CAJ | 학술논문

目的观察聚腺苷二磷酸核糖聚合酶(PARP)及核因子kappa B(NF-κB)在重症急性胰腺炎(SAP)大鼠腺垂体中的表达,并探讨其在SAP大鼠腺垂体损伤中的作用.方法雄性Wistar大鼠40只,随机(随机数字法)分为5组(n=8):假手术组(SO组)及重症急性胰腺炎组(SAP组)1、3、6、12 h组.胆胰管逆行注射5％牛磺胆酸钠制备重症急性胰腺炎模型.测定腹水量,血清淀粉酶、脂肪酶水平.光镜观察胰腺及垂体病理改变,电镜观察垂体超微结构改变.免疫组化观察垂体PARP及NF-κB的表达.结果 SAP组血清淀粉酶、脂肪酶水平及胰腺病理学评分逐渐增加,均较SO组明显升高(P＜0.05).光镜下可见SAP大鼠腺垂体细胞水肿及坏死；电镜下见SAP大鼠腺垂体出现核固缩、内质网及线粒体肿胀.PARP和NF-κB在SAP大鼠腺垂体中随时间点延长,其表达逐渐增强,均高于SO组表达.结论在重症急性胰腺炎时,大鼠腺垂体出现病理结构及超微结构损伤,PARP及NF-κB通路的变化可能参与其中.
목적 관찰취선감이린산핵당취합매(PARP)급핵인자kappa B(NF-κB)재중증급성이선염(SAP)대서선수체중적표체,병탐토기재SAP대서선수체손상중적작용.방법 웅성Wistar대서40지,수궤(수궤수자법)분위5조(n=8):가수술조(SO조)급중증급성이선염조(SAP조)1、3、6、12 h조.담이관역행주사5％우광담산납제비중증급성이선염모형.측정복수량,혈청정분매、지방매수평.광경관찰이선급수체병리개변,전경관찰수체초미결구개변.면역조화관찰수체PARP급NF-κB적표체.결과 SAP조혈청정분매、지방매수평급이선병이학평분축점증가,균교SO조명현승고(P＜0.05).광경하가견SAP대서선수체세포수종급배사；전경하견SAP대서선수체출현핵고축、내질망급선립체종창.PARP화NF-κB재SAP대서선수체중수시간점연장,기표체축점증강,균고우SO조표체.결론 재중증급성이선염시,대서선수체출현병리결구급초미결구손상,PARP급NF-κB통로적변화가능삼여기중.
Objective To investigate the changes of poly-ADP-ribose polymerase (PARP) and NF kappa B (NF-κB) in adenohypophysis in rat model of severe acute pancreatitis (SAP),and their role in the mechanism of adenohypophysis injury in SAP.Methods Forty Wistar rats were randomly (random number) divided into 5 groups:the sham operation group (SO group,n =8),SAP 1 h,3 h,6 h and 12 h groups (n =8 in each group).SAP model was induced by retrograde injection of 5％ sodium taurocholate into the biliopancreatic duct.Serum levels of amylase,lipase and ascites were measured.After sacrifice of experiment rats,pancreas and adenohypophysis tissues were taken for pathological examination under light microscope.Adenohypophysis cells were observed under electronic microscopy as well.PARP and NF-κB expressions in adenohypophysis cell was studied by using immunohistochemisty assay.Results After modelling,serum levels of amylase,lipase and ascites in SAP group increased gradually,which were higher than those in SO group (P ＜ 0.05).Adenohypophysis cell swelling and partial necrosis were observed under light microscope.As the time prolonged,their nuclei became dark and pyknotic more and more,and the endoplasmic reticulum and mitochondrial swelling in adenohypophysis cells were observed under electronic microscopy.The expressions of PARP and NF-κB in SAP group increased gradually,which were higher than those in SO group.Conclusions Significant pathological and ultrastructural injuries were observed in adenohypophysis cells in severe acute pancreatitis.These changes might correlate with PARP and NF-κB signaling pathway.