CAJ | 학술논문

目的研究不同牙周状态正畸力作用下IL-23在牙周组织的表达变化,为牙周病正畸治疗提供参考.方法建立大鼠牙周炎静止期、牙周炎活动期模型,以50克力移动牙周正常组、牙周炎静止期组、牙周炎活动期组的上颌磨牙.分别于牙齿移动的第3和7天处死大鼠.采用免疫组化和实时荧光定量PCR定性定量分析各组IL-23在牙周组织的表达.结果正常牙周移动组IL-23mRNA的表达较正常对照组差异无显著性(P＞0.05).静止期牙齿移动组IL-23mRNA在牙周组织的表达较正常牙周移动组增高,差异有显著性(P＜0.05);较活动期移动组显著减小(P＜0.01).牙周炎活动期牙齿移动组IL-23mRNA的表达较其他各组均高,且差异有高度显著性(P＜0.01).结论 IL-23参与调节牙周炎正畸牙齿移动.牙周炎静止期正畸治疗不会导致IL-23的过度高表达,但要密切控制牙周易感因素.
목적 연구불동아주상태정기력작용하IL-23재아주조직적표체변화,위아주병정기치료제공삼고.방법 건립대서아주염정지기、아주염활동기모형,이50극력이동아주정상조、아주염정지기조、아주염활동기조적상합마아.분별우아치이동적제3화7천처사대서.채용면역조화화실시형광정량PCR정성정량분석각조IL-23재아주조직적표체.결과 정상아주이동조IL-23mRNA적표체교정상대조조차이무현저성(P＞0.05).정지기아치이동조IL-23mRNA재아주조직적표체교정상아주이동조증고,차이유현저성(P＜0.05);교활동기이동조현저감소(P＜0.01).아주염활동기아치이동조IL-23mRNA적표체교기타각조균고,차차이유고도현저성(P＜0.01).결론 IL-23삼여조절아주염정기아치이동.아주염정지기정기치료불회도치IL-23적과도고표체,단요밀절공제아주역감인소.
Objective To detect IL-23 expression in periodontal ligament induced by orthodontic force under different periodontal conditions and to guide clinic orthodontic treatment of patients with periodontitis.Methods Active periodontitis and non-active periodontitis models were built.Forty two male SD rats were randomly divided into three groups:healthyperiodontium movement,active periodontitis movement and non-active periodontitis movement.50g of mesial force was applied to move unilateral maxillary first molars of all three groups.At day 3 and 7,rats weresacrificed.By means of immunohistochemistry and RT-qPCR,IL-23 mRNA expressionin periodontal ligament were analyzed.Results The difference of IL-23mRNA expression between normal negative control group and healthyperiodontium movement group was not significant (P＞0.05).Compared with healthyperiodontium group,the expression of IL-23 mRNA in non-active periodontitis group had significantly increased (P＜0.05).However,it was less than that of positive periodontitis control (P＜0.01).Active periodontitis movement group presented a remarkable increase in IL-23mRNA expression than normal negative control,positive periodontitis control,healthyperiodontium and non-active periodontitis groups (P＜0.01).Conclusions IL-23 plays a role in regulating orthodontictooth movement with periodontitis.Orthodontic force causes limited increase of IL-23 mRNA expression in non-active periodontal tissues.During orthodontic treatment,careful monitoring of the periodontal predisposing factors is necessary.