中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2013年
1期
4-7
,共4页
鄢丹桂%张彬%邹霜梅%安常明%张宗敏%李正江%徐震纲%唐平章
鄢丹桂%張彬%鄒霜梅%安常明%張宗敏%李正江%徐震綱%唐平章
언단계%장빈%추상매%안상명%장종민%리정강%서진강%당평장
甲状腺乳头状癌%前哨淋巴结%微转移
甲狀腺乳頭狀癌%前哨淋巴結%微轉移
갑상선유두상암%전초림파결%미전이
Thyroid papillary carcinoma%Sentinel lymph node%Micrometastasis
目的 探讨检测甲状腺乳头状癌(papillary thyroid carcinoma,PTC)前哨淋巴结(sentinel lymph node,SLN)微转移的恰当方法.方法 前瞻性分析2007年8月至2010年9月51例中国医学科学院肿瘤医院头颈外科SLN研究小组收治的临床淋巴结阴性PTC,采用核素(淋巴闪烁显像+γ探针)、染料及联合应用染料与核素法定位SLN.对18例常规病理检查未发现转移的55枚SLN行多层间隔连续切片,分别行HE染色和细胞角蛋白(CK7)免疫组织化学染色,检测SLN微转移状况.结果 51例全部检出SLN,检出率100％(51/51).在常规病理切片检查SLN无转移的18例中,共发现5例微转移癌灶,SLN活检假阴性率由常规病理切片的15.4％降至2.6％.结论 连续切片和免疫组织化学染色是检测PTC的SLN微转移有效、可行的方法,能明显提高SLN活检的准确性.
目的 探討檢測甲狀腺乳頭狀癌(papillary thyroid carcinoma,PTC)前哨淋巴結(sentinel lymph node,SLN)微轉移的恰噹方法.方法 前瞻性分析2007年8月至2010年9月51例中國醫學科學院腫瘤醫院頭頸外科SLN研究小組收治的臨床淋巴結陰性PTC,採用覈素(淋巴閃爍顯像+γ探針)、染料及聯閤應用染料與覈素法定位SLN.對18例常規病理檢查未髮現轉移的55枚SLN行多層間隔連續切片,分彆行HE染色和細胞角蛋白(CK7)免疫組織化學染色,檢測SLN微轉移狀況.結果 51例全部檢齣SLN,檢齣率100％(51/51).在常規病理切片檢查SLN無轉移的18例中,共髮現5例微轉移癌竈,SLN活檢假陰性率由常規病理切片的15.4％降至2.6％.結論 連續切片和免疫組織化學染色是檢測PTC的SLN微轉移有效、可行的方法,能明顯提高SLN活檢的準確性.
목적 탐토검측갑상선유두상암(papillary thyroid carcinoma,PTC)전초림파결(sentinel lymph node,SLN)미전이적흡당방법.방법 전첨성분석2007년8월지2010년9월51례중국의학과학원종류의원두경외과SLN연구소조수치적림상림파결음성PTC,채용핵소(림파섬삭현상+γ탐침)、염료급연합응용염료여핵소법정위SLN.대18례상규병리검사미발현전이적55매SLN행다층간격련속절편,분별행HE염색화세포각단백(CK7)면역조직화학염색,검측SLN미전이상황.결과 51례전부검출SLN,검출솔100％(51/51).재상규병리절편검사SLN무전이적18례중,공발현5례미전이암조,SLN활검가음성솔유상규병리절편적15.4％강지2.6％.결론 련속절편화면역조직화학염색시검측PTC적SLN미전이유효、가행적방법,능명현제고SLN활검적준학성.
Objective To investigate the optimal methods of detecting micrometastasis of sentinel lymph node(SLN) in papillary thyroid carcinoma(PTC).Methods Data of 51 consecutive PTC patients without clinical evidence of cervical lymph node metastasis were analyzed.They were conducted with SLN localization with blue dye,technetium-labeled sulfur colloid or the combination of them from Aug.2007 to Sep.2010.55 SLNs from 18 cases were selected.No metastasis was found to these 55 SLNs by routine pathological section.The 55 SLNs were serially sectioned at a 50 μm interval and stained by both HE and immunohistochemistry for detecting micrometastasis.Results SLN was successfully identified in all the 51 cases,with SLN identification rate of 100％.Among the 18 cases without metastasis to SLN by routine pathological section,5 cases were found micrometastasis by step sectioning plus immunohistochemically stains.The false negative rate was reduced from 15.4％ by routine pathological section to 2.6％ by step sectioning plus immunohistochemically stains.Conclusion Cytokeratin immunohistochemistry on series sections is a reliable method in detecting SLN micrometastasis in PTC.
