中华口腔正畸学杂志
中華口腔正畸學雜誌
중화구강정기학잡지
CHINESE JOURNAL OF ORTHODONTICS
2013年
3期
150-155
,共6页
气体信号分子%硫化氢(H2S)%一氧化氮(NO)%正畸牙移动%牙周组织改建
氣體信號分子%硫化氫(H2S)%一氧化氮(NO)%正畸牙移動%牙週組織改建
기체신호분자%류화경(H2S)%일양화담(NO)%정기아이동%아주조직개건
gasotransmitter%hydrogen sulfide%nitricoxide%orthodontic tooth movement%periodontal tissue remodeling
目的 观察硫化氢(hydrogen sulfide,H2S)对大鼠正畸牙牙周组织改建的影响,并与一氧化氮(nitric oxide,NO)对比,初步探讨其在改建过程中的作用及机制.方法 利用45只雄性Sprague-Dawley(SD)大鼠建立正畸牙移动模型,动物随机分为三组:阴性对照组(A组)局部注射无菌磷酸缓冲盐溶液(phosphate-buffered saline,PBS);实验组(B组)局部注射40 mg/ml DL-炔丙基甘氨酸(DL-propargylglycine,PPG);阳性对照组(C组)注射相同浓度NG-硝基精氨酸甲酯(NG-Nitro-L-arginineMethylEster,L-NAME).于加力第7,14,21天分批处死大鼠,制作切片.应用抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色计数破骨细胞数,链霉亲和素-生物素复合物(strept avidin-biotin complex,SABC)染色检测胱硫醚-γ-裂解酶(eystathionine γ-lyase,CSE)及诱导型一氧化氮合酶(inducible nitric oxide synthases,iNOS)阳性表达的平均光密度值.结果 加力第7天,B组破骨细胞数明显少于其余两组(P＜0.01),CSE表达量在C组中最高.加力14,21天B组破骨细胞数呈先增加后减少趋势,A、C两组则呈递减趋势;CSE和iNOS表达量均逐渐减少,但21天时CSE基本呈阴性表达,而iNOS表达量仍较高(P＜0.01).结论 应用40 mg/ml PPG可减少正畸牙早期H2S的生成,抑制破骨细胞募集;在正畸牙移动牙周组织改建过程中,NO/iNOS与H2 S/CSE体系间可能存在负性调节作用.
目的 觀察硫化氫(hydrogen sulfide,H2S)對大鼠正畸牙牙週組織改建的影響,併與一氧化氮(nitric oxide,NO)對比,初步探討其在改建過程中的作用及機製.方法 利用45隻雄性Sprague-Dawley(SD)大鼠建立正畸牙移動模型,動物隨機分為三組:陰性對照組(A組)跼部註射無菌燐痠緩遲鹽溶液(phosphate-buffered saline,PBS);實驗組(B組)跼部註射40 mg/ml DL-炔丙基甘氨痠(DL-propargylglycine,PPG);暘性對照組(C組)註射相同濃度NG-硝基精氨痠甲酯(NG-Nitro-L-arginineMethylEster,L-NAME).于加力第7,14,21天分批處死大鼠,製作切片.應用抗酒石痠痠性燐痠酶(tartrate resistant acid phosphatase,TRAP)染色計數破骨細胞數,鏈黴親和素-生物素複閤物(strept avidin-biotin complex,SABC)染色檢測胱硫醚-γ-裂解酶(eystathionine γ-lyase,CSE)及誘導型一氧化氮閤酶(inducible nitric oxide synthases,iNOS)暘性錶達的平均光密度值.結果 加力第7天,B組破骨細胞數明顯少于其餘兩組(P＜0.01),CSE錶達量在C組中最高.加力14,21天B組破骨細胞數呈先增加後減少趨勢,A、C兩組則呈遞減趨勢;CSE和iNOS錶達量均逐漸減少,但21天時CSE基本呈陰性錶達,而iNOS錶達量仍較高(P＜0.01).結論 應用40 mg/ml PPG可減少正畸牙早期H2S的生成,抑製破骨細胞募集;在正畸牙移動牙週組織改建過程中,NO/iNOS與H2 S/CSE體繫間可能存在負性調節作用.
목적 관찰류화경(hydrogen sulfide,H2S)대대서정기아아주조직개건적영향,병여일양화담(nitric oxide,NO)대비,초보탐토기재개건과정중적작용급궤제.방법 이용45지웅성Sprague-Dawley(SD)대서건립정기아이동모형,동물수궤분위삼조:음성대조조(A조)국부주사무균린산완충염용액(phosphate-buffered saline,PBS);실험조(B조)국부주사40 mg/ml DL-결병기감안산(DL-propargylglycine,PPG);양성대조조(C조)주사상동농도NG-초기정안산갑지(NG-Nitro-L-arginineMethylEster,L-NAME).우가력제7,14,21천분비처사대서,제작절편.응용항주석산산성린산매(tartrate resistant acid phosphatase,TRAP)염색계수파골세포수,련매친화소-생물소복합물(strept avidin-biotin complex,SABC)염색검측광류미-γ-렬해매(eystathionine γ-lyase,CSE)급유도형일양화담합매(inducible nitric oxide synthases,iNOS)양성표체적평균광밀도치.결과 가력제7천,B조파골세포수명현소우기여량조(P＜0.01),CSE표체량재C조중최고.가력14,21천B조파골세포수정선증가후감소추세,A、C량조칙정체감추세;CSE화iNOS표체량균축점감소,단21천시CSE기본정음성표체,이iNOS표체량잉교고(P＜0.01).결론 응용40 mg/ml PPG가감소정기아조기H2S적생성,억제파골세포모집;재정기아이동아주조직개건과정중,NO/iNOS여H2 S/CSE체계간가능존재부성조절작용.
Objective To assess the effects of H2S on periodontal tissue remodeling and possible interaction between H2S and NO in rat model of orthodontic tooth movement (OTM).Methods The OTM models were established in three groups of 45 male Sprague-Dawley rats.The rats in the negativecontrol group (Group A) received injection of sterile phosphate-buffered saline (PBS),and those in the treatment group (Group B) and positive control group (Group C) respectively received 40 mg/ml DL-propargylglycine (PPG) and NG-Nitro-L-arginine Methyl Ester (L-NAME).Histochemistry and immunohistochemistry were performed respectively to exam the number of tartrate-resistant acid phosphatase(TRAP) positive osteoclast and expression of cystathionine γ-lyase (CSE) and inducible nitric oxide sythase (iNOS) in the tissue sections containing intact roots and periodontal tissues of the first molar on day 7,14 and 21 after appliance insertion.Results Group B had lest osteoclasts on day 7,compared to the other two groups(P＜0.01) while Group C showed the most expression of CSE.Increasing number of osteoclasts were observed in the first 14 days but followed with a decrease on day 21 in Group B.Osteoclast number decreased in Group A and C during the course.The expressions of both enzymes were down-regulated gradually.On day 21,CSE expression was negative whereas iNOS remained relative high expression(P＜0.01).Conclusions Application of 40 mg/ml PPG can reduce the generation of the endogenous H2S,and prevent osteoclasts from recruiting in the early stage of OTM.There may be down-regulation effect between NO/iNOS and H2 S/CSE during orthodontic periodontal tissue remodeling.
