中华内分泌外科杂志
中華內分泌外科雜誌
중화내분비외과잡지
CHINESE JOURNAL OF ENDOCRINE SURGERY
2013年
6期
451-453
,共3页
梁辉%管蔚%马向华%田婷%张媛媛%吴鸿浩
樑輝%管蔚%馬嚮華%田婷%張媛媛%吳鴻浩
량휘%관위%마향화%전정%장원원%오홍호
十二指肠空肠旁路术%2型糖尿病%GK大鼠%胰高血糖素样肽-1%胰高血糖素原
十二指腸空腸徬路術%2型糖尿病%GK大鼠%胰高血糖素樣肽-1%胰高血糖素原
십이지장공장방로술%2형당뇨병%GK대서%이고혈당소양태-1%이고혈당소원
Duodenal-jejunal bypass%Type 2 diabetes mellitus%Goto-Kakizaki rats%Glucagonlike peptide-1%Proglucagon
目的 观察十二指肠空肠旁路术(duodenal-jejunal bypass,DJB)对2型糖尿病GK大鼠的降糖作用及对肠道胰高血糖素原(proglucagon,PG)表达的影响.方法 雄性GK和Wistar大鼠各18只,GK大鼠随机分为手术组(A组)假手术组(B组),Wistar大鼠随机分为手术组(C组)和假手术组(D组),每组9只.检测术前及术后各组空腹血糖、血清GLP-1水平,同时口服葡萄糖耐量试验,并计算血糖曲线下面积;术后8周取各组大鼠肠道组织标本,采用RT-PCR检测术后肠道PG mRNA表达水平.结果 术后第8周,A组空腹血糖由术前的(8.73±1.30) mmol/L下降到(5.86±0.57) mmol/L,差异有统计学意义(P＜0.05);血糖曲线下面积由术前(60.23±5.14)mmol· h/L下降到(47.80±1.79) mmol· h/L,差异有统计学意义(P＜0.05);空腹GLP-1由术前(7.69±0.74) pmol/L上升到(29.00±4.85) pmol/L,餐后GLP-1由术前(15.74 ±5.71) pmol/L上升到(45.78±7.26) pmol/L,差异有统计学意义(P均＜0.05);A组术后回肠PG mRNA水平明显高于B组,差异有统计学意义(P＜0.05).结论 DJB能显著改善2型糖尿病大鼠的糖代谢,而对正常大鼠糖代谢无影响,手术的降糖作用可能与肠道胰高血糖素原表达增加有关.
目的 觀察十二指腸空腸徬路術(duodenal-jejunal bypass,DJB)對2型糖尿病GK大鼠的降糖作用及對腸道胰高血糖素原(proglucagon,PG)錶達的影響.方法 雄性GK和Wistar大鼠各18隻,GK大鼠隨機分為手術組(A組)假手術組(B組),Wistar大鼠隨機分為手術組(C組)和假手術組(D組),每組9隻.檢測術前及術後各組空腹血糖、血清GLP-1水平,同時口服葡萄糖耐量試驗,併計算血糖麯線下麵積;術後8週取各組大鼠腸道組織標本,採用RT-PCR檢測術後腸道PG mRNA錶達水平.結果 術後第8週,A組空腹血糖由術前的(8.73±1.30) mmol/L下降到(5.86±0.57) mmol/L,差異有統計學意義(P＜0.05);血糖麯線下麵積由術前(60.23±5.14)mmol· h/L下降到(47.80±1.79) mmol· h/L,差異有統計學意義(P＜0.05);空腹GLP-1由術前(7.69±0.74) pmol/L上升到(29.00±4.85) pmol/L,餐後GLP-1由術前(15.74 ±5.71) pmol/L上升到(45.78±7.26) pmol/L,差異有統計學意義(P均＜0.05);A組術後迴腸PG mRNA水平明顯高于B組,差異有統計學意義(P＜0.05).結論 DJB能顯著改善2型糖尿病大鼠的糖代謝,而對正常大鼠糖代謝無影響,手術的降糖作用可能與腸道胰高血糖素原錶達增加有關.
목적 관찰십이지장공장방로술(duodenal-jejunal bypass,DJB)대2형당뇨병GK대서적강당작용급대장도이고혈당소원(proglucagon,PG)표체적영향.방법 웅성GK화Wistar대서각18지,GK대서수궤분위수술조(A조)가수술조(B조),Wistar대서수궤분위수술조(C조)화가수술조(D조),매조9지.검측술전급술후각조공복혈당、혈청GLP-1수평,동시구복포도당내량시험,병계산혈당곡선하면적;술후8주취각조대서장도조직표본,채용RT-PCR검측술후장도PG mRNA표체수평.결과 술후제8주,A조공복혈당유술전적(8.73±1.30) mmol/L하강도(5.86±0.57) mmol/L,차이유통계학의의(P＜0.05);혈당곡선하면적유술전(60.23±5.14)mmol· h/L하강도(47.80±1.79) mmol· h/L,차이유통계학의의(P＜0.05);공복GLP-1유술전(7.69±0.74) pmol/L상승도(29.00±4.85) pmol/L,찬후GLP-1유술전(15.74 ±5.71) pmol/L상승도(45.78±7.26) pmol/L,차이유통계학의의(P균＜0.05);A조술후회장PG mRNA수평명현고우B조,차이유통계학의의(P＜0.05).결론 DJB능현저개선2형당뇨병대서적당대사,이대정상대서당대사무영향,수술적강당작용가능여장도이고혈당소원표체증가유관.
Objective To investigate the effect of duodenal-jejunal bypass(DJB) on glucose metabolism and the expression of ileum proglucagon mRNA in Goto-Kakizaki (GK) type 2 diabetic rats.Methods 18 male GK rats and 1 8 male Wistar rats were randomly divided into 4 groups:GK operation group(group A),GK sham operation group(group B),Wistar operation group (group C),Wistar sham operation group (group D).There were 9 rats in each group.The fasting blood glucose and GLP-1 levels were measured before and at the 8th weekafter surgery.Oral glucose tolerance test was measured,and the area under blood glucose concentration curve wascalculated.Ileum tissues were obtained 8 weeks postoperatively and reverse transcriptase polymerase chain reac-tion was used to detect ileum PG mRNA expression after the operation.Results At the 8th week after surgery,the fasting blood glucose of group A decreased from(8.73 ± 1.30) mmol/L to(5.86 ±0.57) mmol/L(P ＜0.05).Area under blood glucose concentration curve decreased from preoperative(60.23 ± 5.14)mmol · h/L to (47.80 ±1.79) mmol· h/L(P ＜ 0.05).Fasting serum GLP-1 in group A increased from (7.69 ± 0.74) pmol/L to (29.00 ±4.85) pmol/L while postprandial GLP-1 increased from(15.74 ± 5.71) pmol/L to (45.78 ± 7.26) pmol/L (P ＜0.05).At the 8th week after surgery,the ileum PG mRNA level in group A was significantly higher than that in group B(P ＜ 0.05).Conclusions DJB can directly improve glucose metabolism in non-obese type 2 diabetic rats,and the operation has no hypoglycemic effect on normal blood glucose.The increased expression of ileum PG mRNA might contribute to the hypoglycemic effect of the surgery.
