中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2013年
3期
187-190
,共4页
杨远行%郗光霞%孙启政%辛欢欢
楊遠行%郗光霞%孫啟政%辛歡歡
양원행%치광하%손계정%신환환
胰高血糖素样肽-1%葡萄糖转运蛋白4%骨骼肌
胰高血糖素樣肽-1%葡萄糖轉運蛋白4%骨骼肌
이고혈당소양태-1%포도당전운단백4%골격기
Glucagon-like peptide-1%Glucose transporter 4%Skeletal muscle
目的 探讨胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)受体激动剂艾塞那肽对糖耐量减低(impaired glucose tolerance,IGT)大鼠骨骼肌葡萄糖转运蛋白4(glucose transporter4,GLUT4)表达的作用.方法 54只Wistar大鼠,按照完全随机化分组方法分为正常糖耐量组(NGT组,18只)和IGT组(36只),NGT组予常规饲料,IGT组予高糖高脂饲料.12周时IGT造模成功,成模后从IGT组随机抽取18只大鼠设为艾塞那肽干预组(Ex组),每日2次皮下注射艾塞那肽(5μg/kg);另外18只设为IGT对照组.NGT组及IGT对照组均给予等体积生理盐水皮下注射.于干预前和干预后4周分别测空腹血糖(fasting blood glucose,FBG)和餐后2h血糖(2 h BG);实时荧光定量PCR检测骨骼肌GLUT4mRNA的表达,免疫组织化学法检测骨骼肌GLUT4蛋白的表达.各组间两两比较采用单因素方差分析(ANOVA),LSD-t检验.结果 干预前,与NGT组比较,IGT对照组及Ex组2hBG增高,骨骼肌GLUT4mRNA表达降低,差异有统计学意义(均P ＜0.05).IGT对照组及Ex组骨骼肌细胞内棕黄色着色较少,NGT组着色广泛,着色颗粒较多.艾塞那肽干预4周后,与同期IGT对照组及干预前Ex组比较,Ex组2 hBG下降,骨骼肌GLUT4 mRNA表达增高,差异均有统计学意义(P＜0.05).干预后Ex组较同期IGT对照组及干预前Ex组骨骼肌细胞内棕黄色着色增多,主要表达在细胞浆中.结论 艾塞那肽可上调骨骼肌GLUT4的表达,促进骨骼肌葡萄糖的摄取,降低餐后血糖.
目的 探討胰高血糖素樣肽-1(glucagon-like peptide-1,GLP-1)受體激動劑艾塞那肽對糖耐量減低(impaired glucose tolerance,IGT)大鼠骨骼肌葡萄糖轉運蛋白4(glucose transporter4,GLUT4)錶達的作用.方法 54隻Wistar大鼠,按照完全隨機化分組方法分為正常糖耐量組(NGT組,18隻)和IGT組(36隻),NGT組予常規飼料,IGT組予高糖高脂飼料.12週時IGT造模成功,成模後從IGT組隨機抽取18隻大鼠設為艾塞那肽榦預組(Ex組),每日2次皮下註射艾塞那肽(5μg/kg);另外18隻設為IGT對照組.NGT組及IGT對照組均給予等體積生理鹽水皮下註射.于榦預前和榦預後4週分彆測空腹血糖(fasting blood glucose,FBG)和餐後2h血糖(2 h BG);實時熒光定量PCR檢測骨骼肌GLUT4mRNA的錶達,免疫組織化學法檢測骨骼肌GLUT4蛋白的錶達.各組間兩兩比較採用單因素方差分析(ANOVA),LSD-t檢驗.結果 榦預前,與NGT組比較,IGT對照組及Ex組2hBG增高,骨骼肌GLUT4mRNA錶達降低,差異有統計學意義(均P ＜0.05).IGT對照組及Ex組骨骼肌細胞內棕黃色著色較少,NGT組著色廣汎,著色顆粒較多.艾塞那肽榦預4週後,與同期IGT對照組及榦預前Ex組比較,Ex組2 hBG下降,骨骼肌GLUT4 mRNA錶達增高,差異均有統計學意義(P＜0.05).榦預後Ex組較同期IGT對照組及榦預前Ex組骨骼肌細胞內棕黃色著色增多,主要錶達在細胞漿中.結論 艾塞那肽可上調骨骼肌GLUT4的錶達,促進骨骼肌葡萄糖的攝取,降低餐後血糖.
