CAJ | 학술논문

目的探讨白细胞介素1d(IL-1α)基因单核苷酸多态性(SNP)与甲型H1N1流感易感性的关系.方法从IL-1α基因启动子区域中选取4个SNP位点rs1800587、rs2856836、rs2856838、rs3783525,针对以上位点建立基于飞行时间质谱分析技术(TOF-MS)鉴定SNP的方法,对167例H1N1流感患者(H1N1组)和192例健康对照人群(对照组)进行检测.确定各SNP位点的等位基因和基因分型;对比两组等位基因和各基因型的频率.结果 rs1800587、rs2856836、rs2856838位点具有T和C两种等位基因,包括TT、TC、CC 3种基因型.rs3783525具有A和T两种等位基因,包括AA、AT和TT3种基因型.H1N1组和对照组rs 1800587位点的等位基因频率差异有统计学意义(x2=12.69,P=0.000,OR=2.424,95％CI=1.472 ～ 3.993),rs2856836,rs2856838,rs3783525等位基因频率在H1N1组和对照组中差异则无统计学意义.H1N1组rs1800587位点CC纯合子频率低于对照组[72.5％(121/167)比87.0％(167/192),P＜0.05],杂合子(TC)频率高于对照组[25.1％(42/167)比12.5％(24/192),P＜0.05],而两组TT纯合子频率差异则不具有统计学意义.H1N1组rs2856836位点杂合子(TC)频率高于对照组[25.7％(43/167)比17.2％(33/192),P＜0.05],TT基因型与CC基因型频率在两组间差异均没有统计学意义.rs2856838位点对照组TT纯合子频率低于H1N1组[4.2％(8/192)比10.8％(18/167),P＜0.05],其余2种基因型TC与CC在两组间差异没有统计学意义.H1N1组rs3783525位点TTT纯合子频率高于对照组[21.0％ (35/167)比13.0％(25/192),P＜0.05],两组间的另两种基因型TC与CC差异也没有统计学意义.结论 IL-1α基因多态性位点rs1800587、rs2856836、rs2856838、rs3783525与甲型H1N1流感易感性相关.
목적 탐토백세포개소1d(IL-1α)기인단핵감산다태성(SNP)여갑형H1N1류감역감성적관계.방법 종IL-1α기인계동자구역중선취4개SNP위점rs1800587、rs2856836、rs2856838、rs3783525,침대이상위점건립기우비행시간질보분석기술(TOF-MS)감정SNP적방법,대167례H1N1류감환자(H1N1조)화192례건강대조인군(대조조)진행검측.학정각SNP위점적등위기인화기인분형;대비량조등위기인화각기인형적빈솔.결과 rs1800587、rs2856836、rs2856838위점구유T화C량충등위기인,포괄TT、TC、CC 3충기인형.rs3783525구유A화T량충등위기인,포괄AA、AT화TT3충기인형.H1N1조화대조조rs 1800587위점적등위기인빈솔차이유통계학의의(x2=12.69,P=0.000,OR=2.424,95％CI=1.472 ～ 3.993),rs2856836,rs2856838,rs3783525등위기인빈솔재H1N1조화대조조중차이칙무통계학의의.H1N1조rs1800587위점CC순합자빈솔저우대조조[72.5％(121/167)비87.0％(167/192),P＜0.05],잡합자(TC)빈솔고우대조조[25.1％(42/167)비12.5％(24/192),P＜0.05],이량조TT순합자빈솔차이칙불구유통계학의의.H1N1조rs2856836위점잡합자(TC)빈솔고우대조조[25.7％(43/167)비17.2％(33/192),P＜0.05],TT기인형여CC기인형빈솔재량조간차이균몰유통계학의의.rs2856838위점대조조TT순합자빈솔저우H1N1조[4.2％(8/192)비10.8％(18/167),P＜0.05],기여2충기인형TC여CC재량조간차이몰유통계학의의.H1N1조rs3783525위점TTT순합자빈솔고우대조조[21.0％ (35/167)비13.0％(25/192),P＜0.05],량조간적령량충기인형TC여CC차이야몰유통계학의의.결론 IL-1α기인다태성위점rs1800587、rs2856836、rs2856838、rs3783525여갑형H1N1류감역감성상관.
Objective To determine the association between interleukin-1 alpha (IL-1α) gene single nucleotide polymorphism(SNP) and susceptibility to type A H1N1 influenza.Methods Four SNPs (rs1800587,rs2856836,rs2856838 and rs3783525) were selected from IL-1α promoter region for subsequent establishment of the methodology for identifying SNP based on time of flight-mass spectroscopy (TOF-MS),which was employed to genotype patients with H1N1 (n=167) and healthy controls(n=192).The alleles of respective SNP were genotyped for comparison on their frequency.Results The rs1800587,rs2856836 and rs2856838 SNPs consisted of T and C alleles and the genotypes of TT,TC and CC.By contrast,the rs3783525 included A and T alleles that comprised of AA,AT and TT genotypes.The difference in allele frequency between H1 N 1 groups and healthy controls reached statistical significance(x2=12.69,P=0.000,OR=2.424,95％CI∶ 1.472-3.993),whilst the between-group difference in allele frequency was not statistically significant for rs2856836,rs2856838 and rs3783525.Patients with H1N1 yielded a lower allele frequency of CC homozygote for rs 1800587 [72.5％ (121 / 167) vs 87.0％ (167/192),P＜0.05] yet higher frequency of TC heterozygote [25.1％ (42/167) vs 12.5％ (24/192),P＜0.05].There was no significant difference in the frequency of TT homozygote.A higher frequency of TC heterozygote for rs2856836 [25.7％(43/167) vs 17.2％ (33/192),P＜0.05] was noted in patients with H1N1,in whom the frequency of TT and CC genotypes did not differ statistically.Patients with H1N1 were linked to a higher frequency of TT homozygote for rs2856838 [4.2％(8/192) vs 10.8％(18/167),P＜0.05] yet did not differ markedly in the genotypes of TC and CC.Similarly,a higher frequency of TT homozygote for rs3783525 was found in patients with H1N1 [21.0％(35/167) vs 13.0％(25/192),P＜0.05],who did not evidence a considerable difference in genotypes TC and CC for two miscellaneous alleles.Conclusion The IL-1α gene SNPs,namely rs1800587,rs2856836,rs2856838 and rs3783525 are associated with the susceptibility to type A H1N1 influenza.