CAJ | 학술논문

目的研究脆性组氨酸三联体基因(fragile histidine triad gene,FHIT)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)及其配对癌旁组织(non-carcerous epithelium,NCE)中的表达及其临床意义.方法采用逆转录聚合酶链反应(RT-PCR)检测60例PTC及相对应NCE中FHIT基因的表达情况.结果 PTC中FHIT基因表达阳性率为45.0％ (27/60),明显低于NCE 100.0％ (60/60),其差异有显著性(P＜0.01);PTC中FHIT基因mRNA表达水平与患者的病理学分级、TNM分期、淋巴结转移有相关性,差异有统计学意义(P＜0.05).结论 FHIT基因转录表达异常可能为一重要的分子改变参与了PTC的发展及演变,可望成为PTC病情监测及预后判断的一个生物学指标.
목적 연구취성조안산삼련체기인(fragile histidine triad gene,FHIT)재갑상선유두상암(papillary thyroid carcinoma,PTC)급기배대암방조직(non-carcerous epithelium,NCE)중적표체급기림상의의.방법 채용역전록취합매련반응(RT-PCR)검측60례PTC급상대응NCE중FHIT기인적표체정황.결과 PTC중FHIT기인표체양성솔위45.0％ (27/60),명현저우NCE 100.0％ (60/60),기차이유현저성(P＜0.01);PTC중FHIT기인mRNA표체수평여환자적병이학분급、TNM분기、림파결전이유상관성,차이유통계학의의(P＜0.05).결론 FHIT기인전록표체이상가능위일중요적분자개변삼여료PTC적발전급연변,가망성위PTC병정감측급예후판단적일개생물학지표.
Objective To study fragile histidine triad gene(FHIT) gene mRNA expression in 60 samples of papillary thyroid carcinoma(PTC) and matched adjacent non-concerous tissues (NCE),and to explore the role of FHIT gene in the development in PTC.Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect FHIT gene mRNA expression in 60 samples of PTC and matched NCE.Results In PTC tissues,the positive rate of FHIT gene mRNA expression was 45.0％ (27/60),lower than that in NCE tissues (100.0％,60/60).FHIT gene mRNA expression was correlated with pathology stage,TNM stage and lymph node metastasis (P ＜ 0.05).Conclusion FHIT loss and transcript abnormality might play important roles in proliferation and metastasis of PTC,and might be a useful marker for evaluating the biological behavior of PTC.