CAJ | 학술논문

目的观察血管内皮生长因子(vascular endothelial growth factor,VEGF)在分化型甲状腺癌(differentiated thyroid carcinoma,DTC)患者手术前后及复发时血清中的表达及其与血清甲状腺球蛋白(thyroglobulin,Tg)浓度之间的关系.方法采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)检测30例DTC(术后肺转移7例、局部复发23例)在术前、术后及复发时血清VEGF(serum VEGF,sVEGF)的水平及化学发光法检测血清Tg及TgA的浓度,并与30例正常对照组比较.结果肺转移组及局部复发组在手术前sVEGF比较差异无统计学意义(t=0.373,P＞0.05),与对照组比较差异有统计学意义(t值分别为8.597、14.140,P均＜0.05)；肺转移组及局部复发组在术后sVEGF明显降低,与术前比较差异有统计学意义(t值分别为5.977、11.500,P均＜0.05)；术后2组sVEGF与对照组比较差异均无统计学意义(t值分别为1.593、1.525,P均＞0.05)；肺转移组及局部复发组在复发前后sVEGF比较差异有统计学意义(t值分别7.387、12.160,P均＜0.05),复发后肺转移组sVEGF较局部复发组明显增高,差异亦有统计学意义(t=4.167,P＜0.05).2组血清Tg浓度与sVEGF表达成直线正相关(r=0.786,P＜0.01).结论 sVEGF在DTC术前及术后复发患者血清中高表达,可作为评估DTC生物学行为的一项重要指标.
목적 관찰혈관내피생장인자(vascular endothelial growth factor,VEGF)재분화형갑상선암(differentiated thyroid carcinoma,DTC)환자수술전후급복발시혈청중적표체급기여혈청갑상선구단백(thyroglobulin,Tg)농도지간적관계.방법 채용매련면역흡부법(enzyme-linked immunosorbent assay,ELISA)검측30례DTC(술후폐전이7례、국부복발23례)재술전、술후급복발시혈청VEGF(serum VEGF,sVEGF)적수평급화학발광법검측혈청Tg급TgA적농도,병여30례정상대조조비교.결과 폐전이조급국부복발조재수술전sVEGF비교차이무통계학의의(t=0.373,P＞0.05),여대조조비교차이유통계학의의(t치분별위8.597、14.140,P균＜0.05)；폐전이조급국부복발조재술후sVEGF명현강저,여술전비교차이유통계학의의(t치분별위5.977、11.500,P균＜0.05)；술후2조sVEGF여대조조비교차이균무통계학의의(t치분별위1.593、1.525,P균＞0.05)；폐전이조급국부복발조재복발전후sVEGF비교차이유통계학의의(t치분별7.387、12.160,P균＜0.05),복발후폐전이조sVEGF교국부복발조명현증고,차이역유통계학의의(t=4.167,P＜0.05).2조혈청Tg농도여sVEGF표체성직선정상관(r=0.786,P＜0.01).결론 sVEGF재DTC술전급술후복발환자혈청중고표체,가작위평고DTC생물학행위적일항중요지표.
Objective To evaluate the expression of serum vascular endothelial growth factor(sVEGF) in differentiated thyroid carcinoma(DTC)patients before and after thyroidectomy and when recurrence occured.To analyze the relationship between serum VEGF and serum thyroglobulin(Tg)level.Methods sVEGF levels of 30 cases of DTC (7 patients with pulmonary metastasis,23 patients with locoregional recurrence)were determined by enzyme-linked immunosorbent assay(ELISA) before and after thyroidectomy and when recurrence occured.The levels of serum Tg and thyroglobulin antibodies (TgA) were detected by chemiluminescence method.The results were compared with those of the 30 cases of normal controls.Results sVEGF levels had no significant difference between pulmonary metastasis group and locoregional recurrence group before operation (t =0.373,P ＞ 0.05),but they were significantly higher than those in the normal control group(t =8.597 and 14.14 respectively,all P ＜ 0.05).sVEGF levels in patients with pulmonary metastasis or locoregional recurrence were significantly reduced at the 3rd week after operation and the difference had statistical significance with those before operation(t =5.977,11.50,P ＜ 0.05).The difference of postoperative level of sVEGF in patients with pulmonary metastasis,locoregional recurrence and in patients of normal control group had no statistical significance (t =1.593,1.525,P ＞ 0.05).sVEGF levels in patients with pulmonary metastasis or locoregional recurrence were significantly higher after recurrence than those before recurrence (t =7.387,12.16,P ＜ 0.05).After recurrence sVEGF levels in patients with pulmonary metastasis were significantly higher than those in patients with locoregional recurrence (t =4.167,P ＜ 0.05) It showed a positive linear correlation between serum Tg level and sVEGF level in DTC patients with recurrence(r =0.786,P ＜ 0.01).Conclusion sVEGF is significantly elevated in DTC patients before operation and with postoperative recurrence,making it an important indicator to reveal the biological behaviors of DTC.