목적 탐토이고혈당소양태-1(glucagon-like peptide-1,GLP-1)수체격동제애새나태대당내량감저(impaired glucose tolerance,IGT)대서골격기포도당전운단백4(glucose transporter4,GLUT4)표체적작용.방법 54지Wistar대서,안조완전수궤화분조방법분위정상당내량조(NGT조,18지)화IGT조(36지),NGT조여상규사료,IGT조여고당고지사료.12주시IGT조모성공,성모후종IGT조수궤추취18지대서설위애새나태간예조(Ex조),매일2차피하주사애새나태(5μg/kg);령외18지설위IGT대조조.NGT조급IGT대조조균급여등체적생리염수피하주사.우간예전화간예후4주분별측공복혈당(fasting blood glucose,FBG)화찬후2h혈당(2 h BG);실시형광정량PCR검측골격기GLUT4mRNA적표체,면역조직화학법검측골격기GLUT4단백적표체.각조간량량비교채용단인소방차분석(ANOVA),LSD-t검험.결과 간예전,여NGT조비교,IGT대조조급Ex조2hBG증고,골격기GLUT4mRNA표체강저,차이유통계학의의(균P ＜0.05).IGT대조조급Ex조골격기세포내종황색착색교소,NGT조착색엄범,착색과립교다.애새나태간예4주후,여동기IGT대조조급간예전Ex조비교,Ex조2 hBG하강,골격기GLUT4 mRNA표체증고,차이균유통계학의의(P＜0.05).간예후Ex조교동기IGT대조조급간예전Ex조골격기세포내종황색착색증다,주요표체재세포장중.결론 애새나태가상조골격기GLUT4적표체,촉진골격기포도당적섭취,강저찬후혈당.
Objective To investigate the effect of glucagon-like peptide-1 agonist (exendin-4) on expression of glucose transporter 4 (GLUT4) in the skeletal muscle of rats with impaired glucose tolerance (IGT).Methodis 54 Wistar rats were randomly assigned into normal glucose tolerance group (NGT group,n =18) and impaired glucose tolerance group(IGT group,n =36).The rats in NGT group were fed with routine diet and the rats in IGT group were fed with high-sugar high-fat diet.At the 12th week,IGT models were tested successful.Then,half of the rats were allocated to intervention group (Ex group) and the rest were set as IGT control group.The rats in Ex group were subject to exendin-4 subcutaneous injection (5 μg/kg,twice daily).Each rat in NGT group and IGT control group was given the same volume of saline as injection.FBG and 2 h BG were measured before intervention and after 4 weeks.The expression of GLUT4 mRNA and GLUT4 in the skeletal muscle were respectively measured by real time quantitative polymerase chain reaction and immunohistochemistry.Inter-group comparison was conducted using analysis of variance (ANOVA) and least square deviation-test (LSD-t).Results Before intervention,compared with NGT-group,the 2 h BG in IGT control group and Ex group were higher,the GLUT4 mRNA of skeletal muscle in IGT control group and Ex group were lower (respectively P ＜ 0.05).The skeletal muscle cells in IGT control group and Ex group were less colored while the skeletal muscle cells in NGT group were colored extensively,and more colored granules.After 4 weeks of exendin-4 intervention,compared with IGT control group and Ex group of non-intervention,the 2 h BG level in Ex group was lower and the expression level of GLUT4 mRNA of skeletal muscle was higher (respectively P ＜ 0.05).After intervened with exendin-4 for 4 weeks,the GLU protein mainly expressed in cytoplasma of skeletal muscle cells.Its expression was higher in Ex group than in IGT group and in Ex group before intervention.Conclusion Exendin-4 may up-regulate the expression of GLUT4,increase glucose intake of the skeletal muscle,and reduce postprandial blood sugar.
