by improved Feeney' s traumatic brain injury model.Group S drill bone window and not crack down on brain tissue.The rabbits were sacrificed for injury brain tissue samples at 24 h after molding success in every groups.Brain cell apoptosis was evaluated by TUNEL staining.The cerebral expression of Bcl-2 and Bax was analyzed by Western blot.Results Compared with group S,the rates of apoptosis were increased and the protein expression ratios of Bcl-2/Bax were decreased in group T,and group X (P ＜0.01).Compared with group T,the rates of apoptosis were decreased and the protein expression ratios of Bcl-2/Bax were increased in group X (P ＜ 0.01).Conclusions Xuebijing can reduce the apoptosis of cerebral cells induced by traumatic brain injury to a certain extent,and protect the cerebrum,the signal of Bcl-2/Bax change to the way of anti-apoptosis is involved in the mechanism.
