中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2014年
8期
852-856
,共5页
多器官功能障碍综合征%肾损伤%水通道蛋白%钠-钾-三磷酸腺苷酶%内毒素%炎症%脂多糖%能量代谢
多器官功能障礙綜閤徵%腎損傷%水通道蛋白%鈉-鉀-三燐痠腺苷酶%內毒素%炎癥%脂多糖%能量代謝
다기관공능장애종합정%신손상%수통도단백%납-갑-삼린산선감매%내독소%염증%지다당%능량대사
Multiple organ dysfunction syndrome%Kidney injury%Water channel protein%Na+-K+-ATPase%Endotoxin%Inflammation%Lipopolysaccharide%Energy metabolism
目的 探讨水通道蛋白2的表达及钠-钾-三磷酸腺苷酶在多器官功能障碍肾损伤发病机制中的作用,研究大鼠多器官功能障碍肾损伤与水通道蛋白2及钠-钾-三磷酸腺苷酶的表达.方法 选取健康大鼠72只,随机(随机数字法)分为对照组24只,脂多糖组48只,采用大鼠腹腔注射脂多糖5 mg/kg造成内毒素致多器官功能障碍动物模型,对照组仅作假手术处理.造模成功后6h、12h、24 h、2d、3d、5d分别处死动物,留取血液、尿量进行生化检测,并应用免疫组化法和RT-PCR技术检测肾组织内水通道蛋白2 mRNA及蛋白的表达水平.应用试剂盒测定钠-钾-三磷酸腺苷酶的含量及活性.结果 造模成功后致伤组大鼠尿量迅速减少,48 h后尿量增多.尿素氮、肌酐逐渐增高,48 h达高峰,此后逐渐下降.水通道蛋白2 mRNA及蛋白表达迅速减少,48 h降至最低,此后逐渐增多.钠-钾-三磷酸腺苷酶含量差异无统计学意义,但其活性在造模成功后明显降低,此后逐渐增高,但仍低于对照组.结论 大鼠多器官功能障碍综合征肾损伤模型中,水通道蛋白2是肾脏重吸收功能恢复的结构基础,钠-钾-三磷酸腺苷酶则直接参与或间接反映肾脏的能量代谢状态,只有在水通道蛋白2及能量代谢恢复后,大鼠的肾脏功能才能逐渐好转.
目的 探討水通道蛋白2的錶達及鈉-鉀-三燐痠腺苷酶在多器官功能障礙腎損傷髮病機製中的作用,研究大鼠多器官功能障礙腎損傷與水通道蛋白2及鈉-鉀-三燐痠腺苷酶的錶達.方法 選取健康大鼠72隻,隨機(隨機數字法)分為對照組24隻,脂多糖組48隻,採用大鼠腹腔註射脂多糖5 mg/kg造成內毒素緻多器官功能障礙動物模型,對照組僅作假手術處理.造模成功後6h、12h、24 h、2d、3d、5d分彆處死動物,留取血液、尿量進行生化檢測,併應用免疫組化法和RT-PCR技術檢測腎組織內水通道蛋白2 mRNA及蛋白的錶達水平.應用試劑盒測定鈉-鉀-三燐痠腺苷酶的含量及活性.結果 造模成功後緻傷組大鼠尿量迅速減少,48 h後尿量增多.尿素氮、肌酐逐漸增高,48 h達高峰,此後逐漸下降.水通道蛋白2 mRNA及蛋白錶達迅速減少,48 h降至最低,此後逐漸增多.鈉-鉀-三燐痠腺苷酶含量差異無統計學意義,但其活性在造模成功後明顯降低,此後逐漸增高,但仍低于對照組.結論 大鼠多器官功能障礙綜閤徵腎損傷模型中,水通道蛋白2是腎髒重吸收功能恢複的結構基礎,鈉-鉀-三燐痠腺苷酶則直接參與或間接反映腎髒的能量代謝狀態,隻有在水通道蛋白2及能量代謝恢複後,大鼠的腎髒功能纔能逐漸好轉.
목적 탐토수통도단백2적표체급납-갑-삼린산선감매재다기관공능장애신손상발병궤제중적작용,연구대서다기관공능장애신손상여수통도단백2급납-갑-삼린산선감매적표체.방법 선취건강대서72지,수궤(수궤수자법)분위대조조24지,지다당조48지,채용대서복강주사지다당5 mg/kg조성내독소치다기관공능장애동물모형,대조조부작가수술처리.조모성공후6h、12h、24 h、2d、3d、5d분별처사동물,류취혈액、뇨량진행생화검측,병응용면역조화법화RT-PCR기술검측신조직내수통도단백2 mRNA급단백적표체수평.응용시제합측정납-갑-삼린산선감매적함량급활성.결과 조모성공후치상조대서뇨량신속감소,48 h후뇨량증다.뇨소담、기항축점증고,48 h체고봉,차후축점하강.수통도단백2 mRNA급단백표체신속감소,48 h강지최저,차후축점증다.납-갑-삼린산선감매함량차이무통계학의의,단기활성재조모성공후명현강저,차후축점증고,단잉저우대조조.결론 대서다기관공능장애종합정신손상모형중,수통도단백2시신장중흡수공능회복적결구기출,납-갑-삼린산선감매칙직접삼여혹간접반영신장적능량대사상태,지유재수통도단백2급능량대사회복후,대서적신장공능재능축점호전.
Objective To investigate the role of AQP2 and Na +-K +-ATPase in the pathogenesis of kidney injury with multiple organ dysfunction syndrome,and try to find the express characteristics of them.Methods A total of 72 healthy rats were randomly (random number) divided into two groups:control group (n =24) and Lipopolysaccharide group (n =48).The Lipopolysaccharide group rats were injected with 5 mg/kg lipopolysaccharide at the beginning while the control group was 0.9％ sodium chloride.After the model was succeeded,the rats were put to death at 6 h,12 h,24 h,2 days,3 days and 5 days equally.The urine and blood were collected.Blood were used biochemical tests to check.kidney AQP2 protein and mRNA expression level in the organization were applied the immune organized and RT-PCR technique to detect.Applied kit for determining the content and activity of sodium/potassium-atpase.Results The volume of urine in LPS group decrease quickly at 12 h and 24 h,but increased after 2 days.Urea nitrogen and creatinine increased gradually,and peaked at 48 h,after then gradually decline.AQP2 mRNA and protein expression decreased,and minimize at 48 h.The content of Na+-K+-ATP ase has no obvious difference,but the activity significantly decreased at the beginning,then increased gradually,but it was still lower than the control group.Conclusions In renal injury rats model with multiple organ dysfunction syndrome,AQP2 is the structure of renal reabsorption function,while Na +-K +-ATPase directly involved in or indirectly reflected the state of kidney energy metabolism.Recovery of AQP2 protein and energy metabolism,before the rat kidney function improved.
