CAJ | 학술논문

目的探讨正常人及初诊2型糖尿病(type 2 diabetes mellitus,T2DM)患者脂肪、肌肉组织中分泌型卷曲相关蛋白5(secreted frizzled-related protein 5,SFRP5)的差异性表达及其与胰岛素抵抗(insulin resistance,IR)的关系.方法按照1999年WHO的关于糖尿病的诊断标准选取初诊T2DM患者12例,及年龄、性别相匹配的正常人12例.所有研究对象均来自外科腹部择期手术患者,在同样手术条件下获取腹部大网膜脂肪组织和肌肉组织.常规测量体重、身高、腰围、臀围、血压、胰岛素(Fins)、血糖、糖化血红蛋白(HbA1C)、血脂等,计算胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(HOMA-β),用QRT-PCR方法和Western印迹方法检测SFRP5在不同人群中的差异性表达及其与IR的关系.结果 ①T2DM组与正常对照组相比,T2DM组收缩压(SBP),甘油三酯(TG),空腹血糖(FPG),糖负荷后2h血糖(2h PG),Fins,稳态模型评价HOMA-IR,HbA1C均升高.②T2DM组脂肪组织中SFRP5 mRNA和蛋白表达高于正常对照组,差异有统计学意义(2.7倍,5.5倍,均P＜0.01),但在肌肉组织中差异无统计学意义.③脂肪组织SFRP5 mRNA和蛋白质高于肌肉组织.结论脂肪组织SFRP5 mRNA和蛋白质表达高于肌肉组织提示脂肪组织可能是SFRP5的主要来源.T2DM组脂肪组织中SFRP5 mRNA和蛋白表达高于正常对照组提示SFRP5可能与IR严重程度相关.
목적 탐토정상인급초진2형당뇨병(type 2 diabetes mellitus,T2DM)환자지방、기육조직중분비형권곡상관단백5(secreted frizzled-related protein 5,SFRP5)적차이성표체급기여이도소저항(insulin resistance,IR)적관계.방법 안조1999년WHO적관우당뇨병적진단표준선취초진T2DM환자12례,급년령、성별상필배적정상인12례.소유연구대상균래자외과복부택기수술환자,재동양수술조건하획취복부대망막지방조직화기육조직.상규측량체중、신고、요위、둔위、혈압、이도소(Fins)、혈당、당화혈홍단백(HbA1C)、혈지등,계산이도소저항지수(HOMA-IR)、이도소민감지수(HOMA-β),용QRT-PCR방법화Western인적방법검측SFRP5재불동인군중적차이성표체급기여IR적관계.결과 ①T2DM조여정상대조조상비,T2DM조수축압(SBP),감유삼지(TG),공복혈당(FPG),당부하후2h혈당(2h PG),Fins,은태모형평개HOMA-IR,HbA1C균승고.②T2DM조지방조직중SFRP5 mRNA화단백표체고우정상대조조,차이유통계학의의(2.7배,5.5배,균P＜0.01),단재기육조직중차이무통계학의의.③지방조직SFRP5 mRNA화단백질고우기육조직.결론 지방조직SFRP5 mRNA화단백질표체고우기육조직제시지방조직가능시SFRP5적주요래원.T2DM조지방조직중SFRP5 mRNA화단백표체고우정상대조조제시SFRP5가능여IR엄중정도상관.
Objective To investigate the change expression levels of secreted frizzled related protein 5 (SFRP5) mRNA and protein in human adipose,and muscle tissues and the relationship between SFRP5 and insulin resistance in different glucose tolerance subjects.Methods Twelve type 2 diabetes mellitus(T2DM) patients and 12 aged and sex-matched healthy control subjects were enrolled.T2DM patients were diagnosed by1999 WHO criterion.Under the same conditions,the omental adipose and muscle tissues from these patients undergoing elective abdominal surgery were obtained.Expressions of sfrp5 protein and mRNA in adipose and muscle tissues were determined by semi—quantitive RT-PCR and Western blot while body height,weight,waist circumference,hipcircumference,blood pressure,lipodemia,fasting plasma glucose,fasting insulin,glycosylated hemoglobin,fasting serum insulin(FINS),HOMA-β,HOMA-insulin resistance index(HOMA-IR) etc were investigated.Results 1.SBP,TG,FPG,2 h PG,Fins,HOMA-IR,HbA1C were significantly higher in T2DM groups than in the control group.2.The expressions of SFRP5 mRNA and protein in omental adipose tissues were significantly higher inT2DM group than in the control group(P ＜ 0.01).3.The level of SFRP5 mRNA and protein expressions were higher in omental adipose tissue than in the muscle tissues.Conclusions The fact that mRNA and protein expression level of SFRP5 was higher in adipose tissue than in muscle indicates adipose tissue may be the main source of SFRP5.The expressions of SFRP5 mRNA and protein were higher inT2DM group than in the control group indicates sfrp5 may play a role in insulin resistance.