CAJ | 학술논문

目的观察百草枯(PQ)致急性肺损伤模型中早期生长反应因子-1 mRNA (Egr-1mRNA)、白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的变化以及姜黄素的干预作用.方法成年雄性SD大鼠随机(随机数字法)分成对照组、中毒组、干预组,各组再按3h、6h、24 h、3d、7d时间点分5个亚组.中毒组腹腔注射PQ 15 mg/kg,干预组腹腔注射姜黄素200 mg/kg 15 min后腹腔注射PQ 15 mg/kg,对照组腹腔注射生理盐水1 mL.荧光实时定量PCR法测定各组大鼠肺组织中Egr-1 mRNA表达量,ELISA法测定各组支气管肺泡灌洗液(BALF)中白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平,光镜下观察各组肺组织病理变化.实验数据统计采用单因素方差分析.组间比较用Bonferroni法.结果与对照组比较,中毒组和干预组肺组织Egr-1 mRNA表达量和BALF中TNF-α水平、中毒组BALF中IL-1β水平在染毒后6h、24 h、3d、7d均明显升高(P＜0.05或P＜0.01),干预组BALF中IL-1β水平在染毒后24 h明显升高(P＜0.05);与中毒组比较,干预组肺组织Egr-1 mRNA表达量、BALF中IL-1β和TNF-α水平在染毒后24 h、3d、7d均明显下降(P＜0.05或P＜0.01);中毒组病理改变严重,干预组病理改变较中毒组减轻.结论肺组织Egr-1 mRNA表达上调,TNF-α、IL-1β释放增加,参与启动炎症反应,可能是PQ中毒急性肺损伤的机制之一,姜黄素可能通过此信号通路减轻肺损伤的程度.
목적 관찰백초고(PQ)치급성폐손상모형중조기생장반응인자-1 mRNA (Egr-1mRNA)、백개소-1β(IL-1β)、종류배사인자-α(TNF-α)적변화이급강황소적간예작용.방법 성년웅성SD대서수궤(수궤수자법)분성대조조、중독조、간예조,각조재안3h、6h、24 h、3d、7d시간점분5개아조.중독조복강주사PQ 15 mg/kg,간예조복강주사강황소200 mg/kg 15 min후복강주사PQ 15 mg/kg,대조조복강주사생리염수1 mL.형광실시정량PCR법측정각조대서폐조직중Egr-1 mRNA표체량,ELISA법측정각조지기관폐포관세액(BALF)중백개소-1β(IL-1β)、종류배사인자-α(TNF-α)수평,광경하관찰각조폐조직병리변화.실험수거통계채용단인소방차분석.조간비교용Bonferroni법.결과 여대조조비교,중독조화간예조폐조직Egr-1 mRNA표체량화BALF중TNF-α수평、중독조BALF중IL-1β수평재염독후6h、24 h、3d、7d균명현승고(P＜0.05혹P＜0.01),간예조BALF중IL-1β수평재염독후24 h명현승고(P＜0.05);여중독조비교,간예조폐조직Egr-1 mRNA표체량、BALF중IL-1β화TNF-α수평재염독후24 h、3d、7d균명현하강(P＜0.05혹P＜0.01);중독조병리개변엄중,간예조병리개변교중독조감경.결론 폐조직Egr-1 mRNA표체상조,TNF-α、IL-1β석방증가,삼여계동염증반응,가능시PQ중독급성폐손상적궤제지일,강황소가능통과차신호통로감경폐손상적정도.
Objective To observe the changes of early growth response factor-1 mRNA (Egr-1 mRNA),interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in rat with paraquat (PQ)-induced acute lung injury,and the potential protective effect of curcumin on this injury.Methods Adult male Sprague-Dauley (SD) rats were randomly (random number) divided into control,PQ-poisoning and curcumin-intervention groups.At 3 h,6 h,24 h,3 d,7 d after treatment,each group respectively further divided into 5 subgroups.Rats in PQ-poisoning group were intraperitoneally (IP) administered with PQ 15 mg/kg,and rats in cureumin-intervention group were administered with PQ 15 mg/kg IP at 15 min after curcumin 200 mg/kg IP.Rats in control group were given normal saline (NS) 1 mL IP instead.The expression of Egr-1 mRNA in lungs tissue were measured with Fluorescence real-time quantitative PCR device.The levels of IL-1β and TNF-α in bronchoalveolar lavage fluid (BALF) were measured with enzyme-hnked immunosorbent assay (ELISA).Pathological changes in lung were observed under light microscope.Experimental data were treated with single-factor analysis of variance and compared between groups with Bonferroni method.Results Compared with the control group,Egr-1 mRNA expression in lungs tissue and TNF-α level in BALF in PQ-poisoning group and curcumin-intervention group,IL-1β level in BALF in PQ-poisoning group increased significantly at 6 h,24 h,3 d,7 d after PQ exposure (P ＜ 0.05 or P ＜ 0.01),and IL-1β level in BALF increased significantly at 24 h after PQ exposure in curcuminintervention group (P ＜ 0.05).Compared with the PQ-poisoning group,Egr-1 mRNA expression in lungs tissue,TNF-α and IL-1 β level in BALF decreased significantly at 24 h,3 d,7 d after PQ exposure in curcumin-intervention group (P ＜ 0.05 or P ＜ 0.01).Pathological changes of lung tissue were more serious in PQ-poisoning group than those in curcumin-intervention group.Conclusions The up-regulated expression of Egr-1 mRNA increased releasing ofIL-1β and TNF-α,and initiating inflammation may be one of the mechanism of PQ-induced acute lung injury in rats.Curcumin may have beneficial protective effects on lung in this above described pathway of signaling.