CAJ | 학술논문

目的探索内源性硫化氢对于油酸诱导的急性肺损伤大鼠肺泡上皮细胞内质网应激的调节作用.方法雄性Sprague Dawley大鼠随机(随机数字法)被分入正常对照组、油酸组、油酸+硫氢化钠组及硫氢化钠对照组.每个组进一步分为2、4和6h3个时间点.对肺组织进行肺组织损伤半定量评分,检测肺组织湿、干质量比和匀浆硫化氢含量.通过免疫组化染色和蛋白印迹法检测内质网应激的标志蛋白(GRP78和elF2α)的表达.结果肺损伤大鼠肺组织损伤半定量评分及湿、干质量比明显升高,而肺组织硫化氢含量,肺泡上皮细胞GRP78及eIF2α的表达明显降低.在硫氢化钠预处理的大鼠中,肺损伤半定量评分及肺组织湿、干质量比明显降低,而肺组织硫化氢含量,肺泡上皮细胞GRP78及eIF2α的表达明显升高.结论内源性硫化氢可以通过促进肺泡上皮细胞内质网应激反应进而在肺损伤过程中起到保护作用.
목적 탐색내원성류화경대우유산유도적급성폐손상대서폐포상피세포내질망응격적조절작용.방법 웅성Sprague Dawley대서수궤(수궤수자법)피분입정상대조조、유산조、유산+류경화납조급류경화납대조조.매개조진일보분위2、4화6h3개시간점.대폐조직진행폐조직손상반정량평분,검측폐조직습、간질량비화균장류화경함량.통과면역조화염색화단백인적법검측내질망응격적표지단백(GRP78화elF2α)적표체.결과 폐손상대서폐조직손상반정량평분급습、간질량비명현승고,이폐조직류화경함량,폐포상피세포GRP78급eIF2α적표체명현강저.재류경화납예처리적대서중,폐손상반정량평분급폐조직습、간질량비명현강저,이폐조직류화경함량,폐포상피세포GRP78급eIF2α적표체명현승고.결론 내원성류화경가이통과촉진폐포상피세포내질망응격반응진이재폐손상과정중기도보호작용.
Objective To study the regulatory effect of hydrogen sulfide (H2S) on endoplasmic reticulum stress in alveolar epithelial cells of rats with acute lung injury (ALI) induced by oleic acid (OA).Methods Seventy-two male Sprague Dawley (SD) rats were equally divided into control group (C group),oleic acid-induced ALI group (OA group),oleic acid-induced ALI with sodium hydrosulfide (NaHS) pretreatment group (OA + NaHS group) and sodium hydrosulfide treatment group (NaHS group).The model of acute lung injury was made by oleic acid intravenous injection in dose of 0.1 mL/kg.NaHS was injected intra-abdominally in dose of 1 ml/kg with concentration of 56 μmol/L 30 min before administration of oleic acid for pretreatment.In control groups,saline was used instead of oleic acid and NaHS in the equivalent volume.Six rats of each group were sacrificed at 2 h,4 h and 6 hours separately after modeling for observing the acute injury of lung tissue.Index of quantitative assessment of histological lung injury (IQA),wet/dry weight ratio (W/D) and H2S level of lung tissues were measured.The endoplasmic reticulum stress markers included glucose-regulated protein 78 (GRP78) and α-subunit of eukaryotic translation initiation factor-2 (elF2α) in lung tissues were measured by immunohistochemical staining and Western blot.Results At the three observation intervals,the IQA score and W/D ratio of lung tissues significantly increased in rats-after OA injected,but significantly decreased in other rats receiving both OA and NaHS.At the three intervals,the level of H2S in lung tissue significantly decreased in rats after OA injected,but significantly increased in other rats receiving both OA and NaHS.GRP78 and elF2α decreased in rats after OA injected,but increased in other rats receiving both OA and NaHS especially at 4 h and 6 h after modeling.Conclusions The findings suggested that H2S could promote the response to alveolar epithelial cell endoplasmic reticulum stress in rats with ALI resulting in attenuating the damage of lung tissue caused by oleic acid.