中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2012年
6期
409-412
,共4页
鲁凯%姚壮凯%刘燕文%叶红玲%吕三云
魯凱%姚壯凱%劉燕文%葉紅玲%呂三雲
로개%요장개%류연문%협홍령%려삼운
乳腺癌%Survivin%RAGE%HMGB1%淋巴结转移%Real-time PCR%荧光原位杂交技术(FISH)
乳腺癌%Survivin%RAGE%HMGB1%淋巴結轉移%Real-time PCR%熒光原位雜交技術(FISH)
유선암%Survivin%RAGE%HMGB1%림파결전이%Real-time PCR%형광원위잡교기술(FISH)
Breast cancer%Survivin%RAGE%HMGB1%Lymph node metastasis%Real-time PCR%FISH
目的 探讨乳腺癌组织中Survivin、RAGE和HMGB1基因表达及其临床意义.方法 对50例早期(Ⅰ+Ⅱ期)、50例晚期(Ⅲ+Ⅳ期)乳腺癌及100例对照组蜡块标本,运用Real-time PCR和荧光原位杂交技术(FISH)技术检测Survivin、RAGE和HMGB1基因表达.并分析各基因表达与乳腺癌组织的分化程度、浸润深度、淋巴结转移、TNM分期之间的关系.结果 Survivin、RAGE和HMGB1基因表达荧光实时定量PCR法上调分别为62％、73％、79％,FISH法基因扩增阳性分别为78％、69％、72％.乳腺癌TNM分期、淋巴结转移与基因高表达有密切关系,Survivin基因表达与RAGE、HMGB1表达呈正相关.结论 Survivin、RAGE和HMGB1基因表达对乳腺癌早期诊断和预后分析有重要指导意义.
目的 探討乳腺癌組織中Survivin、RAGE和HMGB1基因錶達及其臨床意義.方法 對50例早期(Ⅰ+Ⅱ期)、50例晚期(Ⅲ+Ⅳ期)乳腺癌及100例對照組蠟塊標本,運用Real-time PCR和熒光原位雜交技術(FISH)技術檢測Survivin、RAGE和HMGB1基因錶達.併分析各基因錶達與乳腺癌組織的分化程度、浸潤深度、淋巴結轉移、TNM分期之間的關繫.結果 Survivin、RAGE和HMGB1基因錶達熒光實時定量PCR法上調分彆為62％、73％、79％,FISH法基因擴增暘性分彆為78％、69％、72％.乳腺癌TNM分期、淋巴結轉移與基因高錶達有密切關繫,Survivin基因錶達與RAGE、HMGB1錶達呈正相關.結論 Survivin、RAGE和HMGB1基因錶達對乳腺癌早期診斷和預後分析有重要指導意義.
목적 탐토유선암조직중Survivin、RAGE화HMGB1기인표체급기림상의의.방법 대50례조기(Ⅰ+Ⅱ기)、50례만기(Ⅲ+Ⅳ기)유선암급100례대조조사괴표본,운용Real-time PCR화형광원위잡교기술(FISH)기술검측Survivin、RAGE화HMGB1기인표체.병분석각기인표체여유선암조직적분화정도、침윤심도、림파결전이、TNM분기지간적관계.결과 Survivin、RAGE화HMGB1기인표체형광실시정량PCR법상조분별위62％、73％、79％,FISH법기인확증양성분별위78％、69％、72％.유선암TNM분기、림파결전이여기인고표체유밀절관계,Survivin기인표체여RAGE、HMGB1표체정정상관.결론 Survivin、RAGE화HMGB1기인표체대유선암조기진단화예후분석유중요지도의의.
Objective To investigate the expression of Survivin,receptor for advanced glycation endproduct(RAGE) and high mobility group protein B1 (HMGB1) gene in human breast cancer and their clinical significance.Methods The expression of Survivin,RAGE and HMGB1 gene was detected by Real-time PCR technology and fluorescence in situ hybridization (FISH) technology in the following tissue samples:50 early breast cancers,50 advanced breast cancers,and the corresponding adjacent normal mammary tissues.The relationship of their expression and several factors such as differentiation degree,invasion,lymph node metastasis and TNM stage of cancer was explored.Results The positive expression rate of Survivin,RAGE and HMGB1 gene was 62％,73％ and 79％ detected by Real-time PCR technology,78％,69％ and 72％ detected by FISH technology in breast cancer tissues.The overexpression of these genes was positively correlated with TNM stage and lymph node metastasis.The expression of Survivin gene was positively correlated with the expression of RAGE and HMGB1.Conclusion Overexpression of Survivin,RAGE and HMGB1 gene is of great significance in early diagnosis and prognosis of human breast cancer.
