CAJ | 학술논문

目的建立生长发育期大鼠的间歇性双侧完全鼻阻塞的动物模型.方法 30只3周龄SD大鼠,分为3组,A组:对照组,B组:单侧鼻阻塞组,C组:双侧鼻阻塞组(张口呼吸组),每组各10只.用特制的阻塞器阻塞大鼠鼻腔,阻塞的时间为8:00am至4:00pm,4周后处死30只大鼠.结果 A组、B组大鼠以鼻呼吸为主;C组表现为张口呼吸.A、B、C三组的呼吸频率分别为(96.8±5.91)次/min、(92.8±2.98)次/min和(48.6±4.38)次/min,C组的呼吸频率较A组与B组明显减慢(P＜0.001).建模4周时,A、B、C三组的体重分别为(276.60±20.17)g、(236.80±19.46)g和(127.33±24.8)g,C组的体重较A组和B组明显的降低(P＜0.001).结论本研究通过间歇性的双侧完全阻塞大鼠的外鼻初步建立了一个存活率高、重复性较好与人类张口呼吸较相似的大鼠的间歇性张口呼吸模型.
목적 건립생장발육기대서적간헐성쌍측완전비조새적동물모형.방법 30지3주령SD대서,분위3조,A조:대조조,B조:단측비조새조,C조:쌍측비조새조(장구호흡조),매조각10지.용특제적조새기조새대서비강,조새적시간위8:00am지4:00pm,4주후처사30지대서.결과 A조、B조대서이비호흡위주;C조표현위장구호흡.A、B、C삼조적호흡빈솔분별위(96.8±5.91)차/min、(92.8±2.98)차/min화(48.6±4.38)차/min,C조적호흡빈솔교A조여B조명현감만(P＜0.001).건모4주시,A、B、C삼조적체중분별위(276.60±20.17)g、(236.80±19.46)g화(127.33±24.8)g,C조적체중교A조화B조명현적강저(P＜0.001).결론 본연구통과간헐성적쌍측완전조새대서적외비초보건립료일개존활솔고、중복성교호여인류장구호흡교상사적대서적간헐성장구호흡모형.
Objective To build up a steady and credible animal model of bilateral intermittent nasal obstruction in young rats.Methods Thirty 3-week-old SD rats were employed and divided into group A(control),group B(left nostril occluded),and group C(both nostrils occluded by nose plugs).The occlusion period was between 8 a.m.and 4 p.m.every day.Four weeks later all rats were sacrificed.Results Rats in group A and B remained nose breathing,but group C developed mouth breathing.The mean respiratory rate of rats in group A,B and C were (96.8±5.91),(92.8±2.98) and (48.6 ± 4.38) respectively.Group C was significantly slower compared with group A and B (P＜0.001).The meanweight for group A,B and C were(276.60±20.17) g、(236.80±19.46) g and (127.33±24.8) g four weeks later,respectively.Group C reduced significantly compared with group A and B(P＜0.001).Conclusions The animal model of bilateral intermittent nasal obstruction in young rats established by occlusion with nose plugs was steady,credible and similar to oral breathing of human being.