CAJ | 학술논문

目的研究FoxM1基因表达水平对甲状腺乳头状癌(papillary thyroid carcinoma,PTC)细胞活性和侵袭性有无影响,探讨FoxM1与PTC疾病的相关性.方法分别用hFoxM1-RNA干扰和硫链丝菌素处理PTC TPC-1细胞,观察处理后的癌细胞与未经处理的癌细胞在细胞活性、侵袭能力、迁移能力方面的变化,并使用Real-time PCR检测各组细胞中FoxM1基因的表达水平,观察硫链丝菌素对FoxM1基因表达水平的影响.结果在TPC-1细胞中,使用hFoxM 1-RNA干扰和硫链丝菌素处理均能使细胞活性明显下降(P＜0.05),用2种方法处理后,细胞侵袭能力分别降低了约29.2％及38.63％,细胞迁移能力分别降低了约46.0％及47.5％,且PCR结果表明,hFoxM1-RNA干扰和硫链丝菌素处理均明显抑制TPC-1细胞中FoxM1基因mRNA的表达,分别抑制55％及38％.结论 FoxM1能促进PTC细胞的侵袭转移,其作为潜在的肿瘤标志物值得关注,而硫链丝菌素能抑制肿瘤细胞中FoxM1的表达.
목적 연구FoxM1기인표체수평대갑상선유두상암(papillary thyroid carcinoma,PTC)세포활성화침습성유무영향,탐토FoxM1여PTC질병적상관성.방법 분별용hFoxM1-RNA간우화류련사균소처리PTC TPC-1세포,관찰처리후적암세포여미경처리적암세포재세포활성、침습능력、천이능력방면적변화,병사용Real-time PCR검측각조세포중FoxM1기인적표체수평,관찰류련사균소대FoxM1기인표체수평적영향.결과 재TPC-1세포중,사용hFoxM 1-RNA간우화류련사균소처리균능사세포활성명현하강(P＜0.05),용2충방법처리후,세포침습능력분별강저료약29.2％급38.63％,세포천이능력분별강저료약46.0％급47.5％,차PCR결과표명,hFoxM1-RNA간우화류련사균소처리균명현억제TPC-1세포중FoxM1기인mRNA적표체,분별억제55％급38％.결론 FoxM1능촉진PTC세포적침습전이,기작위잠재적종류표지물치득관주,이류련사균소능억제종류세포중FoxM1적표체.
Objective To study correlation of FoxM1 expression level with the activity and invasiveness in papillary thyroid carcinoma (PTC)cell line TPC-1.Methods TPC-1 cells were treated by hFoxM1-RNA interference or thiostrepton.The changes of cell activity,invasiveness and migration of treated TPC-1 cells were observed.Real-time PCR was used to measured the expression of FoxM1 in TPC-1 cells or thiostrepton treated TPC-1 cells.Results In TPC-1 cells,either hFoxM1-RNA interference or thiostrepton significantly inhibited the cell activity.The cell invasiveness decreased 29.2％ or 38.63％ and migration decreased 46.0％ or 47.5％ after the treatment of hFoxM1-RNA interference or thiostrepton.Treatment of hFoxM1-RNA interference or thiostrepton significantly inhibited mRNA level of FoxM1 in TPC-1 cells,the inbition was 55％ or 38％.Conclusions FoxM1 can promote the invasion and metastasis of PTC cells.It suggests that FoxM1 can be used as a potential tumor marker,and thiostrepton can inhibit FoxM1 expression in tumor cells.